DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and rapgef2b

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	XP_017214661.1	Gene:	rapgef2b / 557693	ZFINID:	ZDB-GENE-030131-6959	Length:	1634	Species:	Danio rerio

Alignment Length:	1269	Identity:	292/1269 - (23%)
Similarity:	476/1269 - (37%)	Gaps:	383/1269 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly    13 PCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGRFWYAVLGGS 77
            |.||..:|:|::...|..:|.|..|....|:.:.....||..|....|:...:.|..||.:|.||
Zfish    17 PSDRTQQDLEIVYSYLHGMEALSNLREHQLRIMCETVRYERHEANEVLYYPDDIGTCWYILLSGS 81

  Fly    78 LEVRYHAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRVEQQDFRLIWEKN 142
            :.:          |.::.|..    ::||:.....|.|....:.....|::.|:..|      :|
Zfish    82 VFI----------KESMFLPR----SSFGKRSAGSLRRGCECIVLEPSEMIVVDYMD------EN 126

  Fly   143 KELMNDIFTNCKLKNGFGPSVQATAAATSPTKRPLSPDHPNPALPITETPSPAMN---RMGW--- 201
            :|....               ||:...:....|.::.......:..|..|.||..   ..|:
Zfish   127 EEYFQR---------------QASHRQSRRRFRKINQKGERQTIIDTVDPYPAGKPPVARGYHTE 176

  Fly   202 ALRTLLVADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSP-IVHTRAQAAGMWQALLEEGVLA 265
            .::..|.||.|.                  ..|.|   .:.| :.|..:..:|           .
Zfish   177 CVKAQLPADFSR------------------LHLAD---GIHPQVTHVSSSHSG-----------C 209

  Fly   266 HVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQA-----EDLGAANEHIREALSALFQRGPDATLR 325
            .:..:........:|:....|.|....:|:|:.     ||  ...|.|..|...|..|.   .:|
Zfish   210 SITSDSGSSSLSDIYQATESEPGDMDLSGLPETAVDSEED--DDEEDIERASDPLMSRD---IVR 269

  Fly   326 MILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDEGRSW 390
            ..|.|...:||.:::|.:.|.:..:.|.::::.|::|||.::.||....:||||:.|.|:|..||
Zfish   270 DCLEKDPMDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSW 334

  Fly   391 YILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNRILRD 455
            .::|.|||:|. |.......|..|:.||....:........:..|.::|..:.:.::.:..||..
Zfish   335 SVILNGSVEVT-HPDSRNEILCMGNSFGVSPTMEKEYMKGVMKTKVDDCQFVCIAQQDYCCILNQ 398

  Fly   456 VEANTLRLQEHGKDVLV-----LERVAKQRGQHSAFKYTVMSGTPAKMLEHLLETRLGQSVGGMD 515
            ||.|..:::|.|:.|:|     |:|...::|      :.|:.||..::..||:|.   .||  :|
Zfish   399 VEKNMQKVEEEGEIVMVKEHRELDRTGTRKG------HIVIKGTSERLTMHLVEE---HSV--VD 452

  Fly   516 P-FLDDFLLTHIVFMPVVQLVDE-LANYFHCDAHEDAQTP-----------------------ED 555
            | :::|||||:..|:|...:|.: |..:||..:..|..|.                       |:
Zfish   453 PTYIEDFLLTYRTFLPSPMVVGKRLLEWFHDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTHFLEE 517

  Fly   556 REY------------IINF------KKRVIQFMQ------------------------------- 571
            .||            ::|.      |.||:...:
Zfish   518 FEYNLEREKMCGHLRLLNIACAAKAKLRVVTLTKPSREAPLAFTLLGGSEKGFRIFIDSVEPGSK 582

  Fly   572 ---------KWVMAVRHAAFEE------------------------------------------- 584
                     ..::.|....||.
Zfish   583 AAEAGLKRGDQILEVNGQNFENVPLSKANEILKNNTHLSITVKTNLLVFKELLARPDQDQEQEQE 647

  Fly   585 ------------PSVCDF-------IEDLAAEVEADPDL---------------NEETSIVHNVL 615
                        |.:.|.       |.|||.:||...||               |:...|....|
Zfish   648 EEPDRKNGAPHIPKIGDIKKASRYSIPDLAVDVEQVMDLEKASKKAKANSAGGRNKLKKIFDKTL 712

  Fly   616 TQ-----------MARYQEDRNQNAGQKWKLPPNGQPICLFSGNATPSKTVIRPD---------- 659
            |.           :.:.|:|......|..::|| ..|:   |||.:.|    .||
Zfish   713 TSILPPKPYNDVGVGQSQDDSIVGLKQSKQIPP-ALPV---SGNLSSS----NPDLLQSHHRILD 769

  Fly   660 ------------DDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVLVEVKSN 712
                        .|.:.||:.||.....:.....|||:.:.|.|..:..|....|...|.||...
Zfish   770 FNNQPEAVPINMSDQVLRVFKADQQSRYIMIGKDTTAKEVVAQAIREFALTAAAEAYSLCEVSVT 834

  Fly   713 GERSVFKDNDVSIPTGLS-------LNGRLFVSVKDHLDALTQLQE---QECPTEGVDIDLEILS 767
            .| .|.|..  .:|..||       |:||.:  :|.:::..|...:   ||...|. .|.|..||
Zfish   835 PE-GVIKQR--RLPEQLSKLADRIQLSGRYY--LKSNMETETLCSDEDAQELLRES-HISLLQLS 893

  Fly   768 TKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITA--NLDVFLRRFNEVQYWIVTELVSTP 830
            |.|:|..:::..::||.|:...|.:...|...  .::|.  :|.:|....|...:|:.||:|...
Zfish   894 TVEVATQLSMRAFELFCAIEPTEYIDDLFKLK--TRLTGPPSLKLFEEAINRETFWVATEVVREA 956

  Fly   831 SLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEALI 895
            :..||:.:|:.|||:|.:|:|.:|.|:.||::.||:...||||:.||||:|||:.|:|.:.:.|.
Zfish   957 NQLKRMKIVKHFIKIALHCRECKNFNSMFAIISGLNLAPVSRLRGTWEKLPSKYEKLFSDLQDLF 1021

  Fly   896 DPSRNHRAYRVFVG--KLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRTI-- 956
            |||||...||..:.  .||||:||..|::.||:||.||||.:.:||||||||:.|:|:.:|.:
Zfish  1022 DPSRNMAKYRNLLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGR 1086

  Fly   957 --------------RFCRSRSLGLEPPSPKSEG-------------------------------- 975
                          |..:.||||     ..|:|
Zfish  1087 MAAVNMDPALMFRTRKKKWRSLG-----SLSQGSANAAVLDVTQTGGHKKRVRRSSFLNAKKLYE 1146

  Fly   976 ------EVRSYISSFRVIDNQRVLTAMSQKVEPT 1003
                  :|:.|:|:..:..|:..|..:|.:.||:
Zfish  1147 DAQMARKVKQYLSNLTLETNEESLQTLSMQCEPS 1180

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	26/117 (22%)
		CAP_ED	36..146	CDD:237999	23/109 (21%)
		DEP_Epac	199..330	CDD:239884	24/139 (17%)
		CAP_ED	352..463	CDD:237999	33/110 (30%)
		Crp	373..525	CDD:223736	47/157 (30%)
		RasGEF_N	489..594	CDD:279012	36/249 (14%)
		UBQ	665..>714	CDD:294102	14/48 (29%)
		RasGEF	766..1002	CDD:214539	93/293 (32%)
rapgef2b	XP_017214661.1	None

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810