Protein Alignment Epac and rasgrf2b

DIOPT Version :10

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: XP_009300046.1
• Gene: rasgrf2b / 553520
• ZFINID: ZDB-GENE-060504-1
• Length: 1275
• Species: Danio rerio
• Alignment Length: 1135
• Identity: 210/1135 - (18%)
• Similarity: 368/1135 - (32%)
• Gaps: 404/1135 - (35%)

  Fly   149 IFTNCKLKNGFGPSVQATAAATSPTKRPLSPD-------------------HPNPALPITETPS- 193
            |..||.|:        ....|.|..|.|:|.|                   |......|...|:
Zfish   260 ILVNCFLR--------PLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTL 316

  Fly   194 ----------PAMN-------RMGWALRTLLVADNSSCLKDRKVSGKLIRK------CA------ 229
                      |.:|       ...::|:.|     ::|.::|... ||:::      |.
Zfish   317 VLADLFDILLPMLNIYQEFVRNHQYSLQVL-----ANCKQNRDFD-KLLKQYESNAACEGRMLET 375

  Fly   230 ----PGTELVDWLVNL------SPIVHTRAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRI 284
                |..::..:::.|      :|..|...::....::.|||                 |.|...
Zfish   376 FLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEE-----------------LSRVMH 423

  Fly   285 DEEGGTAAAGVPQAEDLGAANEHIRE--ALSALFQRGPDATL---------RMILRKPSHERTSE 338
            ||...|               |:||:  |:..:...|.|..|         ..:::.||.||
Zfish   424 DEVSDT---------------ENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVER--- 470

  Fly   339 ELELVFEELVHIAALSHL---STSIKRE-LSSIFVFEAHAQAGTILFNQGDEGRSWYILLKGSVD 399
                        ..||.:   |.|:|:| ....|:|..|     .|......|...::|.:|.|.
Zfish   471 ------------GKLSKVRLGSLSLKKEGERQCFLFTKH-----FLICTRSSGGKLHLLKQGGVL 518

  Fly   400 VVIHGKGTVATL------------KTGDDFGKLAL-----INDAPRAATIVLKENNCHLLRVDK- 446
            .:|.     .||            .:|..||.|..     .:||| |.|:||...:    |.:|
Zfish   519 SLIE-----CTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAP-AFTVVLLAPS----RQEKA 573

  Fly   447 ---EHFNRILRDVEANTLRLQ--EHGKDVLVLERVAKQRGQHS-----AFKYTVMS--------G 493
               ...::.:.::..|.|...  |....|.|...:......|.     .|..|:.|        .
Zfish   574 AWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYA 638

  Fly   494 TPAKMLEHLLETRLGQSVGGMDPFLDDFLLTHIVFMPVVQLVDELANYFHCDAHEDAQTPEDREY 558
            :..::||.|.:.|. .|:    .||:.||.|:.:|.....::::||:.
Zfish   639 SVERLLERLTDLRF-LSI----DFLNTFLHTYRIFTTATVVMEKLADI----------------- 681

  Fly   559 IINFKKRVIQFMQKWVMAVRHAAFEEPSVCDFIEDLAAEVE---ADPDLNEETSIVHNVLTQMAR 620
               :||.....    .:.|::.:|:..|:.....|.:.::.   .|...:.|.....|...:...
Zfish   682 ---YKKPFTSI----PVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGD 739

  Fly   621 YQEDRNQNAGQKWKLPPNGQPICLFSGNATPSKT------------------------------- 654
            :..|::....:|:..||   |:.:.|..::|.:|
Zfish   740 HVNDKSPRLCRKFSSPP---PLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTS 801

  Fly   655 ---VIRP-------------------------------DDD------------------------ 661
               .|.|                               ||:
Zfish   802 ANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRAVLES 866

  Fly   662 ------------------------------IIFR---VYCADHTYCTL----RFPMHTTA----- 684
                                          :.:|   |..|:::.|::    .|.:.|.|
Zfish   867 VSLDKFIPESPQASEPGEISPCRSPSTPRHLRYRQSGVQTAENSRCSVSPASAFAIATAAAGHSS 931

  Fly   685 -------------ELIKACAADKLQLN-------RGPEDLVLVEVKSNGE---------RSVFKD 720
                         |.|...||....||       :..:|.     :.|||         ..|.:|
Zfish   932 PPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDF-----EMNGELKMGVICLLEEVLRD 991

  Fly   721 NDVSIPTGLSLNGRLF--VSVKDHLDALTQ----LQEQECPTEGVDIDLEILSTKELAYHITLFE 779
            .|: :|........:.  :|..|..||..:    ||..|||....   .|.||..|:|..|||.:
Zfish   992 PDL-LPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAEC---FESLSAMEIAEQITLLD 1052

  Fly   780 WDLFWAVHEYELLYHTFGRHHFGKITANLDVFLRRFNEVQYWIVTELVSTPSLSKRVGLVRKFIK 844
            ..:|.::...|.|...:.:....:.|..:....:.||::...:.::::|...:..|.|.:.|::.
Zfish  1053 HIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVA 1117

  Fly   845 LAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEALIDPSRNHRAYRVFVG 909
            :|..|:...|.|....:...|:..|:.||::||.|:..:.:.:..:.:..:......:..|..:.
Zfish  1118 VADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLK 1182

  Fly   910 KLQPPLIPFMPLLLKDMTFAHEG--NKTSLDGLVNFEKMHMMAQTMRTIRFCRSRSLGLEPPSPK 972
            ...||.:|::.:.|.|:.|..||  |.|. :|||||.||.|::..:|.||..:.....:| ..||
Zfish  1183 NCNPPCVPYLGMYLTDLAFIEEGTPNFTE-EGLVNFSKMRMISHIIREIRQFQQTPYRIE-HQPK 1245

  Fly   973 SEGEVRSYISSFRVIDNQRVLTAMSQKVEP 1002
                |..|:....:|.::..|..:|.|:||
Zfish  1246 ----VTQYLLDKTLIMDEDTLYDLSLKIEP 1271

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	CAP_ED	36..146	CDD:237999
		DEP_Epac	199..330	CDD:239884	26/163 (16%)
		CAP_ED	352..463	CDD:237999	32/135 (24%)
		RasGEF_N	489..594	CDD:459873	20/112 (18%)
		Ubl1_cv_Nsp3_N-like	652..>722	CDD:475130	24/229 (10%)
		RasGEF	766..1002	CDD:214539	59/237 (25%)
rasgrf2b	XP_009300046.1	PH_RasGRF1_2	19..154	CDD:270081
		RhoGEF	243..424	CDD:214619	31/194 (16%)
		PH	476..583	CDD:459697	28/121 (23%)
		RasGEF_N	634..>682	CDD:459873	13/72 (18%)
		REM	<938..1010	CDD:470601	14/77 (18%)
		RasGEF	1036..1272	CDD:214539	62/242 (26%)