DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and Rapgef5

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	NP_001382072.1	Gene:	Rapgef5 / 362799	RGDID:	1561668	Length:	880	Species:	Rattus norvegicus

Alignment Length:	881	Identity:	304/881 - (34%)
Similarity:	467/881 - (53%)	Gaps:	110/881 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly   164 QATAAATSPTKRPLSP--DHPNPALPITETPSPAMNRMGWALRTLLVADNSSCLKDR-KVSGKLI 225
            ::.|.|.:.::|..:|  || :|:....|..|.|    |.|||.:::...:..:||| .:.|...
  Rat    66 RSAAGARTLSRRISNPYLDH-SPSQIYGENSSCA----GRALRNIIILQAADLVKDRVNLKGFYR 125

  Fly   226 RKCAPGTELVDWLVNLSPIVHTRAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGT 290
            |.|. |:||||||:...|.|..|:.|.|:||.||:.|:::.|::...|:|....|:|..||
  Rat   126 RSCV-GSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQFSSDE---- 185

  Fly   291 AAAGVPQAEDLGAANEHIREALSALFQRGPDATLRMILRKP--------SHERTSEELELVFEEL 347
                   ...|....|...|     :|:|....|.::...|        ||:: :|:.|...:|:
  Rat   186 -------CSYLYCEFEREEE-----WQKGVKLLLELVHLIPARAGICDLSHQK-AEDSEESSDEI 237

  Fly   348 VHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDEGRSWYI-LLKGSVDVVIHGKGTVATL 411
                 |:.|:::::|||:::...:|...|    ..|.||....:: :.:.:.|......|.:..|
  Rat   238 -----LARLTSAVQRELAAVIALKARKSA----IEQDDENTDKHVTVTEANDDAPDPQAGVMCKL 293

  Fly   412 KTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNRI-LRDVEANTLRLQEHGKDVLVLER 475
            :..||.|::.|::.        |...||..|:.||:...:. .:|.|...:::::.|:.||||::
  Rat   294 QERDDIGRIELVHK--------LARENCQFLQTDKKEPEKSEQQDDEVTMVQVKDQGQSVLVLKK 350

  Fly   476 VAK------QRGQHSAFKYTVMSGTPAKMLEHLL-----------ETRLGQSVGGMDPFLDDFLL 523
            ||.      .....|..:|.|:||||.|:|||||           ||         :..||||||
  Rat   351 VASCGPAPTTGSAESDARYVVVSGTPEKILEHLLNDLHLAEVQHKET---------ETLLDDFLL 406

  Fly   524 THIVFMPVVQLVDELANYFHCDAHEDAQTPEDREYIINFKKRVIQFMQKWVMAVRHAAFEEPSVC 588
            |:.|||....|...|..::....:   |..|:...:...|::|:..:.:|:...:....|:....
  Rat   407 TYTVFMTTDDLCQALLRHYSAKKY---QGEEENSDVPCRKRKVLHLVSQWISLYKDWLHEDEHSK 468

  Fly   589 DFIEDLAAEVEAD----PDLNEETSIVHNVLTQMARYQED------RNQNAGQKWKLPPNGQPIC 643
            .|::.:...|..|    |.|.:|......:|....|:..|      :|:....::.|..|
  Rat   469 MFLKTIYRNVLDDVYEYPILEKELKEFQKILGMHRRHTVDEYSPQKKNKALFHQFSLKEN----- 528

  Fly   644 LFSGNATPSKTVIRPDDDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVLVE 708
            ......|.::|     ::|...||..:|:|.:::..:.:||:.|....|:|||  |..|||.||.
  Rat   529 WLQHRGTVAET-----EEIFCHVYVTEHSYISVKAKVSSTAQEILKVVAEKLQ--RAEEDLALVA 586

  Fly   709 VKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGVDIDLEIL--STKEL 771
            :..:||:..|:.||::|...|..:||::|..||..|.|....|.|   |.....:.||  :|.:|
  Rat   587 IMFSGEKHEFQPNDLAISKSLEASGRIYVYRKDLADTLNPFAENE---ESQQRSMRILGMNTWDL 648

  Fly   772 AYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITANLDVFLRRFNEVQYWIVTELVSTPSLSKRV 836
            |..:..|:|.||.::||.||:|.||.|...|:.|.||.:.|:|.||||.|:.||::....|.|||
  Rat   649 ALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTVNLSLLLQRCNEVQLWVATEILLCSQLGKRV 713

  Fly   837 GLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEALIDPSRNH 901
            .||:||||:||:||..||||:|||:||||:..:||||.|||||||.||:|:|.|.|:|.|||.||
  Rat   714 QLVKKFIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNH 778

  Fly   902 RAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRTIRFCRSRSLGL 966
            :|||....|::||.||||||||||:||.||||||.||.||||||:||:|.|:||:|.||:...|.
  Rat   779 KAYRDAFKKMKPPKIPFMPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGS 843

  Fly   967 EPPSPKSEGEVRSYISSFRVIDNQRVLTAMSQKVEP 1002
            : .|||.:.|::||::...|||:|:.|..:|.::||
  Rat   844 D-MSPKEQQELKSYVNHLYVIDSQQALFELSHRLEP 878

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736
		CAP_ED	36..146	CDD:237999
		DEP_Epac	199..330	CDD:239884	40/131 (31%)
		CAP_ED	352..463	CDD:237999	25/112 (22%)
		Crp	373..525	CDD:223736	47/170 (28%)
		RasGEF_N	489..594	CDD:279012	32/115 (28%)
		UBQ	665..>714	CDD:294102	17/48 (35%)
		RasGEF	766..1002	CDD:214539	131/237 (55%)
Rapgef5	NP_001382072.1	DEP_Epac	98..221	CDD:239884	42/143 (29%)
		RasGEFN	368..499	CDD:214571	38/142 (27%)
		RasGEF	640..879	CDD:214539	134/240 (56%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	68	1.000	Domainoid score	I9496
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR23113
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	8	7.920