Protein Alignment Epac and Rapgef6

DIOPT Version :10

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: XP_017452748.1
• Gene: Rapgef6 / 303141
• RGDID: 1307645
• Length: 1619
• Species: Rattus norvegicus
• Alignment Length: 1269
• Identity: 278/1269 - (21%)
• Similarity: 480/1269 - (37%)
• Gaps: 405/1269 - (31%)

  Fly     8 AMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGRFWYA 72
            |:.|:|.:|...|:.:|...|..:|.|..|....|:.::....||.......||.:....|.||.
  Rat    12 ALRKKPPERTPEDLNIIYSYLHGMEILSNLREHQLRLMSTRARYERYNGNQMLFCSETIARCWYI 76

  Fly    73 VLGGSLEVRYHAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRVEQQDFRL 137
            :|.||:.|          |.::.|.....|..||              .|..|:.|.:|..:. :
  Rat    77 LLSGSVLV----------KDSMVLPPCSFGKQFG--------------GKRGCDCLVLEPSEM-I 116

  Fly   138 IWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKRPLSPDHPN-----------------PA 185
            :.|.:|:..:.|             :|....|....:|....::..                 ||
  Rat   117 VVENSKDNEDSI-------------LQREIPARQSRRRFRKINYKGERQTIIDGVDVNNYLSLPA 168

  Fly   186 ----LPITETPSPAMNRMGWALRTLLVADNSSC--LKDRKVSGKLIRKCAPGTELVDWLVNLSPI 244
                :.:|:.|.|.:        |.:.:..|.|  ..|...|                  :||.|
  Rat   169 DLTKMHLTDNPHPQV--------THVTSSQSGCSIASDSGSS------------------SLSDI 207

  Fly   245 VH-TRAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGAANEHI 308
            .. |.::...:....|.||.:...::|              :||.........|..||      :
  Rat   208 YQATESEVGDVDLTRLPEGPVDSEDEE--------------EEEEEIDRTDPLQGRDL------V 252

  Fly   309 REALSALFQRGPDATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAH 373
            ||.|.               ::|: ::|.:::|.:.|.:..:.|.::::.|::|||.|:.:||..
  Rat   253 RECLE---------------KEPA-DKTDDDVEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVV 301

  Fly   374 AQAGTILFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENN 438
            .|||.::...|.|..|||::|.|:|: :.|..|.:..|..|:.||.:..::.......:..|.::
  Rat   302 EQAGAVILEDGQELDSWYVILNGTVE-ISHPDGKIENLFMGNSFGIVPTLDKQHMRGVVRTKVDD 365

  Fly   439 CHLLRVDKEHFNRILRDVEANTLRLQEHGKDVLV-----LERVAKQRGQHSAFKYTVMSGTPAKM 498
            |..:.:.::.:.|||..||.||.:::|.|:.|:|     |:|...::|      :.|:..||.::
  Rat   366 CQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKG------HIVIKATPERL 424

  Fly   499 LEHLLETRLGQSVGGMDP-FLDDFLLTHIVFMPV-VQLVDELANYFHCDAHEDAQT--------- 552
            :.||:|.   .|:  :|| :::|||||:..|:.. :.:..:|..:|..|:..|..|
  Rat   425 IMHLIEE---HSI--VDPTYIEDFLLTYRTFLETPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNN 484

  Fly   553 --------PEDREYIINFKK-----------RVIQF----MQKW--------------------- 573
                    |....::..|:|           |::..    ..||
  Rat   485 HFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLTGG 549

  Fly   574 ----------------------------VMAVRHAAFEEPSVCDFIE------------------ 592
                                        :|.|....||..:....:|
  Rat   550 SEKGFGVFVEVVEPGTKAADAGLKRGDQIMEVNGQNFENITFAKALEILRNNTHLALTVKTNIFV 614

  Fly   593 --DLAAEVEAD-------PDLNEETSIVHNVLTQMARYQEDRNQNAGQKWK-------------- 634
              :|.:..|.:       |.:.|:.|..|::.......::...:...:|.|
  Rat   615 FKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEQVPQEKGNKKIKANTVSGGRNKIRKI 679

  Fly   635 --------LPPNGQPICLFSGN-----------------------------ATPSKTVIRPD--- 659
                    |||.     |||..                             ::.|..:::|.
  Rat   680 LDKTRFSILPPK-----LFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSM 739

  Fly   660 --------------DDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVLVEVK 710
                          .|.:.||:.||...|.:.....|||:.:...|..:..|....:...|.||.
  Rat   740 LDFSNPSAVGFYYIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTYSLCEVS 804

  Fly   711 SNGERSVFK-----DNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGVDIDLEI--LST 768
            ...| .|.|     |....:...:.||||.:  :|::::..|...:::......:..|.:  |||
  Rat   805 VTPE-GVIKQRRLPDQFSKLADRIQLNGRYY--LKNNMETETLCSDEDAQELLKESQLSMLQLST 866

  Fly   769 KELAYHITLFEWDLFWAVHEYELLYHTF------GRHHFGKITANLDVFLRRF----NEVQYWIV 823
            .|:|..:::.::|||..:...|.:...|      |..|           |::|    |:..:|:.
  Rat   867 IEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTH-----------LKQFEDIVNQETFWVA 920

  Fly   824 TELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIF 888
            ||::|..:..||:.:::.|||:|.:|:|.:|.|:.||::.||:...|:||:.||||:|||:.|..
  Rat   921 TEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEKHL 985

  Fly   889 QEFEALIDPSRNHRAYRVFVG--KLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQ 951
            |:.:.|.|||||...||..:.  .:|||:||..|::.|||||.||||.:.:||||||||:.|:|:
  Rat   986 QDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAK 1050

  Fly   952 TMRTI----------------RFCRSRSLG-LEPPSPKS-----EG------------------- 975
            .:|.|                |..|.|||| |...|..|     :|
  Rat  1051 EIRHIIRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLY 1115

  Fly   976 -------EVRSYISSFRVIDNQRVLTAMSQKVEP 1002
                   :|:.|:||..:...:.....||.:.||
  Rat  1116 EDAQMARKVKQYLSSLDIDTEEEKFQMMSLQWEP 1149

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	CAP_ED	36..146	CDD:237999	24/109 (22%)
		DEP_Epac	199..330	CDD:239884	21/133 (16%)
		CAP_ED	352..463	CDD:237999	34/110 (31%)
		RasGEF_N	489..594	CDD:459873	31/207 (15%)
		Ubl1_cv_Nsp3_N-like	652..>722	CDD:475130	20/91 (22%)
		RasGEF	766..1002	CDD:214539	96/295 (33%)
Rapgef6	XP_017452748.1	CAP_ED	37..>82	CDD:469590	12/44 (27%)
		CAP_ED	280..380	CDD:237999	28/100 (28%)
		RasGEFN	412..525	CDD:214571	25/123 (20%)
		PDZ_RapGEF2_RapGEF6-like	529..611	CDD:467237	5/81 (6%)
		RA_PDZ-GEF1	756..838	CDD:340483	22/84 (26%)
		RasGEF	862..1086	CDD:238087	87/234 (37%)