Protein Alignment Epac and Rapgef4

DIOPT Version :10

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: NP_001094112.1
• Gene: Rapgef4 / 252857
• RGDID: 621886
• Length: 1011
• Species: Rattus norvegicus
• Alignment Length: 1031
• Identity: 503/1031 - (48%)
• Similarity: 683/1031 - (66%)
• Gaps: 64/1031 - (6%)

  Fly     4 EWITAMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGR 68
            |||..:||||.:|:..||::|..||:.|:...|...:.|:|:.:|.:||:||||:||||.|:.|.
  Rat    14 EWIACLDKRPLERSSEDVDIIFTRLKGVKAFERFHPNLLRQICLCSYYENLEKGITLFRQGDIGT 78

  Fly    69 FWYAVLGGSLEVRY---HAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRV 130
            .|||||.|||:|:.   .:|.|     |||:|.||:|..||||:|.:.||.:|:||:...||||:
  Rat    79 NWYAVLAGSLDVKVSETSSHQD-----AVTICTLGIGTAFGESILDNTPRHATIVTRENSELLRI 138

  Fly   131 EQQDFRLIWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKR--PLSPDHP--NPALPITET 191
            ||:||:.:|||.::.|..:     |...:|.....:.....|.|.  ||...|.  .||..||:.
  Rat   139 EQEDFKALWEKYRQYMAGL-----LAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPAHTITKV 198

  Fly   192 PSPAMNRMGWALRTLLVADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPIVHTRAQAAGMWQ 256
            ||..:.|.|..||..:::.....::|||...|..|:|..|||||||::..:..||:|.||.||||
  Rat   199 PSEKILRAGKILRIAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQ 263

  Fly   257 ALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGAANEHIREALSALFQRGPD 321
            .|||:|||.||::|:.|:||...|||..||.   ..|.:|..|:....:|.:::.:..|.|.|||
  Rat   264 VLLEDGVLNHVDQERHFQDKYLFYRFLDDEH---EDAPLPTEEEKKECDEELQDTMLLLSQMGPD 325

  Fly   322 ATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDE 386
            |.:|||||||..:||:::||::::||:||.|||||||::||||:.:.:||:||:.||:|||||:|
  Rat   326 AHMRMILRKPPGQRTADDLEIIYDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEE 390

  Fly   387 GRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNR 451
            |.||||:|||||:|||:|||.|.||..|||||||||:|||||||:|||:|:|||.||||||.|||
  Rat   391 GTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNR 455

  Fly   452 ILRDVEANTLRLQEHGKDVLVLERV------AKQRGQHSAFKYTVMSGTPAKMLEHLLET-RLGQ 509
            |||||||||:||:||.:||||||:|      :.|.......|||||||||.|:|||.||| ||..
  Rat   456 ILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEP 520

  Fly   510 SVG-GMDPFLDDFLLTHIVFMPVVQLVDELANYFHCDAHEDAQTPEDR-EYIINFKKRVIQFMQK 572
            |:. ..|..|:||::.|.||||..||...|..::|  |.....|.::| :|.:|.|:|||:.:.:
  Rat   521 SLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYH--AQPSQGTEQERMDYALNNKRRVIRLVLQ 583

  Fly   573 WVMAVRHAAFEEPSVCDFIEDLAAEVEADPDL----NEETSIVHNVLTQMARYQEDRNQNAGQKW 633
            |.........|:.....|:|:....|..|..:    .|:.:.:...:.|::   ||.
  Rat   584 WAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMVAFKEQLAELEKTVKQIS---EDA-------- 637

  Fly   634 KLPPNGQPICLFSGNA----TPSKTVIRPDDDIIFRVYCADHTYCTLRFPMHTTAELIKACAADK 694
            |.|.....:.|...|.    ...:..||..|:::|:|||.||||.|:|.|:..:.:.:.:..|||
  Rat   638 KAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYTTIRVPVAASVKEVISAVADK 702

  Fly   695 LQLNRGPEDLVLVEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGV 759
            |....|   |::|::.|.||:.|.|.||||:.|.|::|||||...::..|:||.|.|||.||.|.
  Rat   703 LGSGEG---LIIVKMNSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTTGT 764

  Fly   760 DIDLEILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITANLDVFLRRFNEVQYWIVT 824
            ....|::|:|:|||.:|.::|:||..|||.||:|||||||:|.|.|||||:|||||||:|:|:||
  Rat   765 VGTFELMSSKDLAYQMTTYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVT 829

  Fly   825 ELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQ 889
            |:.....|||||.|::||||:||:||||:|||:|||:||||||:|||||..||||:||||:|.:.
  Rat   830 EICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYA 894

  Fly   890 EFEALIDPSRNHRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMR 954
            |||:|:||||||||||:...||:||||||||||:|||||.||||||.:|.|||||||.|:|.|.|
  Rat   895 EFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTAR 959

  Fly   955 TIRFCRSRSLGLEPPSP------KSEGEVRSYISSFRVIDNQRVLTAMSQKVEPTR 1004
            |:|:.||     :|.:|      |:..:||||:....||||||.|:.||.::||.|
  Rat   960 TVRYYRS-----QPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRR 1010

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	CAP_ED	36..146	CDD:237999	55/112 (49%)
		DEP_Epac	199..330	CDD:239884	56/130 (43%)
		CAP_ED	352..463	CDD:237999	83/110 (75%)
		RasGEF_N	489..594	CDD:459873	43/107 (40%)
		Ubl1_cv_Nsp3_N-like	652..>722	CDD:475130	26/69 (38%)
		RasGEF	766..1002	CDD:214539	151/241 (63%)
Rapgef4	NP_001094112.1	CAP_ED	43..158	CDD:237999	56/124 (45%)
		DEP_Epac	206..334	CDD:239884	56/130 (43%)
		CAP_ED	356..467	CDD:237999	83/110 (75%)
		RasGEF_N	499..605	CDD:459873	43/107 (40%)
		RasGEF	768..1009	CDD:214539	153/245 (62%)