DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and Rapgef5

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	NP_001360918.1	Gene:	Rapgef5 / 217944	MGIID:	2444365	Length:	879	Species:	Mus musculus

Alignment Length:	931	Identity:	311/931 - (33%)
Similarity:	472/931 - (50%)	Gaps:	157/931 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly   161 PSVQATAAATSPTKRPLSPDHPNPALPITETPS----------PAMNRMGWAL---------RTL 206
            |::.:.|.|..|.:|     .|:...|..|.|:          ||:.|.|..|         |||
Mouse    15 PALASAAPADGPLRR-----SPSAREPEREPPAASLRPRLRDLPALLRSGLTLRRKRSAAGARTL 74

  Fly   207 ---------------LVADNSSC----------------LKDR-KVSGKLIRKCAPGTELVDWLV 239
                           :..:||||                :||| .:.|...|.|. |:||||||:
Mouse    75 SRRISNPYLEHSPSQIYGENSSCAGRALRNIIILQAADLVKDRVNLKGFYRRSCV-GSELVDWLL 138

  Fly   240 NLSPIVHTRAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGAA 304
            ...|.|..|:.|.|:||.||:.|:::.|::...|:|....|:|..||           ...|...
Mouse   139 EHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQFSSDE-----------CSYLYCE 192

  Fly   305 NEHIREALSALFQRGPDATLRMILRKP--------SHERTSEELELVFEELVHIAALSHLSTSIK 361
            .|...|     :|:|....|.::...|        ||::| |:.|...:|:     |:.|:::::
Mouse   193 FEREEE-----WQKGVKLLLELVHLIPARAGICDLSHQKT-EDSEESSDEI-----LARLTSAVQ 246

  Fly   362 RELSSIFVFEAHAQAGTILFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDA 426
            |||:::...:|...|    ..|.||....::.:..:.:......|.:..|:..||.|::.|::.
Mouse   247 RELAAVIALKARKSA----IEQDDENADKHVTVTEANNGPDPQAGVMCKLQERDDIGRIELVHK- 306

  Fly   427 PRAATIVLKENNCHLLRVDKEHFNRI-LRDVEANTLRLQEHGKDVLVLERVAK------QRGQHS 484
                   |...||..|:.:|:...:: .:|.|...::::|.|:.||||::||.      .....:
Mouse   307 -------LARENCQFLQTEKKESEKLEQQDDEVTMVQVKEQGQSVLVLKKVASCGPAPTSGSAEN 364

  Fly   485 AFKYTVMSGTPAKMLEHLL-----------ETRLGQSVGGMDPFLDDFLLTHIVFMPVVQLVDEL 538
            ..:|.|:||||.|:|||||           ||         :..|||||||:.|||....|...|
Mouse   365 DARYVVVSGTPEKILEHLLNDLHLAEVQHKET---------ETLLDDFLLTYTVFMTTDDLCQAL 420

  Fly   539 ANYFHCDAHEDAQTPEDREYIINFKKRVIQFMQKWVMAVRHAAFEEPSVCDFIEDLAAEVEAD-- 601
            ..::....:   |..|:...:...|::|:..:.:|:...:....|:.....|::.:...|..|
Mouse   421 LRHYSAKKY---QGEEENSDVPCRKRKVLHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVLDDVY 482

  Fly   602 --PDLNEETSIVHNVLTQMARYQED------RNQNAGQKWKLPPNGQPICLFSGNATPSKTVIRP 658
              |.|.:|......:|....|:..|      :|:....::.|..|     ......|.::|
Mouse   483 EYPILEKELKEFQKILGVYRRHTVDEYSPQKKNKALFHQFSLKEN-----WLQHRGTVAET---- 538

  Fly   659 DDDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVLVEVKSNGERSVFKDNDV 723
             ::|...||..:|:|.:::..:.:||:.|....|:|||  |..|||.||.:..:||:..|:.||:
Mouse   539 -EEIFCHVYITEHSYISVKAKVSSTAQEILKVVAEKLQ--RAEEDLALVAITFSGEKHEFQPNDL 600

  Fly   724 SIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGVDIDLEIL--STKELAYHITLFEWDLFWAV 786
            :|...|..:||::|..||..|.|..|.|.|   |.....:.||  :|.:||..:..|:|.||.::
Mouse   601 AISKSLEASGRIYVYRKDLADTLNPLAENE---ESQQRSMRILGMNTWDLALELMSFDWSLFNSI 662

  Fly   787 HEYELLYHTFGRHHFGKITANLDVFLRRFNEVQYWIVTELVSTPSLSKRVGLVRKFIKLAAYCKE 851
            ||.||:|.||.|...|:.|.||.:.|:|.||||.|:.||::....|.|||.||:||||:||:||.
Mouse   663 HEQELIYFTFSRQGNGENTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFIKIAAHCKA 727

  Fly   852 YQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEALIDPSRNHRAYRVFVGKLQPPLI 916
            .||||:|||:||||:..:||||.|||||||.||:|:|.|.|:|.|||.||:|||....|::||.|
Mouse   728 QQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKI 792

  Fly   917 PFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRTIRFCRSRSLGLEPPSPKSEGEVRSYI 981
            |||||||||:||.||||||.||.||||||:||:|.|:||:|.||:...|.: .|||.:.|::||:
Mouse   793 PFMPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGSD-VSPKEQQELKSYV 856

  Fly   982 SSFRVIDNQRVLTAMSQKVEP 1002
            :...|||:|:.|..:|.::||
Mouse   857 NHLYVIDSQQALFELSHRLEP 877

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736
		CAP_ED	36..146	CDD:237999
		DEP_Epac	199..330	CDD:239884	45/171 (26%)
		CAP_ED	352..463	CDD:237999	23/111 (21%)
		Crp	373..525	CDD:223736	45/169 (27%)
		RasGEF_N	489..594	CDD:279012	32/115 (28%)
		UBQ	665..>714	CDD:294102	17/48 (35%)
		RasGEF	766..1002	CDD:214539	131/237 (55%)
Rapgef5	NP_001360918.1	DEP_Epac	98..221	CDD:239884	37/139 (27%)
		RasGEFN	367..498	CDD:214571	38/142 (27%)
		RasGEF	639..878	CDD:214539	134/240 (56%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	66	1.000	Domainoid score	I9884
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	O	PTHR23113
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2054
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	10	9.850