DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and epac-1

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	NP_499256.2	Gene:	epac-1 / 176432	WormBaseID:	WBGene00004255	Length:	1038	Species:	Caenorhabditis elegans

Alignment Length:	1076	Identity:	367/1076 - (34%)
Similarity:	555/1076 - (51%)	Gaps:	143/1076 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 VELISCRLRRVEPLCRLPGSAL-QQLAMCGFYED-LEKGVTLFRAGEQGRFWYAVLGGSLEVRYH 83
            :|.|..|:||:.||.....:.| ..|:...|..| :::||.||...|...:||.:|.|.::: |.
Worm     1 MERIVSRVRRLSPLHTFSDALLISLLSESDFQPDSIQQGVVLFEKDEPTEYWYLLLSGEVQL-YS 64

  Fly    84 AHADADGKSAVTL-CNLGVGATFGESVLHDLPRDSTVVTKTTCELLRVEQQDFRLIWEKNKE--- 144
            .....|.....|| |    ||.||:  |..|....:.:.....:|:|:.|..|..::.|:.:
Worm    65 KTYTGDFNHLKTLRC----GALFGD--LSTLTHSCSCLVTRPAQLIRIAQNHFLSVYNKHGDHLQ 123

  Fly   145 ----LMNDIFT-----------NCKLKNG----------FGPSVQATAAATSPTKRPLSPDHPNP 184
                :|:||.|           :..|.||          ..||...:.:......:.:.|..||.
Worm   124 PFIIIMHDILTDETPSDPIHPHSSGLFNGQRSMDLISTEINPSEIVSVSTNGMLSKMILPSIPNQ 188

  Fly   185 ALPITETPSPAMNRM----------------------------GWALRTLLVADNSSCLKDRKVS 221
                .|.|   |||:                            |..|...::.||...::|....
Worm   189 ----REKP---MNRVVVNQQKNEERNFIEFHNPTGIEKQIRDSGGILHRKMLTDNHQVIRDITTE 246

  Fly   222 GKLIRKCAPGTELVDWLVNLSPIVHT------RAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLY 280
            ...::.|..|.|::|||:.|.....|      |.|.:.:||.||..|:::|::.|..|.||...|
Worm   247 HTRVQNCMIGAEMIDWLLTLFVSTSTTCSSLSRIQMSAIWQVLLNNGLISHIDGEHQFLDKTNSY 311

  Fly   281 -----RFRIDEEGGTAAAGVPQAEDLGAANEHIREALSALFQRGPDATLRMILRKPSHERTSEEL 340
                 :||             ....:..:.|.:.::::.|....|:....||:.||..||:.|||
Worm   312 YRWVQQFR-------------SRNKVAPSIEEVSKSITLLSSVAPETLFLMIVSKPGFERSPEEL 363

  Fly   341 ELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDEGRSWYILLKGSVDVVIHGK 405
            |:|:|||..|.|||||||.:||:||:....|.:..||:::|.||:.|..|||:|||:|:|.::||
Worm   364 EVVYEELTFIKALSHLSTMVKRQLSNFVKVEQYVHAGSVVFRQGEIGVYWYIVLKGAVEVNVNGK 428

  Fly   406 GTVATLKTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNRILRDVEANTLRLQEHGKDV 470
             .|..|:.|||||||||:||.|||||||..|::...|.|||.|||:||..|||||:||:::|:||
Worm   429 -IVCLLREGDDFGKLALVNDLPRAATIVTYEDDSMFLVVDKHHFNQILHQVEANTVRLKDYGEDV 492

  Fly   471 LVLERVAKQRGQHSAFK----------YTVMSGTPAKMLEHLLETR---LGQSVGGMDPFLDDFL 522
            ||||:|...||  :|.:          |:||:|...|:||::||||   ||..:..:|.|::||:
Worm   493 LVLEKVDIPRG--AALENSNSCNFNCGYSVMAGKAEKILEYVLETRIDALGDDISELDVFVEDFI 555

  Fly   523 LTHIVFMPVVQLVDELANYFH-------CDAHEDAQTPEDREYIINFKKRVIQFMQKWVMAVRHA 580
            |||..|||...:.:.|.:|:.       .|:..|:.|.|     :..|:||:||:..|...:|..
Worm   556 LTHDAFMPDNTVCNFLKSYYFRTPYRATRDSITDSCTEE-----VRCKRRVVQFVYVWCSLLRVN 615

  Fly   581 AFEEPSVCDFIEDLAAEVEADPDLNEETSIVHNVLTQMARYQEDRNQNAGQKWKLPPNGQPICLF 645
            .|..|....|:|:|...|..|   .:....:.::||::...:..|     :..:|.....|..:.
Worm   616 FFLNPVTNSFVEELFCHVIDD---RKRLGGMEDILTRIGSIRSTR-----ENMQLVLARHPAIVL 672

  Fly   646 SGNATPSKT--VIRPDDDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVLVE 708
            ......:.|  .:.|.|.....:|.||.|...|...:..|||.|  |...:.:::...|.|.|||
Worm   673 DCGVLSAHTPCPVLPSDVCNQIIYLADTTCFVLPIRVDKTAEEI--CELSRRRMSFSAEPLNLVE 735

  Fly   709 VKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGVDID-LEILSTKELA 772
            ||||||:.:|..||.:|||.||||.:|:|..::.:..|..:::|..||...... |.::.::|||
Worm   736 VKSNGEKLIFSPNDRAIPTVLSLNSKLYVVNREEIPLLVPMEDQNGPTPSSHSSILHLIDSQELA 800

  Fly   773 YHITLFEWDLFWAVHEYELLYHTFGRHHFG-KITANLDVFLRRFNEVQYWIVTELVSTPSLSKRV 836
            :.:.||...|..:....||||...||..|. .:..|||:.:|||||||:|..||::.... ..|:
Worm   801 HQLFLFHLQLLRSTDSNELLYQVIGRESFPLSMPFNLDLLVRRFNEVQHWSTTEILLATE-ENRM 864

  Fly   837 GLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEALIDPSRNH 901
            .:::|||.:|...:||::|...||:.:|||:.::|||..||.|:|....|.|.|.|.|:||:|||
Worm   865 EILKKFISIATIAREYRDLLTVFAITLGLSHTSISRLTLTWSKLPPASLKTFSELENLLDPTRNH 929

  Fly   902 RAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRTIRFCRSRSLGL 966
            |.||:.|.|:..|.|||:||:|||:.|.|:|||:..:||||||||||.|:..|:.|.|:|:   :
Worm   930 RMYRLLVSKMSSPYIPFVPLILKDLMFIHQGNKSFYNGLVNFEKMHMFAKIFRSFRQCKSQ---M 991

  Fly   967 EPPSPKSEGEVRSYISSFRVIDNQRVLTAMSQKVEP 1002
            :..:.....|.:|.|.:.||||||:.|..:|.::||
Worm   992 DNGAEHEFIEPQSLIRNLRVIDNQKKLMQLSYEIEP 1027

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	33/127 (26%)
		CAP_ED	36..146	CDD:237999	29/119 (24%)
		DEP_Epac	199..330	CDD:239884	33/169 (20%)
		CAP_ED	352..463	CDD:237999	63/110 (57%)
		Crp	373..525	CDD:223736	82/164 (50%)
		RasGEF_N	489..594	CDD:279012	39/114 (34%)
		UBQ	665..>714	CDD:294102	19/48 (40%)
		RasGEF	766..1002	CDD:214539	101/236 (43%)
epac-1	NP_499256.2	Crp	8..>120	CDD:223736	33/118 (28%)
		cNMP	19..129	CDD:197516	29/116 (25%)
		DEP_Epac	224..353	CDD:239884	33/141 (23%)
		CAP_ED	375..485	CDD:237999	63/110 (57%)
		Crp	397..>477	CDD:223736	46/80 (58%)
		RasGEFN	516..654	CDD:214571	45/145 (31%)
		RasGEF	791..1029	CDD:214539	104/241 (43%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160166483
Domainoid	1	1.000	169	1.000	Domainoid score	I2293
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4451
Inparanoid	1	1.050	535	1.000	Inparanoid score	I652
Isobase	1	0.950	-	0.878984	Normalized mean entropy	S3294
OMA	1	1.010	-	-		QHG49338
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	1	1.000	-	-		oto19650
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	LDO	PTHR23113
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R9235
SonicParanoid	1	1.000	-	-		X1712
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	18	17.750