Protein Alignment Epac and PDZ-GEF

DIOPT Version :10

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: XP_061514825.1
• Gene: PDZ-GEF / 1280224
• VectorBaseID: AGAMI1_013726
• Length: 1705
• Species: Anopheles gambiae
• Alignment Length: 1123
• Identity: 239/1123 - (21%)
• Similarity: 408/1123 - (36%)
• Gaps: 391/1123 - (34%)

  Fly   240 NLSPIVHTRAQAAGMWQALLEEG-VLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGA 303
            |:..:.||.:....::..|.::| .|:|.:..........:....:|.:....:..|....|  :
Mosquito   148 NMHRLKHTSSLTTSIYPELYDKGNRLSHSSDTSQADTMTSVTSSSLDSDEVDLSGLVESVVD--S 210

  Fly   304 ANEHIREALSALFQRGPDATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIF 368
            ..|.|.|::.:|..|.   |:|..|.|...:||.|::|::.|....:.|.::::.:::|.|.::.
Mosquito   211 DEEDIAESIDSLSVRD---TVRECLEKDPTDRTVEDIEILLEFTQKLKAFTNMTFAVRRALCAVM 272

  Fly   369 VFEAHAQAGTILFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIV 433
            ||....:|||::.|.|:|..||.:|:.|.|::. ||.|.|..|..||.||.:..::.......:.
Mosquito   273 VFAVVEKAGTVVMNDGEELDSWSVLINGHVEIE-HGTGEVEYLHYGDSFGIMPTMDKLYHRGVMR 336

  Fly   434 LKENNCHLLRVDKEHFNRILRDVEANTLRLQEHGKDVLVLERVAKQRGQHSAFK-YTVMSGTPAK 497
            .|.::|..:.:.:..:.||....|.|..:::|.|:.|:|.| :....|::.:.| |.|:.||..:
Mosquito   337 TKCDDCQFVCITQTDYYRIQHQGEDNIRKIEEDGQVVMVTE-LRNSTGENGSRKGYVVIRGTVER 400

  Fly   498 MLEHLLE-TRLGQSVGGMDP-FLDDFLLTHIVFM--PVVQLVDELANYFHCD---AHEDAQTPE- 554
            ::..|:| |.|      .|| :::|||||...|:  | :.:..:|..:|:.|   :.||..:.:
Mosquito   401 LMHQLVEDTTL------TDPNYVEDFLLTCRTFINNP-IDISKQLLKWFNVDGGGSSEDGGSTDL 458

  Fly   555 -----------DREYIIN-------FKKRVIQFMQKWVMAVRH-AAFE-EPSVCDFIEDLAAEVE 599
                       ||...::       ...||.:.:..||.  .| ..|: :|.:.:|:|...:.:|
Mosquito   459 VDNATAATVSIDRSSHLSSGTLDSVLCDRVTRVVLLWVN--NHFTDFDTDPQMMEFLEIFESALE 521

  Fly   600 ------------------------------ADPDLNEETS------------------------- 609
                                          .|.|||.:.|
Mosquito   522 KRSMYEQLRLLHVYAQHNARERNVTLARSSRDEDLNFQISGGPGGIFITRVEPKTKAYEAGLKRG 586

  Fly   610 ----------------------------------------------------------------- 609
                                                                             
Mosquito   587 DQILEVNGQSFEHVTCARALEILMGTTHLSITVKSNLWAFKGMLSSADGDSSKLKIDLKKGLRTE 651

  Fly   610 ----------------------------------------------------------------- 609
                                                                             
Mosquito   652 LLQSQKSLDLVDKVGGGLLPAPQFLMPLSVRDIEYGRKDSGASTPSAQMNNNKGGSFMTLGGKKR 716

  Fly   610 -----IVHNVLTQMARYQEDR----------------------------NQNAGQKW-------- 633
                 |..|:|.:.:.:|:|.                            |.|.|..:
Mosquito   717 IQKALIKMNLLPKNSVFQDDNINNNHNNGGGGGSNNSSSSSGGGGANNGNSNNGSSYEGGSDRSS 781

  Fly   634 ------------------------KLPPNG----------QPICLFSGNAT----PSKT------ 654
                                    :.||..          .|.......:|    |:||
Mosquito   782 GISVNFSSQASSISTSSITTADSDETPPTSAYQSNPDLRENPSTPAKAQSTEAIVPAKTGGTMYY 846

  Fly   655 -VIRPDD--DIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVLVEVKSNGERS 716
             .:|..|  :.|.:||.:|.|...|.....|||..:...|..:..::....:..|.|| |.||..
Mosquito   847 EELRASDFPEHILKVYKSDQTCKYLLVHKETTAHEVVMLALQEFGIHDPSSNFSLCEV-SVGEGG 910

  Fly   717 VFKDNDVSIPTGL-SLNGRLFVSVKDHLDALTQLQEQECPTEGV-------DIDLEI-------- 765
            :.|..  .:|..| :|..|:.:|.:.:|           .|.|:       |:..|:
Mosquito   911 MIKQR--RLPDQLQNLAERIGLSSRYYL-----------KTNGITETLVPDDVAPELIRESAVHF 962

  Fly   766 --LSTKELAYHITLFEWDLFWAVHEYELLYHTFG-RHHFGKITANLDVFLRRFNEVQYWIVTELV 827
              |:..|||..:||.::.:|..:...|.:...|. :..:||  ..|..|....|...:|:|||:.
Mosquito   963 LQLNANELAIQLTLQDFSIFRQIESTEYIDDLFNLKSRYGK--PMLVRFAELVNREMFWVVTEVC 1025

  Fly   828 STPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFE 892
            |..::.:|..::::|||:|.:|||.:|.|:.||:|.||.:.|||||:|||||:|||::|:|.:.:
Mosquito  1026 SEHNMMRRCKIIKQFIKIARHCKECKNFNSLFAIVSGLGHAAVSRLRQTWEKLPSKYQKLFNDLQ 1090

  Fly   893 ALIDPSRNHRAYRVFVG---KLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMR 954
            .|:|||||...||..:.   ..|.|:|||.|::.||:||.|.||.|.:|.|:||||:.|:::.:|
Mosquito  1091 DLMDPSRNMSKYRQLIQTELNAQQPVIPFYPVVKKDLTFIHLGNDTKIDSLINFEKLRMISKEIR 1155

  Fly   955 T-IRFCR-------------------------------SRSLGLEPPSPKSEGEVRSYISSFRVI 987
            | :..|.                               |.:|.|.||:|.....|.|..::|  :
Mosquito  1156 TLLHMCNSPYDILTMLEYKCQPPSSAMLALNQMSVPSGSGNLMLAPPAPPPPPPVTSVAAAF--V 1218

  Fly   988 DNQRVLTAMSQKVEPTRK 1005
            .:|.|.........|..|
Mosquito  1219 HSQTVKRRKKSTAAPNPK 1236

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	CAP_ED	36..146	CDD:237999
		DEP_Epac	199..330	CDD:239884	18/90 (20%)
		CAP_ED	352..463	CDD:237999	31/110 (28%)
		RasGEF_N	489..594	CDD:459873	32/132 (24%)
		Ubl1_cv_Nsp3_N-like	652..>722	CDD:475130	21/78 (27%)
		RasGEF	766..1002	CDD:214539	92/271 (34%)
PDZ-GEF	XP_061514825.1	None