Protein Alignment Epac and RAPGEF4

DIOPT Version :10

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: NP_008954.2
• Gene: RAPGEF4 / 11069
• HGNCID: 16626
• Length: 1011
• Species: Homo sapiens
• Alignment Length: 1043
• Identity: 511/1043 - (48%)
• Similarity: 685/1043 - (65%)
• Gaps: 88/1043 - (8%)

  Fly     4 EWITAMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGR 68
            |||..:||||.:|:..||::|..||:.|:...:...:.|.|:.:||:||:||||:||||.|:.|.
Human    14 EWIACLDKRPLERSSEDVDIIFTRLKEVKAFEKFHPNLLHQICLCGYYENLEKGITLFRQGDIGT 78

  Fly    69 FWYAVLGGSLEVRY---HAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRV 130
            .|||||.|||:|:.   .:|.|     |||:|.||:|..||||:|.:.||.:|:||:.:.||||:
Human    79 NWYAVLAGSLDVKVSETSSHQD-----AVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRI 138

  Fly   131 EQQDFRLIWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKR--PLSPDHP--NPALPITET 191
            ||:||:.:|||.::.|..:     |...:|.....:.....|.|.  ||...|.  .||..||:.
Human   139 EQKDFKALWEKYRQYMAGL-----LAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKV 198

  Fly   192 PSPAMNRMGWALRTLLVADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPIVHTRAQAAGMWQ 256
            ||..:.|.|..||..:::.....::|||...|..|:|..|||||||::..:|.||:|.||.||||
Human   199 PSEKILRAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQ 263

  Fly   257 ALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGAANEHIREALSALFQRGPD 321
            .|||:|||.||::|..|:||...|||..||.   ..|.:|..|:....:|.:::.:..|.|.|||
Human   264 VLLEDGVLNHVDQEHHFQDKYLFYRFLDDEH---EDAPLPTEEEKKECDEELQDTMLLLSQMGPD 325

  Fly   322 ATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDE 386
            |.:|||||||..:||.::||:::|||:||.|||||||::||||:.:.:||:||:.||:|||||:|
Human   326 AHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEE 390

  Fly   387 GRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNR 451
            |.||||:|||||:|||:|||.|.||..|||||||||:|||||||:|||:|:|||.||||||.|||
Human   391 GTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNR 455

  Fly   452 ILRDVEANTLRLQEHGKDVLVLERV------AKQRGQHSAFKYTVMSGTPAKMLEHLLET-RLGQ 509
            |||||||||:||:||.:||||||:|      :.|.......|||||||||.|:|||.||| ||..
Human   456 ILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEA 520

  Fly   510 SVG-GMDPFLDDFLLTHIVFMPVVQLVDELANYFHCDAHEDAQTPEDREYIINFKKRVIQFMQKW 573
            ::. ..|..|:||::.|.||||..||...|..::|....:..: .|..:|.:|.|:|||:.:.:|
Human   521 TLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTE-QEKMDYALNNKRRVIRLVLQW 584

  Fly   574 VMA---------VRHAAFEE--PSVCDFIEDLAAEVEADPDLNEETSIV----------HNVLTQ 617
            ...         |..|..||  .||.|....:||..|..|:|.:....:          |.||.|
Human   585 AAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVLLQ 649

  Fly   618 MARYQEDRNQNAGQKWKLPPNGQPICLFSGNATPSKTVIRPDDDIIFRVYCADHTYCTLRFPMHT 682
            .....::|.|..          ||              ||..|:::|:|||.||||.|:|.|:.|
Human   650 QFNTGDERAQKR----------QP--------------IRGSDEVLFKVYCMDHTYTTIRVPVAT 690

  Fly   683 TAELIKACAADKLQLNRGPEDLVLVEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALT 747
            :.:.:.:..||||....|   |::|::.|.||:.|.|.||||:.|.|::|||||...::..|:||
Human   691 SVKEVISAVADKLGSGEG---LIIVKMSSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLT 752

  Fly   748 QLQEQECPTEGVDIDLEILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITANLDVFL 812
            .|.|||.||.|.....|::|:|:|||.:|:::|:||..|||.||:|||||||:|.|.|||||:||
Human   753 PLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFL 817

  Fly   813 RRFNEVQYWIVTELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTW 877
            |||||:|:|:|||:.....|||||.|::||||:||:||||:|||:|||:||||||:|||||..||
Human   818 RRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTW 882

  Fly   878 EKIPSKFRKIFQEFEALIDPSRNHRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVN 942
            ||:||||:|.:.|||:|:||||||||||:.|.||:||||||||||:|||||.||||||.:|.|||
Human   883 EKLPSKFKKFYAEFESLMDPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVN 947

  Fly   943 FEKMHMMAQTMRTIRFCRSRSLGLEPPSP------KSEGEVRSYISSFRVIDNQRVLTAMSQKVE 1001
            ||||.|:|.|.||:|:.||     :|.:|      |:..:||||:....||||||.|:.||.::|
Human   948 FEKMRMIANTARTVRYYRS-----QPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLE 1007

  Fly  1002 PTR 1004
            |.|
Human  1008 PRR 1010

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	CAP_ED	36..146	CDD:237999	55/112 (49%)
		DEP_Epac	199..330	CDD:239884	57/130 (44%)
		CAP_ED	352..463	CDD:237999	83/110 (75%)
		RasGEF_N	489..594	CDD:459873	44/117 (38%)
		Ubl1_cv_Nsp3_N-like	652..>722	CDD:475130	27/69 (39%)
		RasGEF	766..1002	CDD:214539	152/241 (63%)
RAPGEF4	NP_008954.2	CAP_ED	43..158	CDD:237999	56/124 (45%)
		DEP_Epac	206..334	CDD:239884	57/130 (44%)
		CAP_ED	356..467	CDD:237999	83/110 (75%)
		RasGEF_N	499..605	CDD:459873	40/106 (38%)
		RasGEF	768..1009	CDD:214539	154/245 (63%)