DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and RAPGEF3

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	XP_011536054.1	Gene:	RAPGEF3 / 10411	HGNCID:	16629	Length:	938	Species:	Homo sapiens

Alignment Length:	972	Identity:	378/972 - (38%)
Similarity:	536/972 - (55%)	Gaps:	131/972 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly   101 VGATFGESVLHDLPRDSTVVTKTTCELLRVEQQDFRLIWEKNKE-----LMNDIFTNCKLKNGFG 160
            |||      |.|:..:.|::......:.|.....::|:.|..:.     |.....||.:....|.
Human    28 VGA------LPDVVPEGTLLNMVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFS 86

  Fly   161 PSVQATAAATSPTKRPLSPDHPNPALPITETPSPAMNRMGWALRTLLVADNSSCLKDRKVSGKLI 225
            .|::..:     |:|.|                    |.|..|...|:|...:.::|||...:|.
Human    87 ESLEQAS-----TERVL--------------------RAGRQLHRHLLATCPNLIRDRKYHLRLY 126

  Fly   226 RKCAPGTELVDWLVNLSPIVHTRAQAAGMWQALLEEGVLAHVNKEQP---------------FKD 275
            |:|..|.||||.::.|...||:|:|..|:.|.||:||.|.|.:...|               |:|
Human   127 RQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHASISSPPPRLGLCPPVKHDWAFQD 191

  Fly   276 K-CFLYRFRIDEEGGTAAAGVPQAEDLGAANEHIREALSALFQRGPDATLRMILRKPSHERTSEE 339
            : ...|||...|         |:........|.:.||::.|.||||||.|.:.||||..:||.||
Human   192 RDAQFYRFPGPE---------PEPVRTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEE 247

  Fly   340 LELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDEGRSWYILLKGSVDVVIHG 404
            |:|:||||:||.|::|||.|:||||:::.:||.|::|||:||:|||:|.||||:.||||:||.||
Human   248 LDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHG 312

  Fly   405 KGTVATLKTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNRILRDVEANTLRLQEHGKD 469
            ||.|.||..|||||:|||:|||||||||:|:|:|||.|||||:.||||::||||.|:||:||||.
Human   313 KGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKV 377

  Fly   470 VLVLERVAKQRGQH-----SAFKYTVMSGTPAKMLEHLLETRLGQSVGGMDP---FLDDFLLTHI 526
            ||||||.::..|..     ...:||||||||.|:||.|||. :|......||   ||.||||||.
Human   378 VLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEA-MGPDSSAHDPTETFLSDFLLTHR 441

  Fly   527 VFMPVVQLVDELANYFHCDAHEDAQTPEDREYIINFKKRVIQFMQKWVMAVRHAAFEEPSVCDFI 591
            ||||..||...|.::||.:....:: .|...|:.|.::::::.:.:||.........:|....|:
Human   442 VFMPSAQLCAALLHHFHVEPAGGSE-QERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFL 505

  Fly   592 EDLAAEVEADPDLNEETSIVHNVLTQMARYQEDRN--------QNAGQKWK-------LPPNGQP 641
            :.|:..|..|..|:       |:|.:  ::.|.|.        .||..:.|       ||...:|
Human   506 QKLSDLVGRDTRLS-------NLLRE--QWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEP 561

  Fly   642 ICLFSGNATPSKTVIRPDDDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVL 706
            :       ..|...|:..|.:.:.:...||:..||:.|:..:...:.|..|.:....:|.   ||
Human   562 L-------PGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQ---VL 616

  Fly   707 VEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGVDIDLEILSTKEL 771
            |:|.|.|:....:.:...:.|.|.||.||||.....:..|....:|..||.|....|:::|.|:|
Human   617 VKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDL 681

  Fly   772 AYHITLFEWDLFWAVHEYELLYHTFGRHHFGKI-TANLDVFLRRFNEVQYWIVTELVSTPSLSKR 835
            |..:|..:|.||.::|:.||:::..|..|...: ||||:.|:|||||:|||:.|||...|....|
Human   682 AGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPR 746

  Fly   836 VGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEALIDPSRN 900
            ..|:||||||||:.||.:|||:||||:.||||.|:|||..|||::|.|.||::...|.|:|||.|
Human   747 AQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWN 811

  Fly   901 HRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRTIRFCRSRS-L 964
            ||.||:.:.||.||:|||||||||||||.||||.|.::.|:|||||.|||:..|.:..|||.: :
Human   812 HRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPV 876

  Fly   965 GLEP------------------------PSPKSEGEVRSYISSFRVIDNQRVLTAMSQKVEP 1002
            .|.|                        .|.:|.....:|:...:||||||.|:.:|:::||
Human   877 PLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP 938

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	9/49 (18%)
		CAP_ED	36..146	CDD:237999	9/49 (18%)
		DEP_Epac	199..330	CDD:239884	48/146 (33%)
		CAP_ED	352..463	CDD:237999	74/110 (67%)
		Crp	373..525	CDD:223736	99/159 (62%)
		RasGEF_N	489..594	CDD:279012	40/107 (37%)
		UBQ	665..>714	CDD:294102	13/48 (27%)
		RasGEF	766..1002	CDD:214539	122/261 (47%)
RAPGEF3	XP_011536054.1	DEP_Epac	100..238	CDD:239884	48/146 (33%)
		CAP_ED	262..371	CDD:237999	73/108 (68%)
		Crp	283..>361	CDD:223736	56/77 (73%)
		REM	407..526	CDD:100121	41/129 (32%)
		RasGEF	673..905	CDD:238087	112/231 (48%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	136	1.000	Domainoid score	I4930
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG49338
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	LDO	PTHR23113
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R9235
SonicParanoid	1	1.000	-	-		X1712
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.820