DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Epac and rapgef3

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	XP_005162410.1	Gene:	rapgef3 / 101886094	ZFINID:	ZDB-GENE-090312-38	Length:	885	Species:	Danio rerio

Alignment Length:	909	Identity:	385/909 - (42%)
Similarity:	542/909 - (59%)	Gaps:	100/909 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly   152 NCKLKNGF-GPSVQATA-------AATSPTKRPLSPDHPNPALPITETPSPAMNRMGWALRTLLV 208
            :.|.||.. .|:|..:|       ..:|..|.|| ||..:....|.:..|..:.:....:.:.|:
Zfish    17 SAKKKNRLESPAVMCSADTLLRSTLRSSLQKTPL-PDAVDTQDTIKQFLSDRILKAARVVYSTLM 80

  Fly   209 ADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPIVHTRAQAAGMWQALLEEGVLAHVNKEQPF 273
            ..|...::|||...|..|:|..|.||||||:.|:....:|:||.||||.|::||:|.||.:|..|
Zfish    81 EGNPGLIRDRKHHLKTYRQCCSGKELVDWLMKLNDCFQSRSQAVGMWQVLVDEGILGHVKQELNF 145

  Fly   274 KDK-CFLYRFRIDEEGGTAAAGVPQAE-DLG-------AANEHIREALSALFQRGPDATLRMILR 329
            .|| ...|||.             :|| ||.       :.::.::|:||.|.|.||||.|.||||
Zfish   146 HDKDTQFYRFM-------------EAEFDLNHTTNEKDSKDDELQESLSLLIQMGPDALLTMILR 197

  Fly   330 KPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDEGRSWYILL 394
            |...:||.|:||:::|||:|:.|::|||||:::||:|:.|||:||:|||:||:|||:|.||||:.
Zfish   198 KCPSQRTPEDLEVIYEELLHVKAVAHLSTSVRKELASVLVFESHAKAGTVLFSQGDKGTSWYIIW 262

  Fly   395 KGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNRILRDVEAN 459
            :|||:|:.||||.|.||..|||||:|||:|||||||||:|:|:|||.|||||:.|.|||:|||||
Zfish   263 RGSVNVITHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFIRILKDVEAN 327

  Fly   460 TLRLQEHGKDVLVLERVAKQRGQHSAFKYTVMSGTPAKMLEHLLETRLGQSVGG--MDPFLDDFL 522
            |:||:||||.|||||:.:.| ...|..|||||||||.|:|||:||....::.|.  :||.:.|||
Zfish   328 TVRLEEHGKVVLVLEKSSAQ-DTPSCNKYTVMSGTPEKILEHMLEAIKLETNGADFIDPCVTDFL 391

  Fly   523 LTHIVFMPVVQLVDELANYFHCDAHEDAQTPEDREYIINFKKRVIQFMQKWVMAVRHAAFEEPSV 587
            |||.||||..||...|.:::..:..|.:.. |...|.:|.|::|::.:..||........::|..
Zfish   392 LTHPVFMPCSQLCAALQHHYQAEPSEGSDL-EKAAYALNTKQKVVKLVCHWVALFALLLRDDPVA 455

  Fly   588 CDFIEDLAAEVEADPDLNEETSIVHNVLTQMARYQ-EDRNQNAGQKWKLPPNG--------QPIC 643
            .:|:|.....|.||           :.|:.|.|.| .||     :|.|:..||        |...
Zfish   456 SEFLEKFREGVMAD-----------SRLSSMLREQLRDR-----RKTKIMENGCHTLTKLNQKFD 504

  Fly   644 LFSGNATPSKTV--IRPDDDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPE-DLV 705
            .||....|...:  |:..|.:::.::..||...|:..|:..:...|.....|       |: :.|
Zfish   505 WFSAYEEPVGKLRSIKAQDKVLYEIFKPDHKAVTVMLPVDASVNDILTTLVD-------PDRNYV 562

  Fly   706 LVEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGVDIDLEILSTKE 770
            ||::.|:|:|...|....::...|.:|.:||:.....::.||..:||..|.:.....||.:.:|:
Zfish   563 LVKMNSSGDRVQLKLETTAVSASLGVNEKLFLCTSSQVEQLTPDKEQLGPEKSTMDTLEQICSKD 627

  Fly   771 LAYHITLFEWDLFWAVHEYELLYHTFGRHHF-GKITANLDVFLRRFNEVQYWIVTELVSTPSLSK 834
            :|...|.::|:||.|:||.||:|:.|||..| |..||||:.|:|||||||||:||||.....|.|
Zfish   628 MASQHTSYDWELFMAMHEVELVYYVFGREKFPGSTTANLERFVRRFNEVQYWVVTELCLCEDLGK 692

  Fly   835 RVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEALIDPSR 899
            |..|::||||:|...||.:|||:||||:.||||.||.||.:|||::|:|.::|:..:|.|:||||
Zfish   693 RAILLKKFIKMAVVLKEQKNLNSFFAVMFGLSNSAVQRLNKTWERLPNKTKRIYCAYERLMDPSR 757

  Fly   900 NHRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRTIRFCRSRSL 964
            ||||||:.:.||.||.|||||||||||||.|||||...|.|||||||.|:|:|::|:|.|||:.
Zfish   758 NHRAYRLTIAKLSPPYIPFMPLLLKDMTFIHEGNKNYTDKLVNFEKMRMIARTVKTVRDCRSQP- 821

  Fly   965 GLEPPSP---------------------------KSEGEVRSYISSFRVIDNQRVLTAMSQKVE 1001
             ..|.||                           :|...:|.||.:.:|||||:.||.:|:.:|
Zfish   822 -YVPSSPQKGLTERMFLDAQAIRISTYSDQSLNLRSATSIRQYIQNLKVIDNQKKLTQLSRALE 884

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736
		CAP_ED	36..146	CDD:237999
		DEP_Epac	199..330	CDD:239884	53/139 (38%)
		CAP_ED	352..463	CDD:237999	75/110 (68%)
		Crp	373..525	CDD:223736	98/153 (64%)
		RasGEF_N	489..594	CDD:279012	40/106 (38%)
		UBQ	665..>714	CDD:294102	11/49 (22%)
		RasGEF	766..1002	CDD:214539	133/264 (50%)
rapgef3	XP_005162410.1	DEP_Epac	71..198	CDD:239884	53/139 (38%)
		CAP_ED	222..331	CDD:237999	74/108 (69%)
		Crp	241..>321	CDD:223736	55/79 (70%)
		RasGEFN	353..485	CDD:214571	51/148 (34%)
		RasGEF	620..884	CDD:214539	134/265 (51%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	135	1.000	Domainoid score	I4923
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG49338
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	LDO	PTHR23113
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1712
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	11	10.790