Protein Alignment Epac and rapgef5a

DIOPT Version :10

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: NP_001429693.1
• Gene: rapgef5a / 100331732
• ZFINID: ZDB-GENE-030131-7681
• Length: 874
• Species: Danio rerio
• Alignment Length: 894
• Identity: 287/894 - (32%)
• Similarity: 435/894 - (48%)
• Gaps: 139/894 - (15%)

  Fly   174 KRPLSPDH-PNPALPITETP---SPAMNRMGWALRTLLVADNSSCLKDRKVSGKLIRKCAPGTEL 234
            |:..|||. |.|:..:|..|   |.:....|.|||...::......||:....::||:...|.||
Zfish    53 KKSQSPDTIPGPSSGVTPKPKTCSQSFPCAGRALRNAFLSHGPYPAKDKIHLARIIRRSYVGVEL 117

  Fly   235 VDWLVNLSPIVHTRAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAE 299
            |.||:.....|..|..|:.:||.|||.|:|..|::...|:|....|:|..:|   ..|.|.....
Zfish   118 VQWLLEQCVAVQCRLMASRVWQVLLELGILHSVDQRLVFEDSNTYYQFSFEE---CDAQGCEFRN 179

  Fly   300 DLGAANEHIREALSALFQRGPDATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKREL 364
            :     |..:..:..|.|..|....|::  .|..|....:.: |..|::.:.||..|:::::.||
Zfish   180 E-----EEWQNGVRLLLQLVPYVQFRVV--SPPEEDPERKRD-VCSEILQMKALERLTSTVQNEL 236

  Fly   365 SSIFVFEA---------------------------HAQAGTILFNQGDEGRSWYILLKGSVDVVI 402
            ::....:|                           ..|||
Zfish   237 AAALARKAKKSMSEDESSETSDSSPQEPPKPTEDKSKQAG------------------------- 276

  Fly   403 HGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNCHL----LRVDKEHFNRILRDVEANTLRL 463
                 |..|:..|||.:|.::....:.....|:..|..|    .:...|...|:.         |
Zfish   277 -----VCALRAPDDFSRLEMVQRLAKDGYRFLQNQNYRLPDRSAQAQGEGVVRVC---------L 327

  Fly   464 QEHGKDVLVLERV---------AKQRGQHSAFKYTVMSGTPAKMLEHLL-ETRLGQSVGG----- 513
            :|.|:|||||:||         |..:...|..:|.|:||||.|:||||| :.||.:..|.
Zfish   328 KERGQDVLVLQRVPSDPASPQTAGVKDDDSDKRYVVVSGTPHKILEHLLSDLRLDEHQGATEGKE 392

  Fly   514 MDPFLDDFLLTHIVFMPVVQLVDELANYF---HCDAHEDAQTPEDREYIINFKKRVIQFMQKWVM 575
            .:..|||||||::|||...:|...|..::   .|...|:......|      |::|:..:.:|..
Zfish   393 AEMLLDDFLLTYLVFMSTSELCQALLGHYCTKRCRGKEEGNEALYR------KRKVLHLVSQWSS 451

  Fly   576 AVRHAAFEEPSVCDFIEDLAAEVEAD----PDLNEETSIVHNVLTQMARYQED------RNQNAG 630
            ..:....||.:|..|::.|...|..|    |.|.::......:|.:  |:..|      :|:...
Zfish   452 LYKDFLREEENVKLFMKTLYRYVLEDVYEFPTLEKDLKEFQKLLRR--RHTVDDYSPHQKNKALY 514

  Fly   631 QKWKLPPNGQPICLFSGNATPSKTVIRPDDDIIFRVYCADHTYCTLRF-PMHTTAELIKACAADK 694
            |:..|..|.     .|..|:|.::     .|::.|||.:..:|.::|. |.....||:...:.
Zfish   515 QQLSLKENS-----VSLRASPVES-----RDVMCRVYVSCDSYLSMRVKPAVVAQELLHIVSQ-- 567

  Fly   695 LQLNRGPEDLVLVEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEG- 758
             :::|..:|::|:....:||:.|.:.:|......|...|||....:|..:.|..|  .|||..|
Zfish   568 -RMDRCEDDMMLLAQTYSGEKRVLQPHDSIYSESLVAPGRLIACRRDLSEILPPL--TECPDLGQ 629

  Fly   759 VDIDLEILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITANLDVFLRRFNEVQYWIV 823
            ..:.|..::|.::|..:|.|:|:||.::||.||:::||.|......|..|:..|:|.||||.|::
Zfish   630 KPVRLLGINTWDVAVALTNFDWNLFNSIHEQELIFYTFSRQASSGHTVALEFLLQRCNEVQQWVM 694

  Fly   824 TELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIF 888
            :|::..|||||||.|::||||:||:||..:|||:.||::|||:..|||||.|||||:|.||:|:|
Zfish   695 SEVLLCPSLSKRVQLLKKFIKIAAHCKAQRNLNSSFAIIMGLNTAAVSRLNQTWEKVPGKFKKLF 759

  Fly   889 QEFEALIDPSRNHRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTM 953
            .|.|.|.|||.||:|||....|::||.||||||||||:||.||||||..|.||||||:||:|.|:
Zfish   760 SELELLTDPSMNHKAYRDAFKKMKPPKIPFMPLLLKDITFIHEGNKTFHDNLVNFEKLHMIADTV 824

  Fly   954 RTIRFCRSRSLGLEPPSPKSEGEVRSYISSFRVIDNQRVLTAMSQKVEP 1002
            |.||.|:....|.| .|.....||||.:....:||||:.|..:|.::||
Zfish   825 RLIRHCQMDQTGNE-LSAVDSAEVRSSVHYLHIIDNQQTLFELSHRLEP 872

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	CAP_ED	36..146	CDD:237999
		DEP_Epac	199..330	CDD:239884	37/130 (28%)
		CAP_ED	352..463	CDD:237999	20/141 (14%)
		RasGEF_N	489..594	CDD:459873	36/113 (32%)
		Ubl1_cv_Nsp3_N-like	652..>722	CDD:475130	15/70 (21%)
		RasGEF	766..1002	CDD:214539	123/235 (52%)
rapgef5a	NP_001429693.1	None