DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Epac and rapgef4b

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	NP_001108368.1	Gene:	rapgef4b / 100141331	ZFINID:	ZDB-GENE-080219-4	Length:	427	Species:	Danio rerio

Alignment Length:	437	Identity:	195/437 - (44%)
Similarity:	273/437 - (62%)	Gaps:	28/437 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 EWITAMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGR 68
            |||..:||||.:|:..||::|..||:.|:...:...:.|||:.||||||.||||:||:|.|:.|.
Zfish    15 EWIICLDKRPAERSGEDVDIILARLKGVKAFEKFHPNLLQQICMCGFYEYLEKGITLYRQGDIGT 79

  Fly    69 FWYAVLGGSLEVRYHAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRVEQQ 133
            .|||||.|||:|:  ....|:.:.|||:|.||||..||||:|.:.||.:|:|::...||||:||:
Zfish    80 SWYAVLSGSLDVK--VSETANHQDAVTICTLGVGTAFGESILDNTPRHATIVSRENSELLRIEQR 142

  Fly   134 DFRLIWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKRPLSPDHPN-PALPITETPSPAMN 197
            :|:.:|||.::.|..:     |...:|...........|.|..||.|..| .:..:.:.||..:.
Zfish   143 EFKTLWEKYRQCMAGL-----LAPPYGVMDSGATNGRMPDKDNLSNDPLNFMSKNLNKVPSEKIL 202

  Fly   198 RMGWALRTLLVADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPIVHTRAQAAGMWQALLEEG 262
            |....||..::|.....::|||...|..|:|..||||||||:..|..||:||.|.||||.|||||
Zfish   203 RAEKVLRNAILARAPHMIRDRKYHLKTYRQCCVGTELVDWLLQQSSCVHSRAHAVGMWQVLLEEG 267

  Fly   263 VLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGAANEHIREALSALFQRGPDATLRMI 327
            ||.||:.|..|:||...|||..|||   ..|.:|..::...|.|.::|.|..|.|.||||.:|||
Zfish   268 VLNHVDHELNFQDKYLFYRFLDDEE---EDAVLPSDDEKREAEEELQETLLFLSQIGPDAHMRMI 329

  Fly   328 LRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDEGRSWYI 392
            ||||..:||:|:||::::||:||.|:||||.::||||:.:.:||:||:|||:|||||:||.||||
Zfish   330 LRKPPGQRTAEDLEIIYDELLHIKAVSHLSNTVKRELAGVLIFESHAKAGTVLFNQGEEGTSWYI 394

  Fly   393 LLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNC 439
            :.||||:|||:||.....|:                 ||:.::.:.|
Zfish   395 IQKGSVNVVIYGKPMTLVLR-----------------ATLCVRMHMC 424

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	57/117 (49%)
		CAP_ED	36..146	CDD:237999	55/109 (50%)
		DEP_Epac	199..330	CDD:239884	62/130 (48%)
		CAP_ED	352..463	CDD:237999	40/88 (45%)
		Crp	373..525	CDD:223736	29/67 (43%)
		RasGEF_N	489..594	CDD:279012
		UBQ	665..>714	CDD:294102
		RasGEF	766..1002	CDD:214539
rapgef4b	NP_001108368.1	CAP_ED	44..153	CDD:237999	55/110 (50%)
		Crp	65..>153	CDD:223736	46/89 (52%)
		DEP_Epac	204..332	CDD:239884	62/130 (48%)
		CAP_ED	356..>406	CDD:237999	33/49 (67%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	275	1.000	Domainoid score	I1719
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	941	1.000	Inparanoid score	I334
OMA	1	1.010	-	-		QHG49338
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	1	1.000	-	-		otm25598
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	O	PTHR23113
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1712
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.870