Protein Alignment SCAP and Scap

DIOPT Version :10

• Sequence 1: NP_788277.1
• Gene: SCAP / 35529
• FlyBaseID: FBgn0033052
• Length: 1276
• Species: Drosophila melanogaster
• Sequence 2: NP_001094436.1
• Gene: Scap / 301024
• RGDID: 1309378
• Length: 1276
• Species: Rattus norvegicus
• Alignment Length: 1429
• Identity: 430/1429 - (30%)
• Similarity: 633/1429 - (44%)
• Gaps: 370/1429 - (25%)

  Fly    55 LPASVSHFYYKHGLFLSSYPTCASSIAFMAILLSCYPLINIPLPGTIPTKIVVPYETGSGSLSWH 119
            |...:|..:|.|||..:|||........:.||..||||:.:|||||.|.:...|.:
  Rat     7 LREKISQAFYNHGLLCASYPIPIILFTGLCILACCYPLLKLPLPGTGPVEFSTPVK--------- 62

  Fly   120 SLNTSSTTPQEPHPSGEPWPPEPQVLNSSTTDRSPPPLLPWAQSSPAFFYVQQITLRTSVLPWTE 184
              ..|.......|..||| ..:|:                |...:|. .|:|||.:::||.||..
  Rat    63 --GYSPPPADSDHKQGEP-SEQPE----------------WYVGAPV-AYIQQIFVKSSVSPWHR 107

  Fly   185 GMQLMDAFRAPLHEVFKLLEIVRNH--QSSENKRTLEHNCLHVDNVKRGTHGQLDQIFPEYGCLL 247
            .:..:|.||:||...|:|:|.:|||  :.|...::||..||.|.::..|.. :|..:.||:||||
  Rat   108 NLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGTKSLEEVCLQVTDLLPGLR-KLRSLLPEHGCLL 171

  Fly   248 LSPANLWTQNSQNFTRDTNILNTIFQYH--NLQKSKVSAAEMLFGLPMQDTGFKRYPLRARSRII 310
            |||.|.|..:.:.|..|.:|:.||.|:.  .||.| .:..::|||:|.:.:|...|   .|.|::
  Rat   172 LSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTS-ATLKDLLFGVPGKYSGVSLY---TRKRMV 232

  Fly   311 QYALTLFLKHNDMEYLDTLKEKLLRHYPPLPLASASAEEPTTITYIFYPGEYRMWELVPYTVAFM 375
            .|.:||..:....::|.:|:.:|:..:|. |..|..||   .:.::.:..|..:.||:|....::
  Rat   233 SYTITLVFQRYHAKFLSSLRARLMLLHPS-PNCSLRAE---NLVHVHFKEEIGIAELIPLVTTYI 293

  Fly   376 LVFAYVYFSVRKIDVFRSRFLLALCSVITTAGSLAMSLGLCFFFGLTISLQSKDIFPYLVILVGL 440
            ::|||:|||.||||:.:|::.|||.:|:|...||.||:|||..||||.:|...:||||||:::||
  Rat   294 ILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFPYLVVVIGL 358

  Fly   441 ENSLVITKSVVSMDETFDVKIRVAQALSKEGWHISKTLLTEITILTIGLATFVPVIQEFCIFAIV 505
            ||.||:||||||.....:||:|:||.||.|.|.|.|.:.||:.|:.||..|.||.|||||:||:|
  Rat   359 ENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNVATELGIILIGYFTLVPAIQEFCLFAVV 423

  Fly   506 GLLSDFMLQMLLFSTILAMNIKRTEYTAEAKHLPKMLLSCTQGA---GRQDFRFFGAAPALPPFV 567
            ||:|||.||||.|:|:|:::|:|.|.....|.||..  ||...|   ||
  Rat   424 GLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPE--SCLPSAKPVGR---------------- 470

  Fly   568 PGTFQRS----QSHPKLCFADPASVSDRTSLVNGHSSPEQRIPKRIKIVNFWARTRFFQRAFMIW 628
            |..::|.    .|.|......|:|..:            .|:|||::::.|.||||..||..|..
  Rat   471 PARYERQLAVRPSTPHTITLQPSSFRN------------LRLPKRLRVIYFLARTRLAQRLIMAG 523

  Fly   629 MIVWICSIVYNS-----GYLEQLFSMQSNGTMTATLELQRRLQAGRGAVSSFFEGWQADGQRATS 688
            .:|||..:||..     .||....:.||              ..|.|::...            .
  Rat   524 TVVWIGILVYTDPAGLRTYLAAQVTEQS--------------PLGEGSLGPM------------P 562

  Fly   689 APSG--------SGFST-PIKAPLAIDINET-----------AEEMMRLRYPSF-------DLNY 726
            .|||        ..||. |..|| .:..|:|           ||.:...|.|..       :|..
  Rat   563 VPSGVLPASHPDPAFSIFPPDAP-KLPENQTLPGELPEHAVPAEGVQDSRAPEVTWGPEDEELWR 626

  Fly   727 FLSNFHWSTIMKQYNISLSGHYVTLLPTIRLSHAIAPELATLLRNPQEQLQQNFQWKALAAALDP 791
            .||..||.|:...|||:|:..|::|||.|.::..:.|..|...|:||:   ....|    |..:|
  Rat   627 KLSFRHWPTLFNYYNITLAKRYISLLPVIPVTLHLNPREALEGRHPQD---GRTAW----APPEP 684

  Fly   792 L----------------DFNDDDVRRESPMVMAEGLPLVPKSPMEIFFAILLCCISIFVLCYTMV 840
            |                ...|..:.:.:.:.:|.|:.||          :||.|:
  Rat   685 LPAGLWETGPKGPGGTQTHGDITLYKVAALGLAAGIVLV----------LLLLCL---------- 729

  Fly   841 VFYRCICTRNYAE---WRSSWHESEAP---YKQTEQILEGVPTQIAGHKHRIECLVSDGAYIISC 899
              ||.:|.|||.:   ........|.|   |.......|.||..:.||...||||.|||..::||
  Rat   730 --YRVLCPRNYGQPGGGAGRRRRGELPCDDYGYAPPETEIVPLVLRGHLMDIECLASDGMLLVSC 792

  Fly   900 CLKGQIRVWDARSGEQLTSISR----------------------SD------------------- 923
            ||.||:.||||::|:.||.|.|                      ||
  Rat   793 CLAGQVCVWDAQTGDCLTRIPRPGPRRDSCGGGAFEAQENWERLSDGGKASPEEPGDSPPLRRRP 857

  Fly   924 ------------------------IQISQQRT------------------------------DGQ 934
                                    :|:..:.|                              :|.
  Rat   858 RGPPPPSLFGDQPDLTCLIDTNFSVQLPPEPTQPEPRHRAGCGRSRDSGYDFSRLVQRVYQEEGL 922

  Fly   935 TLVRKLAVSP----------------------------------VWCLDYFDNLIAVGCANGRVE 965
            ..|...|:.|                                  :|.|:...:||.||.::||:|
  Rat   923 AAVHMSALRPPSPGPPLPQASQEEGTAPEKGSPPLAWAPSTAGSIWSLELQGSLIVVGRSSGRLE 987

  Fly   966 LWESPAGLLKCAYQEDAKRNQGITHIHLNGDRVIVARLNGRLDFYRLETYYKGKQIDWGFTSAYR 1030
            :|::..|:|.|:.:|   .:.|||.:.....|::.|||||.|||:.|||:.....:.      :|
  Rat   988 VWDAIEGVLCCSNEE---ISSGITALVFLDRRIVAARLNGSLDFFSLETHTSLSPLQ------FR 1043

  Fly  1031 RTHVRTGSTGSLGLMLQQQRCQQEASQKTTKEEMKITLEGVRLAHQQPITCMQVVNDMVFTGSQD 1095
            .|..|..|..|          ...:|..|....:..|   |..|||:|||.::.....:.|||||
  Rat  1044 GTPGRGSSPSS----------PVYSSSNTVACHLTHT---VPCAHQKPITALRAAAGRLVTGSQD 1095

  Fly  1096 HTLKVYCLNKSDVEYTLHGHCGPVTCLFVDRWQPGTGGSGSQDGLLCVWDLFTGACMYNIQAHDG 1160
            |||:|:.|..|...:||.||.|.:|.:::|  |.....||.|||.:|:||:.||:.:.:..||.|
  Rat  1096 HTLRVFRLEDSCCLFTLQGHSGAITTVYID--QTMVLASGGQDGAICLWDVLTGSRVSHTFAHRG 1158

  Fly  1161 AVSCLACAPSYVISLGTDERICVWERFQGNLLTTINIS-NAYSSLLMLTPSLLVTSKMGSLIVWD 1224
            .|:.|.|..|.|||.|.|:.|.:|:|..|..|.:|... ...:||.:::.:||||...|.:..||
  Rat  1159 DVTSLTCTTSCVISSGLDDFINIWDRSTGIKLYSIQQDLGCGASLGVISDNLLVTGGQGCVSFWD 1223

  Fly  1225 VRTG--------------QPAREVKLDFANLQLCPKIMMLACDSVVCDYGNEIRVVRFPIVADK 1274
            :..|              ||||:             |::|...::||::|:|:.:|..|.|.:|
  Rat  1224 LNYGDLLQTVYLGKNSEAQPARQ-------------ILVLDNAAIVCNFGSELSLVYVPSVLEK 1274

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SCAP	NP_788277.1	Sterol-sensing	390..543	CDD:463544	81/152 (53%)
		WD40	878..1185	CDD:475233	118/435 (27%)
		WD40 repeat	887..942	CDD:293791	27/149 (18%)
		WD40 repeat	945..983	CDD:293791	14/37 (38%)
		WD40 repeat	989..1070	CDD:293791	21/80 (26%)
		WD40 repeat	1079..1114	CDD:293791	15/34 (44%)
		WD40 repeat	1120..1157	CDD:293791	13/36 (36%)
		WD40 repeat	1162..1196	CDD:293791	14/33 (42%)
		WD40 repeat	1201..1233	CDD:293791	13/45 (29%)
Scap	NP_001094436.1	2A060601	<11..>466	CDD:273337	192/494 (39%)
		Loop-1. /evidence=ECO:0000250|UniProtKB:P97260	46..284		82/275 (30%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	60..81		6/32 (19%)
		Sterol-sensing	308..452	CDD:463544	78/143 (55%)
		ER export signal. /evidence=ECO:0000250|UniProtKB:P97260	447..452		1/4 (25%)
		Loop-7. /evidence=ECO:0000250|UniProtKB:P97260	535..710		45/208 (22%)
		Interaction with SREBF2. /evidence=ECO:0000250|UniProtKB:P97260	731..1276		158/581 (27%)
		WD40	771..802	CDD:197651	16/30 (53%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	834..903		4/68 (6%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	928..958		2/29 (7%)
		WD40 repeat	1008..1044	CDD:293791	13/41 (32%)
		WD40	1075..>1232	CDD:475233	62/158 (39%)
		WD40 repeat	1079..1113	CDD:293791	14/33 (42%)
		WD40 repeat	1120..1153	CDD:293791	13/34 (38%)
		WD40 repeat	1160..1194	CDD:293791	14/33 (42%)