Protein Alignment SCAP and Scap

DIOPT Version :10

• Sequence 1: NP_788277.1
• Gene: SCAP / 35529
• FlyBaseID: FBgn0033052
• Length: 1276
• Species: Drosophila melanogaster
• Sequence 2: NP_001001144.2
• Gene: Scap / 235623
• MGIID: 2135958
• Length: 1276
• Species: Mus musculus
• Alignment Length: 1429
• Identity: 432/1429 - (30%)
• Similarity: 637/1429 - (44%)
• Gaps: 370/1429 - (25%)

  Fly    55 LPASVSHFYYKHGLFLSSYPTCASSIAFMAILLSCYPLINIPLPGTIPTKIVVPYETGSGSLSWH 119
            |...:|..:|.|||..:|||........:.||..||||:.:|||||.|.:...|.:
Mouse     7 LREKISQAFYNHGLLCASYPIPIILFTGLCILACCYPLLKLPLPGTGPVEFSTPVK--------- 62

  Fly   120 SLNTSSTTPQEPHPSGEPWPPEPQVLNSSTTDRSPPPLLPWAQSSPAFFYVQQITLRTSVLPWTE 184
              ..|.......|..||| ..:|:                |...:|. .|:|||.:::||.||..
Mouse    63 --GYSPPPADSDHKQGEP-SEQPE----------------WYVGAPV-AYIQQIFVKSSVSPWHR 107

  Fly   185 GMQLMDAFRAPLHEVFKLLEIVRNH--QSSENKRTLEHNCLHVDNVKRGTHGQLDQIFPEYGCLL 247
            .:..:|.||:||...|:|:|.:|||  :.|...::||..||.|.::..|.. :|..:.||:||||
Mouse   108 NLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGTKSLEEVCLQVTDLLPGLR-KLRSLLPEHGCLL 171

  Fly   248 LSPANLWTQNSQNFTRDTNILNTIFQYH--NLQKSKVSAAEMLFGLPMQDTGFKRYPLRARSRII 310
            |||.|.|..:.:.|..|.:|:.||.|:.  .||.| .:..::|||:|.:.:|...|   .|.|::
Mouse   172 LSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTS-ATLKDLLFGVPGKYSGVSLY---TRKRMV 232

  Fly   311 QYALTLFLKHNDMEYLDTLKEKLLRHYPPLPLASASAEEPTTITYIFYPGEYRMWELVPYTVAFM 375
            .|.:||..:....::|.:|:.:|:..:|. |..|..||   .:.::.:..|..:.||:|....::
Mouse   233 SYTITLVFQRYHAKFLSSLRARLMLLHPS-PNCSLRAE---NLVHVHFKEEIGIAELIPLVTTYI 293

  Fly   376 LVFAYVYFSVRKIDVFRSRFLLALCSVITTAGSLAMSLGLCFFFGLTISLQSKDIFPYLVILVGL 440
            ::|||:|||.||||:.:|::.|||.:|:|...||.||:|||..||||.:|...:||||||:::||
Mouse   294 ILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFPYLVVVIGL 358

  Fly   441 ENSLVITKSVVSMDETFDVKIRVAQALSKEGWHISKTLLTEITILTIGLATFVPVIQEFCIFAIV 505
            ||.||:||||||.....:||:|:||.||.|.|.|.|...||:.|:.||..|.||.|||||:||:|
Mouse   359 ENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNAATELGIILIGYFTLVPAIQEFCLFAVV 423

  Fly   506 GLLSDFMLQMLLFSTILAMNIKRTEYTAEAKHLPKMLLSCTQGA---GRQDFRFFGAAPALPPFV 567
            ||:|||.||||.|:|:|:::|:|.|.....|.||..  ||...|   ||
Mouse   424 GLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPE--SCLPSAKPVGR---------------- 470

  Fly   568 PGTFQRSQ----SHPKLCFADPASVSDRTSLVNGHSSPEQRIPKRIKIVNFWARTRFFQRAFMIW 628
            |..::|.|    |.|......|:|..:            .|:|||::::.|.||||..||..|..
Mouse   471 PARYERQQAVRPSTPHTITLQPSSFRN------------LRLPKRLRVIYFLARTRLAQRLIMAG 523

  Fly   629 MIVWICSIVYNS-----GYLEQLFSMQSNGTMTATLELQRRLQAGRGAVSSFFEGWQADGQRATS 688
            .:|||..:||..     .||....:.||              ..|.|::...            .
Mouse   524 TVVWIGILVYTDPAGLRTYLAAQVTEQS--------------PLGEGSLGPM------------P 562

  Fly   689 APSG--------SGFST-PIKAPLAIDINET-----------AEEMMRLRYPSF-------DLNY 726
            .|||        ..||. |..|| .:..|:|           ||.:...|.|..       :|..
Mouse   563 VPSGVLPASHPDPAFSIFPPDAP-KLPENQTLPGELPEHAGPAEGVHDSRAPEVTWGPEDEELWR 626

  Fly   727 FLSNFHWSTIMKQYNISLSGHYVTLLPTIRLSHAIAPELATLLRNPQEQLQQNFQWKALAAALDP 791
            .||..||.|:...|||:|:..|::|||.|.::..:.|..|...|:||:       .::..|..:|
Mouse   627 KLSFRHWPTLFNYYNITLAKRYISLLPVIPVTLHLNPREALEGRHPQD-------GRSAWAPQEP 684

  Fly   792 L----------------DFNDDDVRRESPMVMAEGLPLVPKSPMEIFFAILLCCISIFVLCYTMV 840
            |                ...|..:.:.:.:.:|.|:.||          :||.|:
Mouse   685 LPAGLWESGPKGPGGTQTHGDITLYKVAALGLAAGIVLV----------LLLLCL---------- 729

  Fly   841 VFYRCICTRNYAE---WRSSWHESEAP---YKQTEQILEGVPTQIAGHKHRIECLVSDGAYIISC 899
              ||.:|.|||.:   ........|.|   |.......|.||..:.||...||||.|||..::||
Mouse   730 --YRVLCPRNYGQPGGGPGRRRRGELPCDDYGYAPPETEIVPLVLRGHLMDIECLASDGMLLVSC 792

  Fly   900 CLKGQIRVWDARSGEQLTSISR----------------------SD------------------- 923
            ||.||:.||||::|:.||.|.|                      ||
Mouse   793 CLAGQVCVWDAQTGDCLTRIPRPGPRRDSCGGGAFETQENWERLSDGGKASPEEPGDSPPLRRRP 857

  Fly   924 ------------------------IQISQQRT------------------------------DGQ 934
                                    :|:..:.|                              :|.
Mouse   858 RGPPPPSLFGDQPDLTCLIDTNFSVQLPPEPTQPEPRHRVGCGRSRDSGYDFSRLVQRVYQEEGL 922

  Fly   935 TLVRKLAVSP----------------------------------VWCLDYFDNLIAVGCANGRVE 965
            ..:|..|:.|                                  :|.|:...|||.||.::||:|
Mouse   923 AAMRMPALRPPSPGPPLPQASQEEGTAPEKGSPPLAWTPSTAGSIWSLELQGNLIVVGRSSGRLE 987

  Fly   966 LWESPAGLLKCAYQEDAKRNQGITHIHLNGDRVIVARLNGRLDFYRLETYYKGKQIDWGFTSAYR 1030
            :|::..|:|.|:.:|   .:.|||.:.....|::.|||||.|||:.|||:.....:.      :|
Mouse   988 VWDAIEGVLCCSNEE---ISSGITALVFLDRRIVAARLNGSLDFFSLETHTSLSPLQ------FR 1043

  Fly  1031 RTHVRTGSTGSLGLMLQQQRCQQEASQKTTKEEMKITLEGVRLAHQQPITCMQVVNDMVFTGSQD 1095
            .|..| ||:.|..:.         :|..|.......|   |..|||:|||.::.....:.|||||
Mouse  1044 GTPGR-GSSPSSSVY---------SSSNTVTCHRTHT---VPCAHQKPITALRAAAGRLVTGSQD 1095

  Fly  1096 HTLKVYCLNKSDVEYTLHGHCGPVTCLFVDRWQPGTGGSGSQDGLLCVWDLFTGACMYNIQAHDG 1160
            |||:|:.|:.|...:||.||.|.:|.:::|  |.....||.|||.:|:||:.||:.:....||.|
Mouse  1096 HTLRVFRLDDSCCLFTLKGHSGAITAVYID--QTMVLASGGQDGAICLWDVLTGSRVSQTFAHRG 1158

  Fly  1161 AVSCLACAPSYVISLGTDERICVWERFQGNLLTTINIS-NAYSSLLMLTPSLLVTSKMGSLIVWD 1224
            .|:.|.|..|.|||.|.|:.|.:|:|..|..|.:|... ...:||.:::.:||||...|.:..||
Mouse  1159 DVTSLTCTASCVISSGLDDFISIWDRSTGIKLYSIQQDLGCGASLGVISDNLLVTGGQGCVSFWD 1223

  Fly  1225 VRTG--------------QPAREVKLDFANLQLCPKIMMLACDSVVCDYGNEIRVVRFPIVADK 1274
            :..|              ||||:             |::|...::||::|:|:.:|..|.|.:|
Mouse  1224 LNYGDLLQTVYLGKNSEAQPARQ-------------ILVLDNAAIVCNFGSELSLVYVPSVLEK 1274

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SCAP	NP_788277.1	Sterol-sensing	390..543	CDD:463544	81/152 (53%)
		WD40	878..1185	CDD:475233	120/435 (28%)
		WD40 repeat	887..942	CDD:293791	27/149 (18%)
		WD40 repeat	945..983	CDD:293791	15/37 (41%)
		WD40 repeat	989..1070	CDD:293791	22/80 (28%)
		WD40 repeat	1079..1114	CDD:293791	15/34 (44%)
		WD40 repeat	1120..1157	CDD:293791	13/36 (36%)
		WD40 repeat	1162..1196	CDD:293791	14/33 (42%)
		WD40 repeat	1201..1233	CDD:293791	13/45 (29%)
Scap	NP_001001144.2	2A060601	<11..>466	CDD:273337	192/494 (39%)
		Loop-1. /evidence=ECO:0000250|UniProtKB:P97260	46..284		82/275 (30%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	60..81		6/32 (19%)
		Sterol-sensing	308..452	CDD:463544	78/143 (55%)
		ER export signal. /evidence=ECO:0000250|UniProtKB:P97260	447..452		1/4 (25%)
		Loop-7. /evidence=ECO:0000250|UniProtKB:P97260	535..710		44/208 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	669..698		6/35 (17%)
		Interaction with SREBF2. /evidence=ECO:0000250|UniProtKB:P97260	731..1276		160/581 (28%)
		WD40	771..802	CDD:197651	16/30 (53%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	834..903		4/68 (6%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	927..960		2/32 (6%)
		WD40	<952..1144	CDD:441893	72/215 (33%)
		WD40 repeat	957..998	CDD:293791	13/40 (33%)
		WD40 repeat	1008..1071	CDD:293791	22/81 (27%)
		WD40	1065..>1232	CDD:475233	65/171 (38%)
		WD40 repeat	1079..1113	CDD:293791	14/33 (42%)
		WD40 repeat	1120..1154	CDD:293791	13/35 (37%)
		WD40 repeat	1160..1194	CDD:293791	14/33 (42%)