DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment SCAP and SCAP

DIOPT Version :9

Sequence 1:	NP_788277.1	Gene:	SCAP / 35529	FlyBaseID:	FBgn0033052	Length:	1276	Species:	Drosophila melanogaster
Sequence 2:	NP_036367.2	Gene:	SCAP / 22937	HGNCID:	30634	Length:	1279	Species:	Homo sapiens

Alignment Length:	1441	Identity:	430/1441 - (29%)
Similarity:	634/1441 - (43%)	Gaps:	391/1441 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    55 LPASVSHFYYKHGLFLSSYPTCASSIAFMAILLSCYPLINIPLPGTIPTKIVVPYETGSGSLSWH 119
            |...:|..:|.|||..:|||..........||..||||:.:|||||.|.:.
Human     7 LREKISRAFYNHGLLCASYPIPIILFTGFCILACCYPLLKLPLPGTGPVEF-------------- 57

  Fly   120 SLNTSSTTPQEPHPSGEPWPPEPQVLNSSTTDRSPPPLLPWAQSSPAFFYVQQITLRTSVLPWTE 184
                  |||.:.:        .|..::|......|.....|...:|. .|||||.:::||.||.:
Human    58 ------TTPVKDY--------SPPPVDSDRKQGEPTEQPEWYVGAPV-AYVQQIFVKSSVFPWHK 107

  Fly   185 GMQLMDAFRAPLHEVFKLLEIVRNH--QSSENKRTLEHNCLHVDNVKRGTHGQLDQIFPEYGCLL 247
            .:..:|.||:||...|:|:|.:|||  :.|...|:||..||.|.::..|.. :|..:.||:||||
Human   108 NLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGIRSLEELCLQVTDLLPGLR-KLRNLLPEHGCLL 171

  Fly   248 LSPANLWTQNSQNFTRDTNILNTIFQYH--NLQKSKVSAAEMLFGLPMQDTGFKRYPLRARSRII 310
            |||.|.|..:.:.|..|.:|:.||.|:.  .||.| .:..::|||:|.:.:|...|   .|.|::
Human   172 LSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTS-ATLKDLLFGVPGKYSGVSLY---TRKRMV 232

  Fly   311 QYALTLFLKHNDMEYLDTLKEKLLRHYPPLPLASASAEEPTTITYIFYPGEYRMWELVPYTVAFM 375
            .|.:||..:|...::|.:|:.:|:..:|. |..|..||   ::.::.:..|..:.||:|....::
Human   233 SYTITLVFQHYHAKFLGSLRARLMLLHPS-PNCSLRAE---SLVHVHFKEEIGVAELIPLVTTYI 293

  Fly   376 LVFAYVYFSVRKIDVFRSRFLLALCSVITTAGSLAMSLGLCFFFGLTISLQSKDIFPYLVILVGL 440
            ::|||:|||.||||:.:|::.|||.:|:|...||.||:|||..||||.:|...:||||||:::||
Human   294 ILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFPYLVVVIGL 358

  Fly   441 ENSLVITKSVVSMDETFDVKIRVAQALSKEGWHISKTLLTEITILTIGLATFVPVIQEFCIFAIV 505
            ||.||:||||||.....:||:|:||.||.|.|.|.|.:.||:.|:.||..|.||.|||||:||:|
Human   359 ENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSIMKNMATELGIILIGYFTLVPAIQEFCLFAVV 423

  Fly   506 GLLSDFMLQMLLFSTILAMNIKRTEYTAEAKHLPKMLLSCTQGAGRQDFRFFGAAPALPPFVPGT 570
            ||:|||.||||.|:|:|:::|:|.|.....|.||..  :|...             |.|...|..
Human   424 GLVSDFFLQMLFFTTVLSIDIRRMELADLNKRLPPE--ACLPS-------------AKPVGQPTR 473

  Fly   571 FQRS----QSHPKLCFADPASVSDRTSLVNGHSSPEQRIPKRIKIVNFWARTRFFQRAFMIWMIV 631
            ::|.    .|.|......|:|..:            .|:|||:::|.|.||||..||..|...:|
Human   474 YERQLAVRPSTPHTITLQPSSFRN------------LRLPKRLRVVYFLARTRLAQRLIMAGTVV 526

  Fly   632 WICSIVYNS-----GYLEQLFSMQSNGTMTATLELQRRLQAGRGAVSSFFEGWQADGQRATSAPS 691
            ||..:||..     .||....:.||              ..|.||::..            ..||
Human   527 WIGILVYTDPAGLRNYLAAQVTEQS--------------PLGEGALAPM------------PVPS 565

  Fly   692 G--------SGFST-PIKAPLAIDINETA----------------------------EEMMRLRY 719
            |        ..||. |..|| .:..|:|:                            ||:.|.
Human   566 GMLPPSHPDPAFSIFPPDAP-KLPENQTSPGESPERGGPAEVVHDSPVPEVTWGPEDEELWRK-- 627

  Fly   720 PSFDLNYFLSNFHWSTIMKQYNISLSGHYVTLLPTIRLSHAIAPELATLLRNPQEQLQQNF---- 780
                    ||..||.|:...|||:|:..|::|||.|.:         ||..||:|.|:...
Human   628 --------LSFRHWPTLFSYYNITLAKRYISLLPVIPV---------TLRLNPREALEGRHPQDG 675

  Fly   781 -------------QWKALAAALDPLDFNDD-DVRRESPMVMAEGLPLVPKSPMEIFFAILLCCIS 831
                         .|:|.......:..:.| .:.:.:.:.:|.|:.||          :||.|:
Human   676 RSAWPPPGPIPAGHWEAGPKGPGGVQAHGDVTLYKVAALGLATGIVLV----------LLLLCL- 729

  Fly   832 IFVLCYTMVVFYRCICTRNYAEWRSS---WHESEAP---YKQTEQILEGVPTQIAGHKHRIECLV 890
                       ||.:|.|||.:....   ....|.|   |.......|.||..:.||...||||.
Human   730 -----------YRVLCPRNYGQLGGGPGRRRRGELPCDDYGYAPPETEIVPLVLRGHLMDIECLA 783

  Fly   891 SDGAYIISCCLKGQIRVWDARSGEQLTSISRS-------------DIQISQQR-TDG-------- 933
            |||..::||||.|.:.||||::|:.||.|.|.             :.|.|.:| :||
Human   784 SDGMLLVSCCLAGHVCVWDAQTGDCLTRIPRPGRQRRDSGVGSGLEAQESWERLSDGGKAGPEEP 848

  Fly   934 ----------------------------------------------------------------Q 934
                                                                            .
Human   849 GDSPPLRHRPRGPPPPSLFGDQPDLTCLIDTNFSAQPRSSQPTQPEPRHRAVCGRSRDSPGYDFS 913

  Fly   935 TLVRK-----------------------------------------LAVSP-----VWCLDYFDN 953
            .||::                                         ||.:|     :|.|:...|
Human   914 CLVQRVYQEEGLAAVCTPALRPPSPGPVLSQAPEDEGGSPEKGSPSLAWAPSAEGSIWSLELQGN 978

  Fly   954 LIAVGCANGRVELWESPAGLLKCAYQEDAKRNQGITHIHLNGDRVIVARLNGRLDFYRLETYYKG 1018
            ||.||.::||:|:|::..|:|.|:.:|   .:.|||.:.....|::.|||||.|||:.|||:...
Human   979 LIVVGRSSGRLEVWDAIEGVLCCSSEE---VSSGITALVFLDKRIVAARLNGSLDFFSLETHTAL 1040

  Fly  1019 KQIDWGFTSAYRRTHVRTGSTGSLGLMLQQQRCQQEASQKTTKEEMKITLEGVRLAHQQPITCMQ 1083
            ..:.      :|.|..|..|..|          ...:|..|....:..|   |..|||:|||.::
Human  1041 SPLQ------FRGTPGRGSSPAS----------PVYSSSDTVACHLTHT---VPCAHQKPITALK 1086

  Fly  1084 VVNDMVFTGSQDHTLKVYCLNKSDVEYTLHGHCGPVTCLFVDRWQPGTGGSGSQDGLLCVWDLFT 1148
            .....:.||||||||:|:.|..|...:||.||.|.:|.:::|  |.....||.|||.:|:||:.|
Human  1087 AAAGRLVTGSQDHTLRVFRLEDSCCLFTLQGHSGAITTVYID--QTMVLASGGQDGAICLWDVLT 1149

  Fly  1149 GACMYNIQAHDGAVSCLACAPSYVISLGTDERICVWERFQGNLLTTINIS-NAYSSLLMLTPSLL 1212
            |:.:.::.||.|.|:.|.|..|.|||.|.|:.|.:|:|..|....:|... ...:||.:::.:||
Human  1150 GSRVSHVFAHRGDVTSLTCTTSCVISSGLDDLISIWDRSTGIKFYSIQQDLGCGASLGVISDNLL 1214

  Fly  1213 VTSKMGSLIVWDVRTG--------------QPAREVKLDFANLQLCPKIMMLACDSVVCDYGNEI 1263
            ||...|.:..||:..|              ||||:             |::|...::||::|:|:
Human  1215 VTGGQGCVSFWDLNYGDLLQTVYLGKNSEAQPARQ-------------ILVLDNAAIVCNFGSEL 1266

  Fly  1264 RVVRFPIVADK 1274
            .:|..|.|.:|
Human  1267 SLVYVPSVLEK 1277

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
SCAP	NP_788277.1	Sterol-sensing	390..542	CDD:289145	81/151 (54%)
		WD40	<878..1227	CDD:225201	133/481 (28%)
		WD40	878..1185	CDD:295369	120/438 (27%)
		WD40 repeat	887..942	CDD:293791	28/181 (15%)
		WD40 repeat	945..983	CDD:293791	15/37 (41%)
		WD40 repeat	989..1070	CDD:293791	21/80 (26%)
		WD40 repeat	1079..1114	CDD:293791	15/34 (44%)
		WD40 repeat	1120..1157	CDD:293791	13/36 (36%)
		WD40 repeat	1162..1196	CDD:293791	13/33 (39%)
		WD40 repeat	1201..1233	CDD:293791	13/45 (29%)
SCAP	NP_036367.2	Loop-1. /evidence=ECO:0000250\|UniProtKB:P97260	46..284		77/241 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	60..80		1/19 (5%)
		Sterol-sensing	308..457	CDD:289145	76/148 (51%)
		ER export signal. /evidence=ECO:0000250\|UniProtKB:P97260	447..452		3/4 (75%)
		Loop-7. /evidence=ECO:0000250\|UniProtKB:P97260	535..710		54/216 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	579..615		9/40 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	668..696		9/27 (33%)
		Interaction with SREBF2. /evidence=ECO:0000250\|UniProtKB:P97260	731..1279		170/667 (25%)
		WD40	<771..813	CDD:295369	9/44 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	811..904		41/227 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	931..962		12/30 (40%)
		WD40	939..>1235	CDD:225201	100/303 (33%)
		WD40 repeat	959..1005	CDD:293791	11/45 (24%)
		WD40 repeat	1011..1047	CDD:293791	12/35 (34%)
		WD40	1078..>1235	CDD:295369	52/164 (32%)
		WD40 repeat	1082..1116	CDD:293791	14/33 (42%)
		WD40 repeat	1123..1157	CDD:293791	11/34 (32%)
		WD40 repeat	1163..1197	CDD:293791	12/47 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165142919
Domainoid	1	1.000	180	1.000	Domainoid score	I3520
eggNOG	1	0.900	-	-		E1_KOG1933
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8160
Inparanoid	1	1.050	593	1.000	Inparanoid score	I980
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48668
OrthoDB	1	1.010	-	-		D242783at33208
OrthoFinder	1	1.000	-	-		FOG0006370
OrthoInspector	1	1.000	-	-		oto89130
orthoMCL	1	0.900	-	-		OOG6_107255
Panther	1	1.100	-	-	LDO	PTHR46378
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3741
SonicParanoid	1	1.000	-	-		X6092
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.800