DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mle and Dhx36

DIOPT Version :9

Sequence 1:	NP_476641.1	Gene:	mle / 35523	FlyBaseID:	FBgn0002774	Length:	1293	Species:	Drosophila melanogaster
Sequence 2:	NP_082412.2	Gene:	Dhx36 / 72162	MGIID:	1919412	Length:	1001	Species:	Mus musculus

Alignment Length:	1082	Identity:	361/1082 - (33%)
Similarity:	556/1082 - (51%)	Gaps:	174/1082 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    88 GGPRTGLEGAGMAGGSGQQKRVFDGQSGPQDLGEAYRPLNHDGGDGGNRYSVIDRIQEQRDMNEA 152
            ||||.  .|.|.:||.|...|...|..|.:. |....|.:..|.:.|..|:     ::|...|:.
Mouse    13 GGPRG--SGQGSSGGGGGGSRGSGGGGGGRG-GRGRHPAHLKGREIGLWYA-----KKQTQKNKE 69

  Fly   153 EAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDDYKYTPVGPEHARSFLAELSIYVPALNRTVTA 217
            ......|.:|    ::..:|. .|.:..|:::         .:..:...|::|.:.|..:...|
Mouse    70 AERQERAVVH----MDERREE-QIVQLLNSVQ---------AKTDKDSEAQISWFAPEDHGYGT- 119

  Fly   218 RESGSNKKSASKSCALSLVRQLFHLNVIEPFSGTLKKKKDEQLKPYPVKLSPNLINKIDEVIKGL 282
             |..|.||..|                        :||.|.|.|        .|:|:..:..:
Mouse   120 -EVSSEKKINS------------------------EKKLDNQEK--------KLLNQEKKTFR-- 149

  Fly   283 DLPVVNPRNIKIELDGPPIPLIVNLSRIDSSQQDGEKRQESSVIPWAPPQANWNTWHACNIDEGE 347
                                 |.:.|.||         ::|..:                :.|.|
Mouse   150 ---------------------ITDKSYID---------RDSEYL----------------LQENE 168

  Fly   348 LATTSIDDLSMDYERSLRD--RRQNDNEYRQFLEFREKLPIAAMRSEILTAINDNPVVIIRGNTG 410
                  .:||:| :..|.|  |::.|..|.:...||:|||...|:.|::..||::.|.:|.|.||
Mouse   169 ------PNLSLD-QHLLEDLQRKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETG 226

  Fly   411 CGKTTQIAQYILDDYICSGQGGYANIYVTQPRRISAISVAERVARERCEQL--GDTVGYSVRFES 473
            ||||||:.|:|||:||..|:|....|..||||||||||||||||.||.|..  |::.||.:|.:|
Mouse   227 CGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRLQS 291

  Fly   474 VFPRPYGAILFCTVGVLLRKLEAG--LRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDLHVI 536
            ..||..|:||:||.|::|:.|::.  |..||||::||||||::.||.|:.:::|::....||.||
Mouse   292 RLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVI 356

  Fly   537 LMSATIDTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESRR------------K 589
            |||||::..|||:|||.||::.:||..|||.::.|||||:...:||..:..|            .
Mouse   357 LMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQGHVN 421

  Fly   590 RKEVEDEEQLLSEDKDEAEINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPG 654
            |:|.|::|.:..|....    |.|....:||..|.:.:.|:.:..|...|:.||:.:|..:...|
Mouse   422 RQEKEEKEAIYKERWPA----YIKELRTRYSASTVDVLQMMDDDKVDLNLIAALIRYIVLEEEDG 482

  Fly   655 AILVFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTKIILSTNI 719
            |||||||||:.|..|...|.:..:| .:.::.|:|.||.:|...|.:||:..|.||.||:::|||
Mouse   483 AILVFLPGWDNISTLHDLLMSQVMF-KSDKFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNI 546

  Fly   720 AETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRKGRAGRVRPGFCFTLCSRA 784
            |||||||||:|:|||..|.:...|.:.||:::.:..|.||.|.:|||||||||:||.|:.|.:..
Mouse   547 AETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGL 611

  Fly   785 RFQALEDNLTPEMFRTPLHEMALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDAN 849
            |...|:|...||:.||||.|:.|.||:||||.|.:|||:.::||..:||:.:...|.|:..||..
Mouse   612 RASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVVLSIKHLMELSALDKQ 676

  Fly   850 DELTPLGRLLARLPIEPRLGKMMVLGAVFGCAD-LMAIMASYSSTFSEVFSLDIGQRRLAN-HQK 912
            :||||||..|||||:||.:|||::.||:|.|.| ::.|.||.|  |.:.|.:.:|:.::|: .:|
Mouse   677 EELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLS--FKDPFVIPLGKEKIADARRK 739

  Fly   913 ALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWK-GLQMSTMNVIWDAKQQLLDLLQQAGF 976
            .|:....|||:.::.|.:.|...|:||...|..:| |: .|..:|:.::.:.|.|..:.|..|||
Mouse   740 ELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYC-WEYFLSSNTLQMLHNMKGQFAEHLLGAGF 803

  Fly   977 PEECMISHEVDERIDGDDPVLDVS-------LALLCLGLYPNICVHK----EKRKVLTTESKA-- 1028
                     |..| ...||..:::       .|::|.||||.:...:    :|||::...:|:
Mouse   804 ---------VSSR-SPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDG 858

  Fly  1029 -ALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQLSMVSPLQVILFGSRKIDLAA-----N 1087
             ..:|..|||...  ..|.|.:.::..|:||.::.....:.|||..::.||.   |::.     .
Mouse   859 LVSIHPKSVNVEQ--TDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGG---DISIQKDKDQ 918

  Fly  1088 NIVRVDNWLNFDIEPELAAKIGALKPALEDLITVACDNPSDI 1129
            .|:.||.|:.|.....:|..:..|:..|:.|:....::|..:
Mouse   919 EIIAVDEWIVFQSPERIAHLVKGLRKELDSLLQEKIESPHPV 960

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mle	NP_476641.1	DSRM	4..68	CDD:214634
		DSRM	170..240	CDD:214634	12/69 (17%)
		DEXDc	393..569	CDD:214692	93/179 (52%)
		DEXDc	402..542	CDD:238005	77/143 (54%)
		ATP-synt_B	<584..644	CDD:304375	15/71 (21%)
		Helicase_C	643..772	CDD:278689	60/128 (47%)
		HA2	842..926	CDD:214852	37/85 (44%)
		OB_NTP_bind	974..1078	CDD:285018	29/117 (25%)
Dhx36	NP_082412.2	Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..193		55/290 (19%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000250\|UniProtKB:D4A2Z8	1..97		24/105 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..54		15/43 (35%)
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..44		13/33 (39%)
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	46..98		11/70 (16%)
		DSM (DHX36-specific motif). /evidence=ECO:0000250\|UniProtKB:Q05B79, ECO:0000250\|UniProtKB:Q9H2U1	46..68		5/26 (19%)
		RecA-like domain 1. /evidence=ECO:0000250\|UniProtKB:Q05B79	99..379		126/368 (34%)
		HrpA	188..894	CDD:224557	291/725 (40%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	258..310		29/51 (57%)
		DEAH box. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00541	327..330		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250\|UniProtKB:Q05B79	380..621		96/245 (39%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	491..550		23/59 (39%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	510..521		4/10 (40%)
		WH domain. /evidence=ECO:0000250\|UniProtKB:Q05B79	622..691		36/68 (53%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	631..690		29/58 (50%)
		OB-fold-like subdomains. /evidence=ECO:0000250\|UniProtKB:Q05B79	834..898		14/65 (22%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	842..853		3/10 (30%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	863..893		9/31 (29%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG1643
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D222681at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.820