Protein Alignment mle and cdc28

DIOPT Version :10

• Sequence 1: NP_476641.1
• Gene: mle / 35523
• FlyBaseID: FBgn0002774
• Length: 1293
• Species: Drosophila melanogaster
• Sequence 2: NP_595686.2
• Gene: cdc28 / 2540707
• PomBaseID: SPBC19C2.01
• Length: 1055
• Species: Schizosaccharomyces pombe
• Alignment Length: 1162
• Identity: 282/1162 - (24%)
• Similarity: 457/1162 - (39%)
• Gaps: 282/1162 - (24%)

  Fly     3 IKSFLYQFCAK-----SQIEPKFDIRQTGPKNRQRFL--------CEVRVEPNTYIGVGNSTNKK 54
            :|.|.:...|:     |:.:..::.|:   |..|..|        .::.:||.::...|....||
pombe    55 VKEFAHTLYARIPREGSRPKENYNARK---KKEQGILQMERLNSSYDLLIEPQSHETPGKPLKKK 116

  Fly    55 DAEKNACRDFVNYLVRVGKLNTNDVPADAGASGGGPRTGLEGAGMAGGSGQQKRVFDGQSGPQDL 119
            ...|...|:...      :....|                     ...|.:.:.|.|
pombe   117 SRSKTPKREIAR------RQRDED---------------------EWESDEYEEVVD-------- 146

  Fly   120 GEAYRPL----------NHDGGDGGNRYSVIDRIQEQR--DMNEAEAFDVNAAIHGNWTIENAKE 172
            |.|..|:          |||       |......:.:|  |:.|.|.|            |....
pombe   147 GSASHPIEEDSVSTDFQNHD-------YEKSSDPETERLNDLREREEF------------EERLR 192

  Fly   173 RLNIYKQTNNIRDDY--KYTPV---------GPEHARSFLAELSIYVPALNRTVTARESGSNKKS 226
            |.::...||...:||  |::..         .||..|...:||                  .|||
pombe   193 RKDLEAATNEFVEDYSSKFSSEELALRKLADDPESWRKLASEL------------------RKKS 239

  Fly   227 ASKSCALSLVRQLFHLNVIEPFSGTLKKKKDEQLKPYPVKLSPNLINKIDEVIKGLDLPVVNPRN 291
            ..:.......:||..|.         ::.:||:......||:.   .:|.|:.|..:|..:....
pombe   240 RQQYLKPRAQQQLEILR---------REIRDEEQLFAGEKLTQ---AEIRELEKKKELLRIAEER 292

  Fly   292 IKIELDGP----PIPLIVNLSRIDSSQ---------QDGEKRQESSVIPWAPPQANW-------- 335
            .::|....    |........::|..:         :|..:.:::.|...|..|..|
pombe   293 QRLEKQATEYQMPEDYFTEQGKLDRKRKEEVLYQRYKDSNEGEQNEVTMGAAEQQRWEAQQINKA 357

  Fly   336 -----NTWHACNIDEGEL-------ATTSID---DLSMDYERSLRDRRQNDNEYRQFLE-FREKL 384
                 |.|    :..||.       .:..||   |..:..|..:...:..|.:..:.|| .|:.|
pombe   358 LLFDQNEW----LPPGEKQFDFVFDESQQIDFLLDTKLSAENPVDTDKMTDVKVEKSLESSRKSL 418

  Fly   385 PIAAMRSEILTAINDNPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGYA----NIYVTQPRRIS 445
            |:...:.::|.|||:..|::|...||.|||||:.|::       .:.||.    .|..|||||::
pombe   419 PVYQYKDDLLKAINEYQVLLIVAETGSGKTTQLPQFL-------HEAGYTKGNKKICCTQPRRVA 476

  Fly   446 AISVAERVARERCEQLGDTVGYSVRFESVFPRPYGAILFCTVGVLLRKL--EAGLRGVSHIIVDE 508
            |:|||.|||:|...:||..||||:|||:..... ..|.:.|.|:|||:.  |..|...|.||:||
pombe   477 AMSVAARVAKEMDVRLGQEVGYSIRFENATSEK-TVIKYLTDGMLLREFLTEPDLASYSVIIIDE 540

  Fly   509 IHERDVNSDFLLVILRDMVDTYPDLHVILMSATIDTTKFSKYFGICPVLEVPGRAFPVQQFFLED 573
            .|||.:::|.|..:::|:....|||.|::.|||||..|||.||...||..||||.:||..::
pombe   541 AHERTLHTDILFGLVKDIARFRPDLKVLISSATIDAEKFSAYFDEAPVFYVPGRRYPVDIYY--- 602

  Fly   574 IIQMTDFVPSAESRRKRKEVEDEEQLLSEDKDEAEINYNKVCEDKYSQKTRNAMAMLSESDVSFE 638
                                          ..:.|.||                         .:
pombe   603 ------------------------------TPQPEANY-------------------------IQ 612

  Fly   639 LLEALLMHIKSKNIPGAILVFLPGWNLIFALMKFLQN-TNIFGDTSQYQIL-PCHSQIPRDEQRK 701
            .....::.|.:....|.|||||.|.:.|..:.:.:|. ..|.|......|| |.::.:|.:.|.|
pombe   613 AAITTILQIHTTQPAGDILVFLTGQDEIELMSENMQELCRILGKRIPEIILCPIYANLPSELQAK 677

  Fly   702 VFEPVPEGVTKIILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRK 766
            :|:|.|.|..|::|:|||||||||||.:.||||....:..::.....:.|..:|..|:.:.:||.
pombe   678 IFDPTPPGARKVVLATNIAETSITIDGVNFVIDSGFVKQNMYNPRTGMESLVSVPCSRASADQRA 742

  Fly   767 GRAGRVRPGFCFTLCSRARFQALEDNLT-PEMFRTPLHEMALTIKLLRLGSIHHFLSKALEPPPV 830
            ||||||.||.||.|.:|..:....|.:| ||:.||.|..:.|.:|.|.:.::..|  ..::.||.
pombe   743 GRAGRVGPGKCFRLYTRRTYNNELDMVTSPEIQRTNLTNIVLLLKSLGINNLLDF--DFMDAPPP 805

  Fly   831 DAVIEAEVLLREMRCLDANDELTPLGRLLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFS 895
            :.::.:..||..:..|:...|||.|||.:|..|.:|.|.|.::..:.:||.:.:..:.|.....|
pombe   806 ETLMRSLELLYALGALNNRGELTKLGRQMAEFPTDPMLSKSLIASSKYGCVEEVLSIVSMLGEAS 870

  Fly   896 EVFSLDIGQRRLANHQKALSGTKCSDHVAMIVA---------SQMWRREKQRGEHMEARFCDWKG 951
            .:|.....:...|:..:|.......||:.::..         |..|.||         .|..:|.
pombe   871 SLFYRPKDKIMEADKARANFTQPGGDHLTLLHIWNEWVDTDFSYNWARE---------NFLQYKS 926

  Fly   952 LQMSTMNVIWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNIC-VH 1015
            |..:.     |.:.||.:|.::.          |: |.:......||.....:..|.:.|.. :.
pombe   927 LCRAR-----DVRDQLANLCERV----------EI-ELVTNSSESLDPIKKAITAGYFSNAARLD 975

  Fly  1016 KEKRKVLTTES-KAALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQLSMVSP 1071
            :......|.:| :...:|.:    |::|...| ...::.|.:.|....|:|::.:.|
pombe   976 RSGDSYRTVKSNQTVYIHPS----SSVAEKKP-KVIIYFELVLTTKEYCRQITEIQP 1027

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mle	NP_476641.1	DSRM_DHX9_rpt1	2..70	CDD:380683	15/79 (19%)
		DSRM_DHX9_rpt2	167..241	CDD:380684	17/84 (20%)
		DEXHc_DHX9	326..559	CDD:350730	91/262 (35%)
		HrpA	378..>889	CDD:441249	173/520 (33%)
		OB_NTP_bind	1002..1079	CDD:400182	13/72 (18%)
cdc28	NP_595686.2	PTZ00121	<68..>357	CDD:173412	62/375 (17%)
		DUF4670	<213..322	CDD:464815	22/138 (16%)
		HrpA	417..>1039	CDD:441249	205/709 (29%)