Protein Alignment mle and DHX36

DIOPT Version :10

• Sequence 1: NP_476641.1
• Gene: mle / 35523
• FlyBaseID: FBgn0002774
• Length: 1293
• Species: Drosophila melanogaster
• Sequence 2: NP_065916.2
• Gene: DHX36 / 170506
• HGNCID: 14410
• Length: 1008
• Species: Homo sapiens
• Alignment Length: 1083
• Identity: 354/1083 - (32%)
• Similarity: 559/1083 - (51%)
• Gaps: 166/1083 - (15%)

  Fly    81 ADAGASGGGPRTGLEGAGMAGGSGQQKRVFDGQSGPQDLGEAYRPLNHDGGDGGNRYSVIDRIQE 145
            :..|..||||..|..|...:||.|       |..|....|....|.:..|.:.|..|:     ::
Human    17 SSGGGYGGGPAGGHGGNRGSGGGG-------GGGGGGRGGRGRHPGHLKGREIGMWYA-----KK 69

  Fly   146 QRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDDYKYTPVGPEHARSFLAELSIYVPA 210
            |...|:.......|.:|    ::..:|. .|.:..|:::         .::.:...|::|.:.|
Human    70 QGQKNKEAERQERAVVH----MDERREE-QIVQLLNSVQ---------AKNDKESEAQISWFAP- 119

  Fly   211 LNRTVTARESGSNKKSASKSCALSLVRQLFHLNVIEPFSGTLKKKKDEQLKPYPVKLSPNLINKI 275
                   .:.|...:.::|:...|                  :.|.|.|.|        .|||:.
Human   120 -------EDHGYGTEVSTKNTPCS------------------ENKLDIQEK--------KLINQE 151

  Fly   276 DEVIKGLDLPVVNPRNIKIELDGPPIPLIVNLSRIDSSQQDGEKRQESSVIPWAPPQANWNTWHA 340
            .::.:                       |.|.|.||   :|.|...:.
Human   152 KKMFR-----------------------IRNRSYID---RDSEYLLQE----------------- 173

  Fly   341 CNIDEGELATTSIDDLSMDYERSLRDRRQNDNEYRQFLEFREKLPIAAMRSEILTAINDNPVVII 405
             |..:|.|....::||         .:::||..|.:...||||||...|:.|::..|:::.|.:|
Human   174 -NEPDGTLDQKLLEDL---------QKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVI 228

  Fly   406 RGNTGCGKTTQIAQYILDDYICSGQGGYANIYVTQPRRISAISVAERVARERCEQL--GDTVGYS 468
            .|.||||||||:.|:|||:||..|:|....|..||||||||||||||||.||.|..  |::.||.
Human   229 SGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQ 293

  Fly   469 VRFESVFPRPYGAILFCTVGVLLRKLEAG--LRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYP 531
            :|.:|..||..|:||:||.|::|:.|::.  |..||||::||||||::.||.|:.:::|:::...
Human   294 IRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRS 358

  Fly   532 DLHVILMSATIDTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESRR-------- 588
            ||.|||||||::..|||:|||.||::.:||..|||.::.|||:|:...:||..:..|
Human   359 DLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRSQFKRGFM 423

  Fly   589 ----KRKEVEDEEQLLSEDKDEAEINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKS 649
                .|:|.|::|.:..|...:    |.:....:||..|.:.:.|:.:..|...|:.||:.:|..
Human   424 QGHVNRQEKEEKEAIYKERWPD----YVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVL 484

  Fly   650 KNIPGAILVFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTKII 714
            :...||||||||||:.|..|...|.:..:| .:.::.|:|.||.:|...|.:||:..|.||.||:
Human   485 EEEDGAILVFLPGWDNISTLHDLLMSQVMF-KSDKFLIIPLHSLMPTVNQTQVFKRTPPGVRKIV 548

  Fly   715 LSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRKGRAGRVRPGFCFT 779
            ::||||||||||||:|:|||..|.:...|.:.||:::.:..|.||.|.:|||||||||:||.|:.
Human   549 IATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYH 613

  Fly   780 LCSRARFQALEDNLTPEMFRTPLHEMALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMR 844
            |.:..|...|:|...||:.||||.|:.|.||:||||.|.:|||:.::||..:||:.:...|.|:.
Human   614 LYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELN 678

  Fly   845 CLDANDELTPLGRLLARLPIEPRLGKMMVLGAVFGCAD-LMAIMASYSSTFSEVFSLDIGQRRLA 908
            .||..:||||||..|||||:||.:|||::.||:|.|.| ::.|.||.|  |.:.|.:.:|:.::|
Human   679 ALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLS--FKDPFVIPLGKEKIA 741

  Fly   909 N-HQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWK-GLQMSTMNVIWDAKQQLLDLL 971
            : .:|.|:....|||:.::.|.:.|...::||...|..:| |: .|..:|:.::.:.|.|..:.|
Human   742 DARRKELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYC-WEYFLSSNTLQMLHNMKGQFAEHL 805

  Fly   972 QQAGF-----PEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNICVHK----EKRKVLTTESK 1027
            ..|||     |:      :.:..|:.|:.  .:..|::|.||||.:...:    :|||::...:|
Human   806 LGAGFVSSRNPK------DPESNINSDNE--KIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTK 862

  Fly  1028 A---ALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQLSMVSPLQVILFGSRKIDLAANN- 1088
            .   ..:|..|||...  ..|.|.:.::..|:||.::.....:.|||..::.||. .|.:..:|
Human   863 TDGLVAVHPKSVNVEQ--TDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGG-DISIQKDND 924

  Fly  1089 --IVRVDNWLNFDIEPELAAKIGALKPALEDLITVACDNPSDI 1129
              .:.||.|:.|.....:|..:..|:..|:.|:....::|..:
Human   925 QETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPV 967

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mle	NP_476641.1	DSRM_DHX9_rpt1	2..70	CDD:380683
		DSRM_DHX9_rpt2	167..241	CDD:380684	9/73 (12%)
		DEXHc_DHX9	326..559	CDD:350730	103/236 (44%)
		HrpA	378..>889	CDD:441249	237/527 (45%)
		OB_NTP_bind	1002..1079	CDD:400182	23/83 (28%)
DHX36	NP_065916.2	Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000269|PubMed:18279852	1..200		52/295 (18%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000250|UniProtKB:D4A2Z8	1..104		24/112 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..58		14/47 (30%)
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000269|PubMed:18854321	1..51		13/40 (33%)
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000269|PubMed:21149580, ECO:0000269|PubMed:21846770, ECO:0000269|PubMed:24151078	53..105		11/70 (16%)
		DSM (DHX36-specific motif). /evidence=ECO:0000269|PubMed:20472641, ECO:0000269|PubMed:22238380, ECO:0000269|PubMed:24369427, ECO:0000269|PubMed:26195789, ECO:0000269|PubMed:26649896	53..75		5/26 (19%)
		RecA-like domain 1. /evidence=ECO:0000250|UniProtKB:Q05B79	106..386		123/366 (34%)
		DEXHc_DHX36	207..386	CDD:350739	91/178 (51%)
		HrpA	215..>776	CDD:441249	246/567 (43%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	265..317		29/51 (57%)
		DEAH box. /evidence=ECO:0000255|PROSITE-ProRule:PRU00541	334..337		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250|UniProtKB:Q05B79	387..628		94/245 (38%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	498..557		23/59 (39%)
		Nuclear localization signal. /evidence=ECO:0000303|PubMed:14731398	517..528		4/10 (40%)
		WH domain. /evidence=ECO:0000250|UniProtKB:Q05B79	629..698		36/68 (53%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	638..697		29/58 (50%)
		OB_NTP_bind	831..917	CDD:400182	24/88 (27%)
		OB-fold-like subdomains. /evidence=ECO:0000250|UniProtKB:Q05B79	841..905		14/65 (22%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	849..860		3/10 (30%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	870..900		9/31 (29%)