DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment BubR1 and Bub1

DIOPT Version :9

Sequence 1:	NP_477382.1	Gene:	BubR1 / 35522	FlyBaseID:	FBgn0263855	Length:	1460	Species:	Drosophila melanogaster
Sequence 2:	NP_001106650.1	Gene:	Bub1 / 12235	MGIID:	1100510	Length:	1059	Species:	Mus musculus

Alignment Length:	1457	Identity:	315/1457 - (21%)
Similarity:	511/1457 - (35%)	Gaps:	484/1457 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly    41 KQMEEEVRTYKGADPLGAWYTFICWIEQSYPAGGSGSGLQTVLHQCLTKFEDDERYRQDKRLIKL 105
            :..|..:::|.|.||||.|.:||.|:|:::|  .:...|.|:|...:.:|...:.|..|.|.|..
Mouse     9 RMFEAHMQSYTGNDPLGEWESFIKWVEENFP--DNKEYLMTLLEHLMKEFLHKKNYHNDSRFINY 71

  Fly   106 FIKFMEKQKDKIEFYQQMYNNGIGTMLADFYIAWAYSYDLSGNMRKADEIFRLGLECRAEPLEDL 170
            .:||.|...|:.:|::.:||.||||..:..|::||...:..|.::.|..||:.|:...|||.|.|
Mouse    72 CLKFAEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKELL 136

  Fly   171 KEAHHHFGYTVGQRMLYSTGEEANAVNQELNERRLALQSLHGRRQQISNSITVGSIRTGAAVKSG 235
            ::.:..|              :|......|..:....:.||.  .||.|.:.:
Mouse   137 QQQYRLF--------------QARLTGIHLPAQATTSEPLHS--AQILNQVMM------------ 173

  Fly   236 LPGVVQVEPSTTTSRRNVGRNVEVFNDENSEGNIPISNSETEVKSSLRSIIDSACSQENIKEPVA 300
                       |.|           :.|.:...:|.|..     |....:..|.|.:
Mouse   174 -----------TNS-----------SPEKNSACVPRSQG-----SECSGVASSTCDE-------- 203

  Fly   301 WNKANAKRHKHGKIFGSNASPDLGFDIHVDEQAMPPITNYERRLEQPFRFPTNFVAKNRPQEPWV 365
              |:|.:..:...|..|..|        |.....|                       :|:...|
Mouse   204 --KSNIREQRVIMISKSECS--------VSSSVAP-----------------------KPEAQQV 235

  Fly   366 TPVTIEDEPNASGLPCYNKCLLYPRPNIEFSPEEYRAYSFLKHRNPQHPFIHRNDDWWGTGRVIR 430
                         :.|..|.:   |.:.|||.||.||..:.:.:        :::.|....|
Mouse   236 -------------MYCKEKLI---RGDSEFSFEELRAQKYNQRK--------KHEQWVSEDR--- 273

  Fly   431 GIRCYPNFARYSKPQDLDELDKFWKPPLVPGLQVVFDKIYNEVEQKEYQPEELLAAKWMEKRNVT 495
                     .|.|.::.:..::          |::..|: :|:.:|.:|..||
Mouse   274 ---------NYMKRKEANAFEE----------QLLKQKM-DELHKKLHQVVEL------------ 306

  Fly   496 VHGDFDMEET------VCLPGNKMPRRKSFFPSSFRKSIMPRRVSSVQEEDEKKEDEATLIVKDI 554
            .|.|....|.      ||:..|...:::...||   .|.:..:.|....|..::|....|:|..:
Mouse   307 SHKDLPASENRPDVSLVCVGQNTCSQQELRGPS---LSSISHQTSESSGEKPQEEPSVPLMVNAV 368

  Fly   555 PSQVAPKIPESPCDQIDEPKIKIFEDSGSPRDNFAVPAVPVP-KIEIYEDSE---------EPQP 609
            .|.:                  :|     |..|  :||:||| ..:...||.         .||.
Mouse   369 NSTL------------------LF-----PAAN--LPALPVPVSGQSLTDSRCVNQSVHEFMPQC 408

  Fly   610 TPKTRPFYDGDETCSTQMFNMFIKSQAVSTPKGTQKQAPSRQFGTVLKELPLPEEAAHSVESPVE 674
            .|:|:      |.|.|.      |..:::....|...:.....||..|..|.|  ..|:.|:
Mouse   409 GPETK------EVCETN------KVASINDFHTTPNTSLGMVQGTPCKVQPSP--TVHTKEA--- 456

  Fly   675 TRSPTLRKQLSTILETSEHGTQSLATSGTTTKSTITSTSSPGSNAVSKLGSKIEENTPGQMRLQR 739
                     |..|::..:
Mouse   457 ---------LGFIMDMFQ----------------------------------------------- 465

  Fly   740 VISAGVSAVLSEPEKPGGDNFLRRELWEPNAPSVPMLKSLRFQEDKTETIPRPLACFQEDKTETL 804
                                          ||::|.:     .:||.|.   |.....||..|..
Mouse   466 ------------------------------APTLPDI-----SDDKDEW---PSLDQNEDAFEAQ 492

  Fly   805 PQMPIALPENSMDHGSLGAENCFS-APQLPTLEDESDLCGLFGKTPPKTNIFGSPKRNFDPTSQL 868
            .|      :|::..|..|.:...: :...|..||.:           |.| :|.|:....|   |
Mouse   493 FQ------KNAVSSGDWGVKKIMTLSSAFPIFEDGN-----------KEN-YGLPQPKNKP---L 536

  Fly   869 FKSTQGDRRSFAVTGNKLEAAPSISNLQDSFMCDL----SFVPETQPEAVAKSGAIQKFDIFLDD 929
            ...|.|:|.....:....|...:...:.||.:|.:    :..|  .|:::.            |.
Mouse   537 GARTFGERSLSKYSSRSNEMPHTDEFMDDSTVCGIRCNKTLAP--SPKSIG------------DF 587

  Fly   930 TQPKLTKSKPVALMVSSFDLDCT-LPETQQVAIKEPGTQQENIGHSHMIASFMKDCTEIGSCPSQ 993
            |......|.|    ...|..|.. :||.::..:   .||     ::||         .:.||
Mouse   588 TSAAQLSSTP----FHKFPADLVQIPEDKENVV---ATQ-----YTHM---------ALDSC--- 628

  Fly   994 MPASVVKTNISSTSNKIKFSNDSMSESSRKAPVNENVGIS----KQP--DEFFELNAATEMFATN 1052
                  |.||.           .:|:..:..|:.|.:..|    .||  ...|...|...:.|..
Mouse   629 ------KENIV-----------DLSKGRKLGPIQEKISASLPCPSQPATGGLFTQEAVFGLEAFK 676

  Fly  1053 ISMIKNSTLLIPNAKVAEASELSIYYKTTPLTPKQSHRSWSQSDLETPPRENFVHPTSNGDQTVL 1117
            .:.|.::|:                                 .||.         ..:.|.|...
Mouse   677 CTGIDHATV---------------------------------EDLS---------DANAGLQVEC 699

  Fly  1118 NETLADAN------KNPFNVELISSLLESI-----DFSMYIE---KLPHCQLVGHVKRLHPNTHL 1168
            .:||.:.|      :||::.|||..||..:     .:|...|   |||         .:...|..
Mouse   700 VQTLGNVNAPSFTVENPWDDELILKLLSGLSKPVTSYSNTFEWQSKLP---------AIKTKTEY 755

  Fly  1169 EVHNEKFEVSKMIGKGAYGSVYVGKH-----LKSGKKVALKQERPTNYWEFYICLEIHSRLTSE- 1227
            ::.:....|:.::|:||:..|:...|     .||.:|..||.:||.|.|||||.:::..||..|
Mouse   756 QLGSLLVYVNHLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEV 820

  Fly  1228 --QMIPSY-AHIDYALVGNNSSVYISEFSDYGSLIGVCNKVKSVTNRNMDEYVVMHLSCQMLDIV 1289
              ..|..| ||:     ..|.|:.:.|...||:|:.|.|..|:.:.:.|.:.:|:..:.:||.:|
Mouse   821 HHMFIKFYSAHL-----FKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMV 880

  Fly  1290 DHLHAMGIIHADIKPDNFLLMKPICADPNE---VSLQLIDFGVSIDMKLFPDNQTFNYVHHDDLF 1351
            :.:|:..|||.|||||||:|........:|   ..|.|||.|.||||||||....|........|
Mouse   881 EQVHSCEIIHGDIKPDNFILGHRFLEQADEDLATGLALIDLGQSIDMKLFPKGTVFTGKCETSGF 945

  Fly  1352 KCIEMRTGRPWTYQLDLYGLVSVMHVLLFGRYMEVVQRSPSTIWMPKTNVPRYFQRTMWENIFRT 1416
            :|.||.:.:||.||:|.:|:.:.::.:|||.||:|  ::...:|.|:....|.....|||..|..
Mouse   946 QCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKV--KNEGGVWKPEGLFRRLPHLDMWEEFFHI 1008

  Fly  1417 LLNIRDCRTMPNLQQLRTQLKCALAEK 1443
            :|||.||..:|:|..||..:|..|.::
Mouse  1009 MLNIPDCHNLPSLDFLRQNMKKLLEQQ 1035

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
BubR1	NP_477382.1	Mad3_BUB1_I	38..159	CDD:285506	39/117 (33%)
BubR1	NP_477382.1	STKc_Bub1_BubR1	1174..1458	CDD:270883	101/282 (36%)
Bub1	NP_001106650.1	Necessary for kinetochore localization. /evidence=ECO:0000250	1..146		46/152 (30%)
		Mad3_BUB1_I	6..125	CDD:285506	39/117 (33%)
		Nuclear localization signal. /evidence=ECO:0000255	58..65		1/6 (17%)
		Necessary for interaction with KNL1. /evidence=ECO:0000250	99..132		9/32 (28%)
		Necessary for interaction with BUB3. /evidence=ECO:0000250	230..256		10/41 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	335..360		6/27 (22%)
		Essential for loading of BUBR1, MAD1L1 and MAD2L1 to kinetochores. /evidence=ECO:0000250	447..465		6/31 (19%)
		KEN box 1. /evidence=ECO:0000250	523..525		1/1 (100%)
		KEN box 2. /evidence=ECO:0000250	612..614		0/1 (0%)
		PKc_like	761..1047	CDD:304357	101/282 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167831999
Domainoid	1	1.000	143	1.000	Domainoid score	I4633
eggNOG	1	0.900	-	-		E1_KOG1166
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	277	1.000	Inparanoid score	I2921
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1411806at2759
OrthoFinder	1	1.000	-	-		FOG0004065
OrthoInspector	1	1.000	-	-		otm42662
orthoMCL	1	0.900	-	-		OOG6_103677
Panther	1	1.100	-	-	LDO	PTHR14030
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2052
SonicParanoid	1	1.000	-	-		X2819
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	14	13.830