Protein Alignment BubR1 and bub1

DIOPT Version :10

• Sequence 1: NP_477382.1
• Gene: BubR1 / 35522
• FlyBaseID: FBgn0263855
• Length: 1460
• Species: Drosophila melanogaster
• Sequence 2: XP_002665685.2
• Gene: bub1 / 100307076
• ZFINID: ZDB-GENE-081104-75
• Length: 1202
• Species: Danio rerio
• Alignment Length: 1529
• Identity: 343/1529 - (22%)
• Similarity: 555/1529 - (36%)
• Gaps: 463/1529 - (30%)

  Fly    44 EEEVRTYKGADPLGAWYTFICWIEQSYPAGGSGSGLQTVLHQCLTKFEDDERYRQDKRLIKLFIK 108
            |..||:|.|.|||..|..|:.::| |..:.....||..||.:.:..|..||||..|.|.:...|.
Zfish    11 ELSVRSYAGDDPLDPWDKFVHFLE-SRMSAEEKKGLSVVLERLVQSFLQDERYHNDVRYVTHCIT 74

  Fly   109 FMEKQKDKIEFYQQMYNNGIGTMLADFYIAWAYSYDLSGNMRKADEIFRLGLECRAEPLEDLKEA 173
            ......|.:|.|..:::.|:||..|..||.||...:..|.|.:|:.::...|..:|:|.:.::|.
Zfish    75 CANLYSDPMEVYSYLHSRGVGTQTAALYIDWAQQCEKKGQMVQAEMVYNRALMNKAQPQDTVQEQ 139

  Fly   174 HHHFGYTVGQRMLYST-GEEANAVNQELNERRLALQSLHGRRQQISNSITVGSIRTGAAVKSGLP 237
            :..|    .:|...|| ...::.|...|...|:..|:
Zfish   140 YRFF----QERTSASTPAAPSDHVRHPLQNSRMVNQN---------------------------- 172

  Fly   238 GVVQVEPSTTTSRRNVGRNVEVFNDENSEGNIPISNSETEVKSSLRSIIDSACSQENIKEPVAWN 302
                     |..|.||                                      |...|||.|
Zfish   173 ---------TRPRDNV--------------------------------------QPQCKEPEA-- 188

  Fly   303 KANAKRHKHGKIFGSNASPDLGFDIHVDEQAMPPITNYERRLEQPFRFPTNFVAKNRPQEP--WV 365
                            |.|            .|.:.:...|.|            |.|..|  :.
Zfish   189 ----------------ACP------------APRMVSVISRSE------------NNPVRPAGYT 213

  Fly   366 TPVTIEDEPNASGLPCYN--KCLLYPRPNIEFSPEEYRAYSFL---KHRNPQHPFIHRNDDWWGT 425
            .|.|:       .:.|..  :|     ...|.|.||.||..:.   :.:..|..........|..
Zfish   214 APETV-------SMYCVKELQC-----EGSELSFEELRARRYFDKCRQQEEQRRLAEEQQRLWEE 266

  Fly   426 GRVIRGIRCYPNFARYSKPQDLDELDK--FWKP-----PLVPGLQVVFDKIYNEVEQ--KEYQPE 481
            ...:|.::           |.||:|::  ..||     |:.|.:.......:.:.:.  ..|.|.
Zfish   267 EEEVRKLK-----------QLLDDLERNMSLKPAEQTDPVTPAVMEQQGLAFQQADPPVTSYSPA 320

  Fly   482 ELLAAKWMEKRNVTVHGDFDMEETVCLPGNKMPRRKSFFPSSFRKSIMPRRVSSVQEEDEKKEDE 546
            |......::.|                     |.|:|......|.|.:...|...::||...:.|
Zfish   321 EPFLTAALDSR---------------------PERESVLEQPDRPSEIQSSVPQSRQEDVCVQGE 364

  Fly   547 ATL-----------------IVKDIPSQVAPKIPESP-----------CDQIDEPKI---KIFED 580
            |..                 .|:..||:|.|    ||           .|....|.:   .:|..
Zfish   365 ADTSYGGFNHSHVTPNTSLGFVQATPSRVLP----SPTVNTREALGVIMDMFQAPTLLQESMFNS 425

  Fly   581 SGSPRDNF-----------AVPAVPVPKIEIYEDSEEPQPTPKTRPFYDGDETCSTQMFNMFIKS 634
            |..|.|:|           .|.|.......||:|.::                           |
Zfish   426 SVQPEDSFEKSCRISSAPSCVKAASATPFMIYQDRDQ---------------------------S 463

  Fly   635 QAVSTPKGTQKQAPSRQFGTVLKELPLPEEAAHSVESPVETRSPTLRKQLSTILETSEHGTQSLA 699
            |:..:.:...|.|.:|    .|.|:|:    :.:.|:||.|...|        .|::..|.::::
Zfish   464 QSAGSDQAGNKPAVTR----ALMEIPV----SKANETPVSTEFIT--------DESTMWGARNVS 512

  Fly   700 TSGTTTKSTITSTSSPGSNAVSKLGSKIEENTP-----GQMRLQRVISAGVSAVLSEPEKPGGDN 759
            .......   |...|..::.||   :.:..:.|     .|.:.:..:..||||....|
Zfish   513 VGPCPNH---TRDFSLSAHLVS---TPLHPHAPHSWDAEQSQEENAVVRGVSAAEETP------- 564

  Fly   760 FLRRELWEPNAPSVPMLKSLRFQEDKTETIPRPLACFQEDKTETLPQMPIALPENSMDHGSLGAE 824
            :||    :|...| |:::....:|       :|..|..:.: .|:....::|.:.|... ||...
Zfish   565 YLR----QPTKLS-PIMEQSPAEE-------KPAECSMQAQ-GTIVGEGVSLAQQSQGL-SLAQS 615

  Fly   825 NCFSAPQLPTL----------EDESDLCGLFGKTPPKTNIFGSPKRNFDPT-------SQLFKST 872
            :|.|...|..|          :|||    |...:.|..:|:.||::  :|:       ..|..:|
Zfish   616 SCSSRHPLAALSFPDHTALRAQDES----LRNSSRPAWSIYQSPEK--EPSLLDTAKDEDLLPTT 674

  Fly   873 QGDRRSFAVTGNK-LE----------AAPSISNLQ-DSFM------CDLSFVPETQPEAVAKSGA 919
            ...|.|   :|.| ||          |||.::..| ||..      .|:...|...|.|
Zfish   675 SFHRHS---SGMKPLETPDIPDMSPRAAPGLNWFQMDSSTHAAEPDLDVMMSPPPVPGA------ 730

  Fly   920 IQKFDIFLDDTQPKLT------KSKPVA-LMVSSFDLDCTLPETQQVAIKEPGTQQENIGHSHMI 977
               ::|:....:|..|      ..||:| ..:...::|..||| :....:|||.:...:.|..::
Zfish   731 ---WNIYQSPEKPPKTDFLWAMSPKPIAEHALHMEEVDVLLPE-RSFLQREPGVEALQVLHESLL 791

  Fly   978 ASFMKDCTEIGSCPSQMPASVVKTNISSTSNKIKFSNDSMSESSRKAPVNENVGISKQPDEFFEL 1042
            .|           |.:.||:..:.:.|..:       |....:|.:.||.:::.:...|.:
Zfish   792 MS-----------PKRTPAAAQEEHWSPRA-------DWSHTASPQRPVEQHLNVMTSPPQ---- 834

  Fly  1043 NAATEMFATNISMIKNSTLLIPNAKVAEASELSIYYKTTPLTPKQSHRSWSQSDLETPPRENFVH 1107
            .|.|                 |.|               |::|..:.|:
Zfish   835 GART-----------------PRA---------------PMSPMDTSRA---------------- 851

  Fly  1108 PTSNGDQTVLNETLADANKNPFNVELISSLLESIDFSMYIEKLPHCQLVG-HVKRLHPNTHLEVH 1171
                    |....:.|    |::.|||.|||.::  ...:...|:..:.. |:..:.|...:::.
Zfish   852 --------VCVPLVPD----PWDEELIVSLLSNL--QPPLSSQPNLTVWSCHLPSITPKMTVQIA 902

  Fly  1172 NEKFEVSKMIGKGAYGSVYVGKHLKSGKKVALKQERPTNYWEFYICLEIHSRL-TSEQMIPSYAH 1235
            .|...|..::|:||:.:||...:|.:.:|:.||.::|.|.|||||..:::.|| .||:.:  |..
Zfish   903 GESLRVDFVLGQGAFATVYQATNLMTTQKLFLKVQKPANPWEFYIDCQLNKRLQPSERHL--YNS 965

  Fly  1236 IDYALVGNNSSVYISEFSDYGSLIGVCNKVKSVTNRNMDEYVVMHLSCQMLDIVDHLHAMGIIHA 1300
            |..|.:.||.||.:.:..:.|:|:...|..|..:.:.|.:.:|::.|..:|.:|:.|||..||||
Zfish   966 IHSAHLFNNGSVLVGQLHNCGTLLNAINLYKRRSEKLMPQPLVLYFSVCILKMVELLHAAHIIHA 1030

  Fly  1301 DIKPDNFLL----MKPICADPNEV--SLQLIDFGVSIDMKLFPDNQTFNYVHHDDLFKCIEMRTG 1359
            |||||||||    ::..|.|...:  .|.|||.|.||||.|||:...|........|:|:||.:|
Zfish  1031 DIKPDNFLLGERFLENDCFDQENLQHGLALIDLGQSIDMTLFPEGTAFTAKCMTSGFQCVEMLSG 1095

  Fly  1360 RPWTYQLDLYGLVSVMHVLLFGRYMEVVQRSPSTIWMPKTNVPRYFQRTMWENIFRTLLNIRDCR 1424
            ||||||.|.:|:...::.:|||.||||.|.  ..:|.......|.....:|::.|.||||:.||.
Zfish  1096 RPWTYQTDYFGIAGTVYCMLFGTYMEVKQE--GDVWRTNGVFKRNVHADLWQDFFHTLLNVSDCS 1158

  Fly  1425 TMPNLQQLRTQLKCALAEKEKYVAEAISKFNTIL 1458
            ..|.|..||.:|...|  ::.|.::..|..|.::
Zfish  1159 APPCLSVLRQRLHAVL--QQNYNSKLRSLKNRLV 1190

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
BubR1	NP_477382.1	Mad3_BUB1_I	38..159	CDD:214817	38/114 (33%)
		STKc_Bub1_BubR1	1174..1458	CDD:270883	108/290 (37%)
bub1	XP_002665685.2	Mad3_BUB1_I	5..126	CDD:462420	38/115 (33%)
		Caldesmon	<146..>321	CDD:460421	47/314 (15%)
		Protein Kinases, catalytic domain	905..1189	CDD:473864	108/289 (37%)