DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Not3 and NOT3

DIOPT Version :9

Sequence 1:	NP_001260712.1	Gene:	Not3 / 35501	FlyBaseID:	FBgn0033029	Length:	844	Species:	Drosophila melanogaster
Sequence 2:	NP_012226.3	Gene:	NOT3 / 854773	SGDID:	S000001300	Length:	836	Species:	Saccharomyces cerevisiae

Alignment Length:	898	Identity:	228/898 - (25%)
Similarity:	385/898 - (42%)	Gaps:	175/898 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 ATRKLQGEIDRCLKKVAEGVETFEDIWKKVHNATNTNQKQKHLQEKYEADLKKEIKKLQRLRDQI 67
            |.||||.|:||..||:.||:|.|...:::..:.|| |..||   :|.|:|||:|:|||||||:||
Yeast     2 AHRKLQQEVDRVFKKINEGLEIFNSYYERHESCTN-NPSQK---DKLESDLKREVKKLQRLREQI 62

  Fly    68 KSWIASAEIKDKSSLLENRRLIETQMERFKVVERETKTKAYSKEGLGAAQKMDPAQRIKDDARNW 132
            |||.:|.:||||.|||:.||.:|..||::|.||:.:|.||||...|..::.:||.:|.:.|...:
Yeast    63 KSWQSSPDIKDKDSLLDYRRSVEIAMEKYKAVEKASKEKAYSNISLKKSETLDPQERERRDISEY 127

  Fly   133 LTSSISSLQIQIDQYESEIESLLA--GKKKRLDRDKQERMDDLRGKLDRHKFHITKLETLLRLLD 195
            |:..|..|:.|.|..:.||:.||.  .|||.......|:.:..:....|:::|..::|..||||.
Yeast   128 LSQMIDELERQYDSLQVEIDKLLLLNKKKKTSSTTNDEKKEQYKRFQARYRWHQQQMELALRLLA 192

  Fly   196 NDGVEAEQVNKIKDDVEYYIDSSQEPDFEENEFIYD-------DIIGLDEVELSGTATTDSNNSN 253
            |:.::.:.|..::||:.|:::|:|:|||.|:|.|||       :.|..:..:...:...:.||::
Yeast   193 NEELDPQDVKNVQDDINYFVESNQDPDFVEDETIYDGLNLQSNEAIAHEVAQYFASQNAEDNNTS 257

  Fly   254 ETSGSPSSVTSGGSPSQSPVTVQQILNTSSQGAASSGSSAASAALFQQQLTAAQSNGNNVGYASD 318
            :.:.|                :|.|...|.:........|..||    :|.|..:.|..:..|..
Yeast   258 DANES----------------LQDISKLSKKEQRKLEREAKKAA----KLAAKNATGAAIPVAGP 302

  Fly   319 TS-------AASSSATTSTDPAGGTVAVNCVGGLADKRN-KSSESNALKLKPQPHQLIKPTPVRA 375
            :|       .|.:|..|...|:...:........|.|.: ||..|:|..|.|.    ::.:|..|
Yeast   303 SSTPSPVIPVADASKETERSPSSSPIHNATKPEEAVKTSIKSPRSSADNLLPS----LQKSPSSA 363

  Fly   376 TAKLPLSSDTQV----NKIVSST-------PSKNQQQLPTAASIVATSAMQSQSSIGSCSS---- 425
            |.:.|.:..|.:    |.|..:|       |:|...:|..|  :.|:.|::....:.|.||
Yeast   364 TPETPTNVHTHIHQTPNGITGATTLKPATLPAKPAGELKWA--VAASQAVEKDRKVTSASSTISN 426

  Fly   426 ----TGGTGASQSASSGNNPG-----NNPAVQPNAPT--PGQSGIAAAAASTNVVSATIVSSANV 479
                |..|.|:.:.||..|..     |.|.:..::.:  |..:|.:::||:...|.|...::.:.
Yeast   427 TSTKTPTTAAATTTSSNANSRIGSALNTPKLSTSSLSLQPDNTGASSSAATAAAVLAAGAAAVHQ 491

  Fly   480 QGQSVIQ-------PTPTIAFAAVAKHN--TSLLENGP------VLQQQLAVTPTVAAIVGAGTQ 529
            ..|:..:       |..::|....::|.  |::.:|||      |::|:
Yeast   492 NNQAFYRNMSSSHHPLVSLATNPKSEHEVATTVNQNGPENTTKKVMEQK---------------- 540

  Fly   530 AQQKHVPPLSNLQTNSPHIQNGLPVSDSTNDNSCNVVDTISLKTMAQDAINRSAIDPNSLNQQQT 594
                        :..||..:|.|.|.      :..|.|.                |..|....:|
Yeast   541 ------------EEESPEERNKLQVP------TFGVFDD----------------DFESDRDSET 571

  Fly   595 SSIDLRQPQSQKSL-LQHFNSETNTNQQQLTSQQQQQLQNNSLAATTGSNNGTSTGSGLMNVANA 658
            ...:..||.:.|.| |:...::||..:::..|..:..|..:.:.....|       |.|.|  :.
Yeast   572 EPEEEEQPSTPKYLSLEQREAKTNEIKKEFVSDFETLLLPSGVQEFIMS-------SELYN--SQ 627

  Fly   659 TGQPISGNAKTHACQPQQMATTEAHIPTLLGVTPLGPTPLQK-EHQMQFQMMEAAYYHLPQPMDT 722
            ....|:.......|:..::    ..:|.  ||.|  |:||.. ....|:.:|..:.
Yeast   628 IESKITYKRSRDMCEISRL----VEVPQ--GVNP--PSPLDAFRSTQQWDVMRCSL--------- 675

  Fly   723 EKLQTYFHRAPVLTPSHYPQAQMPIYDTV-EFYQRLSTETLFFVFYYMEGSKAQYLAAKALKKQS 786
                    |..::......:....||..: |.::.|...:||:.:|:......:.:|.|.|.::.
Yeast   676 --------RDIIIGSERLKEDSSSIYAKILENFRTLEMFSLFYNYYFAITPLEREIAYKILNERD 732

  Fly   787 WRFHTKYMMWFQRHEEPKIINDDYEQGTYIYFDYEKWSQRKKEGFTFEYKYLE 839
            |:......|||.|..|.|..|:..|.|.|..|..:.|:...|..|..:|.:|:
Yeast   733 WKVSKDGTMWFLRQGEVKFFNEICEVGDYKIFKLDDWTVIDKINFRLDYSFLQ 785

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Not3	NP_001260712.1	Not3	3..233	CDD:281986	98/238 (41%)
		DUF3682	428..>509	CDD:289231	21/102 (21%)
		NOT2_3_5	711..836	CDD:282064	26/125 (21%)
NOT3	NP_012226.3	Not3	2..230	CDD:397951	98/231 (42%)
		PHA03247	<293..399	CDD:223021	25/109 (23%)
		NOT2_3_5	<691..782	CDD:398019	25/90 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	186	1.000	Domainoid score	I671
eggNOG	1	0.900	-	-		E1_COG5665
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0004227
OrthoInspector	1	1.000	-	-		oto99389
orthoMCL	1	0.900	-	-		OOG6_101346
Panther	1	1.100	-	-	O	PTHR23326
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R834
SonicParanoid	1	1.000	-	-		X2972
TreeFam	1	0.960	-	-
	9	8.890