Protein Alignment CG30440 and Arhgef25

DIOPT Version :9

• Sequence 1: NP_610172.1
• Gene: CG30440 / 35496
• FlyBaseID: FBgn0050440
• Length: 1057
• Species: Drosophila melanogaster
• Sequence 2: NP_082303.2
• Gene: Arhgef25 / 52666
• MGIID: 1277173
• Length: 618
• Species: Mus musculus
• Alignment Length: 504
• Identity: 150/504 - (29%)
• Similarity: 241/504 - (47%)
• Gaps: 79/504 - (15%)

  Fly   474 SEEDCCE-----CNEYQCIATPPNELSKDCLNAVEEGVFCNTEYDDFHENLTSSCCCSG------ 527
            :|.||.|     ..|.:.:|..|...|...  ||.||..             |:...||
Mouse    47 TESDCIEEDQTGQREPEVLAWAPQPESYSI--AVSEGSM-------------SASAVSGLAALSG 96

  Fly   528 --SFCDDDGCSYLDEGLSVDIK-----SK---NVQSLHESG---------------TDEKLSRKT 567
              |....|.||.:..|....::     ||   :|::..|||               |.::|...|
Mouse    97 PSSGLSSDPCSPIPPGPVTGLRRWLDHSKHCLSVETEAESGQTGQCENWTLEPTLTTGQELPELT 161

  Fly   568 ILLARTEDTNDVTQGISDGTTYFTGSLNITHLPKSNEIFKKPQDPEMQRHRRGHILKELFETEKI 632
            :|....|...|..|...:.|        ::..||:.|..||     |...|...:|.||.|||:.
Mouse   162 LLTTLLEGPGDKAQPAEEET--------LSQAPKNEEEQKK-----MALERSMFVLGELVETERT 213

  Fly   633 YVNEIASILKGYYDRLKSDESAPASLQGKANVLFGNLNEIYSFHNDVFLKDLENCISVTERVALC 697
            ||:::..|::||...: :.:..|.||:|:..::|||:.:||.:|.|.||::|:.|:...:.:|..
Mouse   214 YVDDLGQIVEGYMATM-ATQGVPESLRGRDRIVFGNIQQIYEWHRDYFLQELQQCLKDPDWLAQL 277

  Fly   698 FVKRRDKFYQLYSFYCQNIQRSEKLRETLVDTHMFFKECQIGLGHKLPLGAYLLKPVQRITKYQL 762
            |:|...:.: :|..||||..:||.:.....|:  :|:|.:..|||:|.|...|:||||||.||||
Mouse   278 FIKHERRLH-MYVVYCQNKPKSEHVLSEFGDS--YFEELRQQLGHRLQLNDLLIKPVQRIMKYQL 339

  Fly   763 LLKDLLLF---TDNDSCRNELKNALDCMLIVLKCVNDSMHQISITGFSGDLAKQGELLMQDSFQV 824
            ||||.|.:   ...|:  .||:.|::.|..|.|..||.|....:.||.|.|..||:||.||:|.|
Mouse   340 LLKDFLKYYRRAGKDT--EELEQAVEVMCFVPKRCNDMMSLGRLRGFEGKLTAQGKLLGQDTFLV 402

  Fly   825 WIESKKDIRLRMKPKRRHIFLYQKSLLLCKQTSKSGYN--KSSYQFKSDVKMSQIGLTESVSGDA 887
             .|.:....|..:.:.|.:||:::.::..:.....|..  :..|.:|:.:|:|.:||..::.|:.
Mouse   403 -TEPEAGGLLSSRGRERRVFLFEQIIIFSEALGGGGRGGAQPGYVYKNSIKVSCLGLEGNLQGNP 466

  Fly   888 KRFEVWLKGRQ---EVYTLQASTIGVKEMWVAEIKRVLFNQLEKLKGDQ 933
            .||.:..:|.:   :.|.||||...|.:.|:.::.::|.:|.:.|...|
Mouse   467 CRFALTSRGPEGGIQRYVLQASDPAVSQAWIKQVAQILESQRDFLNALQ 515

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG30440	NP_610172.1	RhoGEF	622..796	CDD:214619	67/176 (38%)
		PH-like	802..925	CDD:302622	36/127 (28%)
		PH	829..922	CDD:278594	22/97 (23%)
Arhgef25	NP_082303.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	26..63		5/15 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	169..193		6/31 (19%)
		RhoGEF	203..374	CDD:279015	67/176 (38%)
		Important for binding to Rho GTPases. /evidence=ECO:0000250	317..338		13/20 (65%)
		PH2_Kalirin_Trio_p63RhoGEF	378..520	CDD:270061	39/139 (28%)
		Sufficient to bind activated GNAQ. /evidence=ECO:0000250	506..532		3/10 (30%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	521..556
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	584..604

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0689
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810