Protein Alignment CG30440 and mcf2

DIOPT Version :10

• Sequence 1: NP_610172.1
• Gene: CG30440 / 35496
• FlyBaseID: FBgn0050440
• Length: 1057
• Species: Drosophila melanogaster
• Sequence 2: XP_031747179.1
• Gene: mcf2 / 100489667
• XenbaseID: XB-GENE-949776
• Length: 1118
• Species: Xenopus tropicalis
• Alignment Length: 1110
• Identity: 284/1110 - (25%)
• Similarity: 463/1110 - (41%)
• Gaps: 321/1110 - (28%)

  Fly    32 MTHYIHEGK------KAIMYRSSEPQIAARRNKKTRFVGNRRQLRNLSQPENSHKTSRSLCPDTA 90
            :.|||.|.:      ....||.|:..|.     :|...|....::.::|...|..|      :.|
 Frog   174 LLHYIDEKQLTPEFGGTFDYRHSDWVIC-----RTAIEGFALTVKEIAQRLQSFGT------ELA 227

  Fly    91 KTE-PK-VYSLRFVLNKSEDKDYMSRLKAQNDFSQSSCLCNNLSHANTEAGAYQNDG-----ILK 148
            :|| |: |||:..:|....:|.:..:....:...:.|.|.|:|            ||     :.:
 Frog   228 ETELPRDVYSIEHILAVRNEKYFQLKEGIMSVMREGSLLLNSL------------DGPTPTLVEE 280

  Fly   149 GQ--RDHSDFLTAAGGIQSRADRIARIETC--GIRTTHLQPETQILKPVTEPNETRFDQHSPDWL 209
            ||  ..:.|:.|    :|....::..:||.  |....|.....|.|:.:      :|:||
 Frog   281 GQHCEKNGDWET----VQRLLAQLHEMETAFDGFWEKHQLKMEQYLELL------KFEQH----- 330

  Fly   210 FKNSRIGDVSNSSKLSACNSELPPEEYKGRTLYHDTKFDCAYIKNRRPLITTESKMSITEAEHPQ 274
                 ..::.|.::......|..|:.....:.......|.:.::.|...:...|:|.|
 Frog   331 -----FEELKNGTEYLRSQHENIPDTGDNASEVRQRLMDLSNLEERSQELIRRSQMVI------- 383

  Fly   275 TKYTDIKYDYKTCSISQGLHQNKAFLNKVPPSKQSKPFHSVDYDYYENI------QKLHLSKESE 333
                              ||.::.               :|::.|..|:      :..|:|....
 Frog   384 ------------------LHGHQL---------------AVNHHYALNLICQQCNELRHMSDVLS 415

  Fly   334 RQIRDNGDPNDLQNFLKKNLIKATTNELLKAAAQSTKKCNIDLERKLNDLNIKEEDHTSNDSGNS 398
            .:|:            :|....:||.||.....|.|:.|                     |.|
 Frog   416 EEIK------------RKYAQLSTTLELHTRLQQVTECC---------------------DKG-- 445

  Fly   399 YKNDPYLVSMSKINSCKFIRSYNRDCETIFPTGDHS---DLNEFRK------SFMPQSVD----- 449
                .|.::..:::.|:            ...|.|.   ||.:|.:      ::.||:::
 Frog   446 ----AYFLANQQMDKCQ------------SKEGAHKALLDLEKFLENASPYSTYDPQTLEYEYES 494

  Fly   450 -LLSSASVSLSSCDGSCLNKDSICGSEEDCC------ECNEYQCIATPPNELS--KDCLNAVEEG 505
             |.......:.|......|...:..:.:.|.      :....|.:|..|...|  |..|.:.:.|
 Frog   495 ILTDELKTQIQSVHNKIENVRGLFENRQSCLKKLADKQARPVQLVAPRPETPSRAKSPLFSPKHG 559

  Fly   506 VFCNTEYDDFHENLTSSCCCSGSFCDDDGCSYLDEGLSVDI------------KSKNVQSLHESG 558
            :       ||:..|.                     .|.||            .::.::.:|:
 Frog   560 M-------DFNSTLK---------------------FSFDITLPGKRTTRKIQTTRKIEVMHD-- 594

  Fly   559 TDEKLSRKTILLARTEDTNDVTQGISDGTTYFTGSLNITHLPKSNEIFKKPQDPEMQRHRRGHIL 623
            ..|:.:...:.::.:||:.|:.                                      :||::
 Frog   595 YQERRNSLQLFISESEDSLDIL--------------------------------------KGHVI 621

  Fly   624 KELFETEKIYVNEIASILKGYYDRLKSD---ESAPASLQGKANVLFGNLNEIYSFHNDVFLKDLE 685
            .||.|||:|||.|:.::|.||...:::.   ...|.||:...|:||||:.|||.|||.|||:.||
 Frog   622 HELIETERIYVEELYTVLLGYRSEMENPAMMHFMPQSLRNMKNILFGNMPEIYEFHNKVFLQSLE 686

  Fly   686 NCISVTERVALCFVKRRDKFYQLYSFYCQNIQRSEKLRETLVDTHMFFKECQIGLGHKLPLGAYL 750
            :||...|:|..||::||:.| |:|..||||..||:.|.....|: :||:|||..|.|||.|.:||
 Frog   687 SCIEAPEKVGFCFLERREDF-QMYEKYCQNKPRSDSLWRQFSDS-VFFQECQKKLDHKLALDSYL 749

  Fly   751 LKPVQRITKYQLLLKDLLLFTDNDSCRNELKNALDCMLIVLKCVNDSMHQISITGFSGDLAKQGE 815
            ||||||:||||||:|:||.::.|.....||:.|||.||.:||.||||||||:|||::||:.:.|.
 Frog   750 LKPVQRLTKYQLLIKELLKYSSNADGAEELQEALDSMLDLLKSVNDSMHQIAITGYNGDINELGR 814

  Fly   816 LLMQDSFQVWIESKKDIR-----LRMKPKRRHIFLYQKSLLLCKQTSK--SGYNKS-SYQFKSDV 872
            :|||.||.|||..||...     .|.||.:||:|||:::|:.||:..:  .|::|: ||.||..:
 Frog   815 ILMQGSFSVWITHKKGATKMKELARFKPMQRHLFLYERALVFCKKREEHGDGHDKTPSYSFKHIL 879

  Fly   873 KMSQIGLTESVSGDAKRFEVWLKGRQEVYTLQASTIGVKEMWVAEIKRVLFNQLEKLKG------ 931
            ||:.:|:||:|.||:::||:|..||:|||.:||.|...|..|:.||:::|..|.|.:||
 Frog   880 KMNAVGITENVKGDSRKFEIWYSGREEVYIVQAQTSEQKTAWLNEIRKILTRQQEHMKGTVQKQK 944

  Fly   932 -----DQIARYNIRHQSLWQKSSCEAPNITHCSLRRTECFELPLNDSNRNTNIRSEKTCLSDQNI 991
                 ||::.....::...|:||..:..                |||..::.|..|...||.  :
 Frog   945 PNASTDQLSFSPQMNERKPQRSSVSSEE----------------NDSESSSPIMLEGLNLSP--L 991

  Fly   992 VNGP--------SVQDQHEKNSWSSD-----CSDIDEDETINPILMQKTGQPKLL--WNRSSSHQ 1041
            ...|        ||:.....:.||::     |||.:|::|:    ....|:.|.|  ..:..|..
 Frog   992 HKSPRGWTGMSQSVEICEGLDGWSNNPCSCPCSDTEEEDTV----QLSPGKYKALADCKKRGSED 1052

  Fly  1042 VFIKS 1046
            :.:|:
 Frog  1053 ILVKN 1057

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG30440	NP_610172.1	RhoGEF	622..796	CDD:214619	88/176 (50%)
		PH-like	802..925	CDD:473070	57/130 (44%)
mcf2	XP_031747179.1	SEC14	52..192	CDD:214706	4/17 (24%)
		SPEC	326..522	CDD:413338	41/296 (14%)
		RhoGEF	619..794	CDD:238091	88/176 (50%)
		PH-like	801..932	CDD:473070	57/130 (44%)
		SH3_DBS	1038..1092	CDD:212791	4/20 (20%)