Protein Alignment CG30440 and mcf2l.2

DIOPT Version :10

• Sequence 1: NP_610172.1
• Gene: CG30440 / 35496
• FlyBaseID: FBgn0050440
• Length: 1057
• Species: Drosophila melanogaster
• Sequence 2: XP_031757909.1
• Gene: mcf2l.2 / 100380200
• XenbaseID: XB-GENE-5959163
• Length: 1291
• Species: Xenopus tropicalis
• Alignment Length: 1028
• Identity: 295/1028 - (28%)
• Similarity: 436/1028 - (42%)
• Gaps: 285/1028 - (27%)

  Fly   147 LKGQRDHSDFLTAAGGI-----------QSRADRIARIETCGIRTT--HLQPETQILKPVTEPN- 197
            |.|..|.|...|..||.           ::..:..|::    ::||  :||.....|.....||
 Frog   228 LHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQV----VKTTAQNLQTFGTSLAETELPND 288

  Fly   198 ----ETRFDQHSPDWL-FKNSRIGDVSNSSKLSACNSE---------LPPEEYKGRTLY------ 242
                |.....|:...: .||.....:.....|.:|..|         |.|:|.:.....
 Frog   289 VLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQ 353

  Fly   243 --------------HDTKFD-CAYIKN-----RRPLITTESKMSITEAEHPQTKYTDI-----KY 282
                          |..|.: |..:::     |...:|.:..|.|      |..:.||     :.
 Frog   354 LDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEI------QAAFADIGDSVSRV 412

  Fly   283 DYKTCSISQGLHQNKAFLNKVPPSKQSKPFHSVDYDYYENIQKLHLSKESER----QIRDNGDPN 343
            :|....:.:...:.:..|.|.       ..|::..|  :.||..|.:.:|.|    ::|...|
 Frog   413 EYLIKELKKLEEKGQGMLEKA-------QLHALHGD--QLIQNNHYAVDSIRPKCIELRRICD-- 466

  Fly   344 DLQNFLKKNL-IKATTNELLK---------------AAAQSTKKCNID--LERKLNDLNIKEED- 389
            |..|..||.. |.|.:.||.:               .|:|:..||...  .|..|.|:.:..|.
 Frog   467 DFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESS 531

  Fly   390 ---HTSNDSGNSYKNDPYLVSMSKINSCKFIRS-------YN-----------RDCETIFPTGDH 433
               ..||.:....|.|..|....|.|..|.::.       :|           :....|.|...|
 Frog   532 RGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPH 596

  Fly   434 SDLNEFRKSFMPQSVDLLSSASVSLSSCDGSCLNKDSICGSEEDCCECNEYQCIATPPNELSKDC 498
            .:.:..|.|  |::| ..:|.|.:..:.|..|:                       ||..||:..
 Frog   597 PESSPKRSS--PKTV-RPTSLSTTRRAADVPCV-----------------------PPKSLSEAD 635

  Fly   499 LNAVEEGVFCNTEYDDFHENLTSSCCCSGSFCDDDGCSYLDEGLSVDIKSKNVQSLHES---GTD 560
            |:...            :...|.:                  |:.:::       :||:   ||.
 Frog   636 LSRKR------------NNKKTKA------------------GIKIEV-------MHEASQGGTS 663

  Fly   561 EKLSRKTILLARTEDTNDVTQGISDGTTYFTGSLNITHLPKSNEIFKKPQDPEMQRHRRGHILKE 625
                 :|::::.:|:                   |::                   .|:.||:.|
 Frog   664 -----RTLVMSESEE-------------------NLS-------------------SRKSHIINE 685

  Fly   626 LFETEKIYVNEIASILKGYYDRLKSDES---APASLQGKANVLFGNLNEIYSFHNDVFLKDLENC 687
            |.|||::||.|:.|||:||...|::.|.   .|.:||....||||||:|||.||..:|||:||||
 Frog   686 LIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENC 750

  Fly   688 ISVTERVALCFVKRRDKFYQLYSFYCQNIQRSEKLRETLVDTHMFFKECQIGLGHKLPLGAYLLK 752
            |...:.:..||:||::.. |:|..||||..|||.:.....:: :||:|||..|.|||.|.|||||
 Frog   751 IENADLLGTCFLKRKEDL-QIYEKYCQNKPRSEAIWRQCAES-IFFQECQRRLDHKLSLDAYLLK 813

  Fly   753 PVQRITKYQLLLKDLLLFTDNDSCRNELKNALDCMLIVLKCVNDSMHQISITGFSGDLAKQGELL 817
            |||||||||||||::|..:.|.....||:.||..:|.::|.||||||||:|||:.|||...|:||
 Frog   814 PVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLL 878

  Fly   818 MQDSFQVWIESK------KDIRLRMKPKRRHIFLYQKSLLLCK--QTSKSGYNKS-SYQFKSDVK 873
            ||.||.||.:.|      ||: .|.||.:||:|||.|:||.||  :.:..|:.|| ||.||..:|
 Frog   879 MQGSFNVWTDHKKGHNKVKDL-ARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILK 942

  Fly   874 MSQIGLTESVSGDAKRFEVWLKGRQEVYTLQASTIGVKEMWVAEIKRVLFNQLEKLKGDQIARYN 938
            ||.:|:||:|.||.|:||||..||:|||.:|||||.:|.:||:||::||..|||
 Frog   943 MSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLE----------- 996

  Fly   939 IRHQSLWQKSSCEAPNITHCSLRRTE-CFELPLNDSNRNTNIRSEKTCLSDQNIVNGPSVQDQHE 1002
                      :|...:..|  .|.|| .:..|:|.|   ...||.||..:.||....||::..::
 Frog   997 ----------ACREASQLH--QRITESVYHAPMNSS---FIARSNKTSGNSQNKGELPSLEVSNK 1046

  Fly  1003 KNSWSSDCSDIDEDETINP--------ILMQKTGQPKLLWNRSSSHQVFIKSK 1047
            ||..||...  .:.:..||        .|.:|....:.|.||.:.|:...:.|
 Frog  1047 KNRTSSPTG--RQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKAESQEK 1097

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG30440	NP_610172.1	RhoGEF	622..796	CDD:214619	88/176 (50%)
		PH-like	802..925	CDD:473070	69/131 (53%)
mcf2l.2	XP_031757909.1	SEC14	101..246	CDD:214706	7/17 (41%)
		SPEC	377..579	CDD:238103	44/218 (20%)
		RhoGEF	679..856	CDD:238091	88/178 (49%)
		PH_Dbs	863..994	CDD:269934	69/131 (53%)
		SH3_DBS	1188..1242	CDD:212791