Protein Alignment step and SYT1

DIOPT Version :10

• Sequence 1: NP_001036375.2
• Gene: step / 35425
• FlyBaseID: FBgn0086779
• Length: 727
• Species: Drosophila melanogaster
• Sequence 2: NP_015420.1
• Gene: SYT1 / 856210
• SGDID: S000006299
• Length: 1226
• Species: Saccharomyces cerevisiae
• Alignment Length: 804
• Identity: 171/804 - (21%)
• Similarity: 280/804 - (34%)
• Gaps: 251/804 - (31%)

  Fly    25 KKCSPGLGGSESVGRKQPLQRSCLDL---STTRVGCDG---CVSLEFFQSESPESRRSNSTFYVN 83
            |:|.|....|:....::.:.|  |||   |....|.:.   .|:......:.||...     :.:
Yeast    68 KECEPTKKHSKLSRIRRKMGR--LDLNFRSANEKGSEDDEILVAQHLRNGQDPEEMP-----FKS 125

  Fly    84 TQNVSS--AVFKDNRQPLLERGSGSDS-------------------SPSSVCVRCSCNSITKSGD 127
            ..|:.|  .|.|.:.:.:....||||:                   .|||       |.:::...
Yeast   126 ENNIDSIEKVPKPDGERVTLTSSGSDNVKRNSKHAPFIPVKPALEKFPSS-------NRLSRDYR 183

  Fly   128 RNETPTMATPKSDIKIALKPPPKPRSPSMAPYTSVAT---YNITKLAPDSPPPASPTLSTQTVNC 189
            :::.||:.  ..|..:...|.....|.|.:..:|.|.   :|.:|.|    ..||.:.|:.::
Yeast   184 KSQEPTLF--NGDRLVPTLPTVSRISTSSSVGSSTAASRYFNPSKRA----VVASSSSSSSSI-- 240

  Fly   190 SDVTTKVTEVQRLPCD-EPNTEL------ISKRTEYKHTTITTTTRTLIVSRPVELL--LNENGE 245
                 |...:..:|.| .|..||      ||||...:..           ||.|::.  :|:|.
Yeast   241 -----KFNSLHAIPLDATPQIELAKQQDEISKRRFGRRR-----------SRTVDVFDYINKNN- 288

  Fly   246 IIARRSPRKSRSNSLGRMLDVQNEIGTGGLFMEADPC-SKNSLPPPPYIDDMRFIDDSSN----- 304
             .|:..|..|.|:.: |.:|.:|   |..|..  ||. |:..|.|    ||...|.:.::
Yeast   289 -TAKNKPPLSPSSFI-RTIDEKN---TNSLMQ--DPMGSRGPLLP----DDANIISNDTDGAEAS 342

  Fly   305 -------SQSESERNSNIH-------------------------KTNKYTSKNNNND-----SKR 332
                   |:|.|:..|.:.                         .|:.|.:|:|.|.     |:|
Yeast   343 HPDHQVLSRSRSQSTSFVQGKGGKRKSIEDEGYHNKLGLPHGSGPTSVYNNKSNANSTITGMSRR 407

  Fly   333 ESQQSNLCESCRMLLDRNYINTEQSDNLVILR----RPSKQIKDELCEVVSEMEALDVPE---DC 390
            .|...|...|        ::|. :|.:|...|    :..:|::.:|...:.::..:..||   .|
Yeast   408 SSSIVNALSS--------FVNL-RSSSLSSSRQQHLQQQQQLQQKLDVSLEDLPPVPAPEFSDSC 463

  Fly   391 KHSNKDKQMSIGRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKND- 454
            |                                        .||.|.....| .||..|.||:|
Yeast   464 K----------------------------------------DFLIKLAPYGK-FIGIILTEKDDE 487

  Fly   455 FNEDVLKAFVA-LHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMETFAQRYCQLNPDI------ 512
            |.::.|...:. ..:|.|..|..|||:.|....||.|.|:|||::..|:..|.:.....
Yeast   488 FKKNCLNYLLTNCFEFKNDPLDIALRKLLMFLELPKETQQIDRLIMAFSFAYYKAQKSYSKKKGI 552

  Fly   513 ---FTNTDTCYVLSFAIIMLNTSLHNPSVKDKPTVDQFISM--NRGINNGGDLPRGLLESLYESI 572
               ::|.|..|.::|:::||:|...||:.|.|.|...|:.:  |...:.|.::|..:|...||::
Yeast   553 ECPWSNADQVYFIAFSLLMLHTDYFNPNNKSKMTKHDFVDLVHNDKYSGGNEIPMAVLTYFYENV 617

  Fly   573 RTE-----------PFKIPQDDG---NDLMHTFFNPDKEGWLWKQGGRYKSWKRRWFILNDNCLY 623
            ..:           |..:  ||.   .|...|.|.                     ..|:.|.:|
Yeast   618 TAKESPKFNYFLMSPMAL--DDSILDKDAFDTNFA---------------------ITLSSNSMY 659

  Fly   624 YFEYTTDKEPRG-IIPLENISVREIHDRSKPHCFELFATGGADIIKACKTDSEGKVVEGKHTVYR 687
               ...|...|| |||.| .|:..|.   .|....:.|:|.|....|....|....:.|.:
Yeast   660 ---SPIDMIKRGSIIPKE-ASLSPIF---YPLTNSISASGIAPSTAASCPPSTSGTINGAN---- 713

  Fly   688 MSAATEEDQQEWIKRLTQSISHNP 711
            :..|.....:.....::...|:||
Yeast   714 LGTANSNSNRPASNSISSYFSYNP 737

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
step	NP_001036375.2	Sec7	395..577	CDD:460178	47/205 (23%)
		PH_GRP1-like	592..710	CDD:269954	22/118 (19%)
SYT1	NP_015420.1	Sec7	443..622	CDD:214569	52/219 (24%)