Protein Alignment step and PIK3R3

DIOPT Version :9

• Sequence 1: NP_001036375.2
• Gene: step / 35425
• FlyBaseID: FBgn0086779
• Length: 727
• Species: Drosophila melanogaster
• Sequence 2: NP_001290357.1
• Gene: PIK3R3 / 8503
• HGNCID: 8981
• Length: 478
• Species: Homo sapiens
• Alignment Length: 584
• Identity: 97/584 - (16%)
• Similarity: 185/584 - (31%)
• Gaps: 190/584 - (32%)

  Fly   156 MAPYTSVATYNITKLAPDSPP-PASPTLSTQTVNCSDVTTKVTEVQRLPCDEPNTELISKRTEYK 219
            |.||::...:.|....|..|| |..|..|.......|.:..:.:.:....|....|:..|..:..
Human    36 MMPYSTELIFYIEMDPPALPPKPPKPMTSAVPNGMKDSSVSLQDAEWYWGDISREEVNDKLRDMP 100

  Fly   220 HTTITTTTRTLIVSRPVELLLNENGEIIARRSPRKSRSNSLGRMLDVQNEIGTGGLFMEADPCSK 284
            ..|......:..:.....|.|.:.|.               .:::.:.:..|..|.   :||.:.
Human   101 DGTFLVRDASTKMQGDYTLTLRKGGN---------------NKLIKIYHRDGKYGF---SDPLTF 147

  Fly   285 NSLPP-------------PPYIDDMRFIDDSSNSQSESERNSNIHKTNKYTSKNNNNDSKRESQQ 336
            ||:..             .|.:|.......|...|.:..:..||....|...:.::...::..:.
Human   148 NSVVELINHYHHESLAQYNPKLDVKLMYPVSRYQQDQLVKEDNIDAVGKKLQEYHSQYQEKSKEY 212

  Fly   337 SNLCESCRMLLDRNYINTEQSDNLVILRRPSKQIKDELCEVVSEMEALDVPEDCKHSNKDKQMSI 401
            ..|.|        .|..|.|.   :.::|.:.:..:|..::..        |.|....:..:..|
Human   213 DRLYE--------EYTRTSQE---IQMKRTAIEAFNETIKIFE--------EQCHTQEQHSKEYI 258

  Fly   402 GRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKAFVAL 466
            .|.:     ::|.|..:|..::.:|..             |:.:|:....|....:|:..
Human   259 ERFR-----REGNEKEIERIMMNYDKL-------------KSRLGEIHDSKMRLEQDLKN----- 300

  Fly   467 HDFTNLILVQALRQFLWSFRLPGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNT 531
                     |||           :.::||:.|.:       :.||              :|.|.
Human   301 ---------QAL-----------DNREIDKKMNS-------IKPD--------------LIQLR- 323

  Fly   532 SLHNPSVKDKPTVDQFISMNRGINNGGDLPRGLLESLYESIRTEP----FKIPQDDGNDLMHTFF 592
                 .::|:..|        .:|:.|...:.|  :::..|:.|.    :.|.::|.| |.|.
Human   324 -----KIRDQHLV--------WLNHKGVRQKRL--NVWLGIKNEDADENYFINEEDEN-LPHY-- 370

  Fly   593 NPDKEGWLWKQGGRYKSWKRRWFILNDNCLYYFEYTTDKEPRGIIPLENISVREIHDRSKPHCFE 657
              |::.|..:...|.::         ::.||       .:|.|..        .|.:.||..|:
Human   371 --DEKTWFVEDINRVQA---------EDLLY-------GKPDGAF--------LIRESSKKGCY- 408

  Fly   658 LFATGGADIIKACKTDSEGKVVEGKHTVYRMSA-----ATEEDQQEWIKRL------TQSISHN 710
                       ||...::|:|   ||.|...:|     |...:....:|.|      |..:.||
Human   409 -----------ACSVVADGEV---KHCVIYSTARGYGFAEPYNLYSSLKELVLHYQQTSLVQHN 458

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
step	NP_001036375.2	Sec7	397..577	CDD:279680	27/183 (15%)
		PH_GRP1-like	592..710	CDD:269954	23/128 (18%)
		PH	596..708	CDD:278594	22/122 (18%)
PIK3R3	NP_001290357.1	SH2_nSH2_p85_like	76..184	CDD:198195	17/125 (14%)
		PI3K_P85_iSH2	188..347	CDD:293063	37/257 (14%)
		iSH2_PIK3R3	189..349	CDD:214018	37/258 (14%)
		SH2_cSH2_p85_like	368..471	CDD:198184	26/134 (19%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910