DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment step and Psd

DIOPT Version :9

Sequence 1:	NP_001036375.2	Gene:	step / 35425	FlyBaseID:	FBgn0086779	Length:	727	Species:	Drosophila melanogaster
Sequence 2:	NP_001334383.1	Gene:	Psd / 73728	MGIID:	1920978	Length:	1025	Species:	Mus musculus

Alignment Length:	837	Identity:	184/837 - (21%)
Similarity:	288/837 - (34%)	Gaps:	232/837 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 LRRQPKNKKCSPGLGGSESVGRKQPLQRSCLDLSTTRVGCDGCVS--------------LEF--- 65
            ||.:.......|..|||...|.:.|   |...|...::|.||..|              :.|
Mouse   149 LRLEASTSDPLPAGGGSVLPGSRDP---SRGPLVPPQIGADGLYSSLPNGLGGTPEHLAMHFRGP 210

  Fly    66 -----------------FQSESPESRRSNSTFYVNTQNVSSAVFKDNRQPLLERGSGSDSSPSSV 113
                             ..|.:....|:...|:..:.:..|:..|...|.|..||.||.......
Mouse   211 ADTGFLNQGDTWSSPREVSSHAQRIARAKWEFFYGSLDAPSSGAKPPEQVLPSRGVGSKQGSGVA 275

  Fly   114 CVRCSCNSITKSGDRNETPTMATPKSDIKIAL-----------------------KPPPKPRSPS 155
            ..|.:..|.|   |.::.|.....::||...|                       :||....||.
Mouse   276 VGRAAKYSET---DLDKVPLRCYRETDIDEVLAEREEADSAIESQPSSEGPHGTAQPPASRPSPC 337

  Fly   156 MAPYTSVATYNITKLAPDSPPPASPTLSTQTVNCSDVTTKVTEVQR--LPCDE-PNTELISKRTE 217
            ..|.:|:.:.|....|...               .||..:|.|...  .|.|. |::.|:..
Mouse   338 PGPSSSLGSGNEDDEAGGE---------------EDVDDEVFEASEGARPGDHMPHSGLLKS--- 384

  Fly   218 YKHTTITTTTRTLIVSRPVELLLNENG------------EIIARRSPRKSRS-NSLGRMLDVQNE 269
                             ||..||..:.            |.|......|..| :||..:..:.:.
Mouse   385 -----------------PVPFLLGTSPSADGPDSFSCVFEAILESHRAKGTSYSSLASLEALASP 432

  Fly   270 IGTGGLF--MEADPCSKNSLPPPPYIDDMRFIDDSSNSQSESERNSNIHKTNKYTSKNNNNDSKR 332
            ..|...|  .|..|......|.||....:..::..|.:.|.::        ..:|       .:|
Mouse   433 GPTQSPFFTFEMPPQPPAPRPDPPAPAPLAPLEPDSGTSSAAD--------GPWT-------QRR 482

  Fly   333 ESQQSNLCESCRMLLDRNYI--NTEQSDNLVILRRPSKQIKDELCEVVSEMEA-LDVPEDCKHSN 394
            |.::|   ::...|..|..:  .:...|:..:...|....:..|.::||:.:: ||..|.....:
Mouse   483 EVEES---DAGATLAPRKELPSPSHSEDSFGLGAAPLGSSEPPLSQLVSDSDSELDSTERLALGS 544

  Fly   395 KDKQMSIGRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDV 459
            .| .:|.|:       |..:|          ..|.:|..||:.:|..|..:..:||:.|||::.|
Mouse   545 TD-TLSNGQ-------KADLE----------AAQRLAKRLYRLDGFRKADVARHLGKNNDFSKLV 591

  Fly   460 LKAFVALHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSF 524
            ...::....||.:.|.||||.||....|.||.|:.:|::..|:|||.|.||:..::.|..:.|:.
Mouse   592 AGEYLKFFVFTGMTLDQALRVFLKELALMGETQERERVLAHFSQRYFQCNPEALSSEDGAHTLTC 656

  Fly   525 AIIMLNTSLHNPSVKDKPTVDQFISMNRGINNGGDLPRGLLESLYESIRTEPFKIPQDDGNDLMH 589
            |:::|||.||..::..:.|...||....|:|:|||.||.||::||.||:.|..:...|: .:|..
Mouse   657 ALMLLNTDLHGHNIGKRMTCGDFIGNLEGLNDGGDFPRELLKALYSSIKNEKLQWAIDE-EELRR 720

  Fly   590 TF---------------------------FNPDKEGWLWKQG-----------------GRYKSW 610
            :.                           ..|:....::|.|                 |: :.|
Mouse   721 SLSELADPNPKVIKRVSGGSGSSSSPFLDLTPEPGAAVYKHGALVRKVHADPDCRKTPRGK-RGW 784

  Fly   611 KRRWFILNDNCLYY--FEYTTDK-----EPRGIIPLENISVREIHDRSK-PHCFELFATGGADII 667
            |....||....||.  .||...|     |.:..|.:.:.......|.|| ||.|.|..   ||
Mouse   785 KSFHGILKGMILYLQKEEYQPGKALSEAELKNAISIHHALATRASDYSKRPHVFYLRT---AD-- 844

  Fly   668 KACKTDSEGKVVEGKHTVYRMSAATEEDQQEWIKRL---TQSISHNPFYDILVQRKK 721
                           ..|:...|.:.|..|.||.|:   ....|..||...:..:||
Mouse   845 ---------------WRVFLFQAPSLEQMQSWITRINVVAAMFSAPPFPAAVSSQKK 886

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
step	NP_001036375.2	Sec7	397..577	CDD:279680	62/179 (35%)
		PH_GRP1-like	592..710	CDD:269954	32/145 (22%)
		PH	596..708	CDD:278594	30/139 (22%)
Psd	NP_001334383.1	PHA03247	<17..426	CDD:223021	60/317 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	67..96
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	154..195		12/43 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	250..277		8/26 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	307..401		20/128 (16%)
		Sec7	543..709	CDD:238100	63/183 (34%)
		PH_EFA6	754..878	CDD:270107	32/144 (22%)
		DUF812	<891..980	CDD:368550
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	977..1025
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5307
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810