DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment step and gbf1

DIOPT Version :9

Sequence 1:	NP_001036375.2	Gene:	step / 35425	FlyBaseID:	FBgn0086779	Length:	727	Species:	Drosophila melanogaster
Sequence 2:	XP_009305378.1	Gene:	gbf1 / 566350	ZFINID:	ZDB-GENE-081107-54	Length:	1862	Species:	Danio rerio

Alignment Length:	754	Identity:	166/754 - (22%)
Similarity:	277/754 - (36%)	Gaps:	244/754 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 SNWFSSLR--RQPKNKKCSPGLGGSESVGRKQPLQRSCLDLSTTRVGCDGCVSLEFFQSESPESR 74
            |.|....|  |.|::...||.       |:..|.|.|.|
Zfish   237 SKWKKQKRSPRPPRHMVRSPS-------GQMDPAQSSTL-------------------------- 268

  Fly    75 RSNSTFYVNTQNVSSAVFKDNRQPLLERGS-------GSDSSPSSVCVRCSCNSITKSG-DRNET 131
                     |.|:|..|      |.:|:.|       ||||:.||:     .:..|.|| |....
Zfish   269 ---------TNNLSGGV------PFIEQHSGGSSTFPGSDSAASSI-----SSPTTDSGLDTGSK 313

  Fly   132 PTMATPKSDIKIALKPPPKPRSPSMAP--------------YTSVATYNITKLAPD--------- 173
            .|.....:|:.........|.:||..|              ..|.:..:|.::..|
Zfish   314 TTSKEDLTDLDQNSSTATTPITPSAEPGRLESQSEGRGVERAQSASVESIPEVLEDRDLLADQSD 378

  Fly   174 ----------SPPPASPTLSTQTVNCSDVTTKVTEVQRL---------PCDEPNT--------EL 211
                      :|.....|.|||....:.:...:..::.|         |.|..|:        :|
Zfish   379 SASVHDMDYVNPRGVRFTQSTQKEGAALIPYGLPCLRELFRFLISLTNPHDRHNSDVMMHMGLQL 443

  Fly   212 ISKRTEYKH----------------------------TTITTTTRTLI---------VSRPVELL 239
            ::...|..|                            ...|.:.|...         :...:|:.
Zfish   444 LNVALEAAHIAPYQSLLGLVKDELCRHLIQLLGVDRMNLYTASIRVCFLLFESMREHLKFQLEMY 508

  Fly   240 LNENGEIIARRSPR---KSRSNSLGRMLDVQN--------------EIGTGGLFMEADP-CSKNS 286
            |.:..:||...:|:   :.:..:|..::.:..              :.....||.:... .|||:
Zfish   509 LKKLMDIITSENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDFYCSNLFEDLTKLLSKNA 573

  Fly   287 LPPPPY--------IDDMRFIDDSS---------NSQSESERN----------SNIHKTNKYTSK 324
            .|....        ::.:..:.||:         ||.::.|::          |.|..:...|.|
Zfish   574 FPVSGQLYTTHLLSLEALLTVIDSTEAHCQAKVLNSATQQEQSESTTVGDSSVSTITDSPAETGK 638

  Fly   325 NNNNDSKRESQQSNLCESC----------RMLLDRNYINTEQSD--NLVILRRP---SKQIKD-- 372
            .:.:.:.:.|..|....||          :|.|.|.  :.|::|  ...|.::|   |..:.|
Zfish   639 PHPSSNGQNSVVSETRASCPPTSGHLMAEKMRLGRQ--DQEETDTGEKKISKKPHRFSSYLPDSQ 701

  Fly   373 ELCEVVSEMEALDVPEDCKHSNKDKQMSIGRKKFNMDPKKGIEYLVENRLLRH--DPQDVAHFLY 435
            ||.|:               .||.|.:..|.::||..|||||:.|.|..||..  |..:||.:|.
Zfish   702 ELLEI---------------KNKKKLLITGTEQFNQKPKKGIQTLQEKGLLSSPMDNNEVAQWLR 751

  Fly   436 KGEGLNKTAIGDYLGEKNDFNEDVLKAFVALHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMET 500
            :...|:|..||:::.::.  |.|:|.:||....|..|.:.:|||.:|.:|||||||..|.|::||
Zfish   752 ENPRLDKKMIGEFISDRR--NTDLLDSFVNTFTFQGLRIDEALRLYLEAFRLPGEAPVIHRLLET 814

  Fly   501 FAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKDK---PTVDQFISMNRGINNGGDLPR 562
            |...:.::|.:.|...|..:.|::|:|||||..||.:|:.:   .|::||....:|:|.|.|..:
Zfish   815 FTDNWHKVNGNPFQTNDAGFALAYAVIMLNTDQHNHNVRKQNIPMTLEQFKKNLKGVNGGNDFDQ 879

  Fly   563 GLLESLYESIRTEPFKIPQDDGNDLMHTFFNPDKEGWLW 601
            .:||.:|.:|:.|...:| |:...|:       ||.::|
Zfish   880 DMLEDIYNAIKNEEIVMP-DEQTGLV-------KENYVW 910

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
step	NP_001036375.2	Sec7	397..577	CDD:279680	69/184 (38%)
		PH_GRP1-like	592..710	CDD:269954	3/10 (30%)
		PH	596..708	CDD:278594	3/6 (50%)
gbf1	XP_009305378.1	Sec7_N	410..555	CDD:289549	15/144 (10%)
		COG5307	493..>1170	CDD:227623	114/445 (26%)
		Sec7	709..894	CDD:279680	70/186 (38%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5307
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D815698at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.820