DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment step and plekhh1

DIOPT Version :9

Sequence 1:	NP_001036375.2	Gene:	step / 35425	FlyBaseID:	FBgn0086779	Length:	727	Species:	Drosophila melanogaster
Sequence 2:	NP_001070987.1	Gene:	plekhh1 / 565914	ZFINID:	ZDB-GENE-061219-1	Length:	1433	Species:	Danio rerio

Alignment Length:	748	Identity:	140/748 - (18%)
Similarity:	221/748 - (29%)	Gaps:	299/748 - (39%)

- Green bases have known domain annotations that are detailed below.

  Fly    42 PLQRSCLDLSTTRV------------GCDGCVS----LEFFQ--SESPESRR----SNSTFYVNT 84
            |.|..|..|...|:            |||....    |...|  ::||:|.:    :.||...:.
Zfish   205 PAQEDCWRLPGCRLNSSADSKRKITEGCDSAFHSASLLPMLQDKADSPKSSQDGVDATSTVKESA 269

  Fly    85 QNV--SSAVFKDNRQPLLERGSGSDSSPS---------SVCVRCSCNSITKSGDRNETP------ 132
            :.|  |..|.:|...     |..||...|         |.|:|.|.:|.:.|....|||
Zfish   270 EGVEPSFGVMRDRAM-----GGASDRDHSSDELNSKFRSQCLRSSSSSSSSSSSAYETPGPGGFD 329

  Fly   133 -TMATPKSDIKIALKPP----------------------PKPRSPSMAPYTSVATYNITKLAPDS 174
             ...||||...::|.||                      ||.|:| :.|..|:   .:.|.....
Zfish   330 TPTPTPKSPPPVSLSPPLTRLPLSCPFPLALPLGTSMTLPKVRTP-LTPRDSI---QLVKKHHSQ 390

  Fly   175 PPPASPTLSTQTVNCS-DVTTKVTEVQRLPCDEPNTELISKRTEYKH----TTITTTTRTLIVSR 234
            |.|....|  ..||.| |:..         |..|:...:...:...|    |.|...|
Zfish   391 PQPGLERL--HQVNVSIDIGN---------CGSPSCHSLVPGSAPSHGLEETDIDEGT------- 437

  Fly   235 PVELLLNENGEIIARRSPRKSRSNSLGRMLDVQNEIGTGGLFMEADPCSKNSL-PPPPYIDDMRF 298
                  .|:.|::...|.......:.|.:||        ||..|.:.....:| ||.|.:.  ||
Zfish   438 ------PESMEVVVEGSEITEAQPNDGDLLD--------GLTKELEMMDPEALKPPTPPLH--RF 486

  Fly   299 IDDSSNSQSESERNSNIHKTNKYTSKNNNNDSKRESQQSNLCESCRMLLDRNYINTEQSDNL--- 360
                      ....|.|:...|...:              :.|:|........::|:...:.
Zfish   487 ----------PSWESRIYAVAKLGMR--------------VSEACLGAKTIGRVSTQPQHSTTGP 527

  Fly   361 ---VILRRPSKQIKDELCEVVSEMEALDVPEDCKHSNKDKQMSIGRKKFNMDPKKGIEYLVENRL 422
               :|.:..|..:...|....:::.::.:|:|...|..|...........:.|.|
Zfish   528 FTHLIYKNISVPVYSTLKGKATQISSVPLPDDDSGSEDDSSSLASLHTSTLGPDK---------- 582

  Fly   423 LRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKAFVALHDFTNLILVQALRQFLWSFRL 487
                         ||.                                                .
Zfish   583 -------------KGS------------------------------------------------T 586

  Fly   488 PGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKDKPTVDQFISMNR 552
            ||..:.:.|.:.|.:           .::::.|.:......|::....|..|.:.|
Zfish   587 PGSPRAVKRGVSTSS-----------ISSESDYAIPPDAYSLDSDCSEPEHKVQRT--------- 631

  Fly   553 GINNGGDLPRGLLESLY--ESIRTEPFKIPQDDGNDLMHTFFNPDKEGWLWKQGGRYKSWKRRWF 615
                          |.|  ||:..|..                 :|.|:|.|.|.:.|:||||||
Zfish   632 --------------SSYSCESVGPETL-----------------EKTGYLLKMGSQVKAWKRRWF 665

  Fly   616 IL-NDNCLYYFEYTTDKEPRGIIPLENISVREIHDRSKPHCFELFATGGADIIKACK-TDSEG-- 676
            || |...|||      |.|..:|             .||.       |..::..:|. ...||
Zfish   666 ILRNGEILYY------KSPSDVI-------------RKPQ-------GQMELNSSCHIARGEGAQ 704

  Fly   677 --KVVEGKHTVYRMSAATEEDQQEWIKRLTQSI 707
              :::..|.|.| ::|.:....::|| |:.|::
Zfish   705 TFQLITEKKTFY-LAADSPNILEDWI-RVLQNV 735

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
step	NP_001036375.2	Sec7	397..577	CDD:279680	18/181 (10%)
		PH_GRP1-like	592..710	CDD:269954	35/122 (29%)
		PH	596..708	CDD:278594	35/118 (30%)
plekhh1	NP_001070987.1	GBP_C	<47..156	CDD:303769
		coiled coil	145..156	CDD:293879
		PH	644..737	CDD:278594	35/137 (26%)
		PH1_PLEKHH1_PLEKHH2	646..741	CDD:241436	35/118 (30%)
		PH	755..854	CDD:214574
		MyTH4	896..1050	CDD:214535
		B41	1063..1293	CDD:214604
		FERM_M	1168..1293	CDD:278785
		FERM_C-lobe_PLEKHH1_PLEKHH2	1289..1390	CDD:241360
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910