DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment step and rasgrf2b

DIOPT Version :9

Sequence 1:	NP_001036375.2	Gene:	step / 35425	FlyBaseID:	FBgn0086779	Length:	727	Species:	Drosophila melanogaster
Sequence 2:	XP_009300046.1	Gene:	rasgrf2b / 553520	ZFINID:	ZDB-GENE-060504-1	Length:	1275	Species:	Danio rerio

Alignment Length:	471	Identity:	96/471 - (20%)
Similarity:	168/471 - (35%)	Gaps:	116/471 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    47 CLD--LSTTRVGCDGCVSLEFFQSESPESRRSNSTFYVNTQNVSSAVFKDNRQPLLERGSGSD-- 107
            |.|  |...::..|...|||.              |:...||..|....:::.|.|.|...|.
Zfish   707 CPDYSLKIRKIALDQSKSLEL--------------FFATNQNNRSGDHVNDKSPRLCRKFSSPPP 757

  Fly   108 ---SSPSSVCVR---CSCNS-----------ITKSGDRNETPTMATPKSDIKIALKPPPKPRSPS 155
               ||.:|..||   .|.||           .|.|.....:||.|.|      .:.|||...:
Zfish   758 LSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANP------TISPPPSNNN-- 814

  Fly   156 MAPYTSVATYNITKLAPDSPPPASPTLSTQTVNCSDVTTKVTEVQRLPCDEPNTELISKRTEYKH 220
                      |.:|...|.....||:...|:....|...:|..:..| |.:       .|...:.
Zfish   815 ----------NNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDAL-CGK-------LRRSIRR 861

  Fly   221 TTITTTTRTLIVSRPVELLLNENGEIIARRSPRKSRSNSLGRMLDVQN------EIGTGGLFMEA 279
            ..:.:.:....:  |.....:|.|||...|||...| :...|...||.      .:.....|..|
Zfish   862 AVLESVSLDKFI--PESPQASEPGEISPCRSPSTPR-HLRYRQSGVQTAENSRCSVSPASAFAIA 923

  Fly   280 DPCSKNSLPPPPYIDDMRFIDDSSNSQSESERNSNIHK--TNK-------YTSKNNNN-DSKRES 334
            ...:.:|.||.           .:||:...::...|.:  ||:       :.||::.: :...|.
Zfish   924 TAAAGHSSPPV-----------FNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGEL 977

  Fly   335 QQSNLCESCRMLLDRNYINTEQSDNLVILRRPSKQIKDELCEVVSEMEALDVPE--DCKHSNKDK 397
            :...:|....:|.|.:.:..|:.....||...|:..:|:     ::::..|:.:  :|..:...:
Zfish   978 KMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDD-----AQLKIEDILQMAECPKAECFE 1037

  Fly   398 QMSIGRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKA 462
            .:|.      |:..:.|. |:::.:.|..|.:  .||  |:|..||       :|.:....::|.
Zfish  1038 SLSA------MEIAEQIT-LLDHIVFRSIPYE--EFL--GQGWMKT-------DKTERTPYIMKT 1084

  Fly   463 FVALHDFTNLILVQAL 478
            ....:|.:||:..|.:
Zfish  1085 SQHFNDMSNLVASQIM 1100

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
step	NP_001036375.2	Sec7	397..577	CDD:279680	18/82 (22%)
		PH_GRP1-like	592..710	CDD:269954
		PH	596..708	CDD:278594
rasgrf2b	XP_009300046.1	PH_RasGRF1_2	19..154	CDD:270081
		PH	24..129	CDD:278594
		RhoGEF	243..424	CDD:279015
		PH	476..584	CDD:214574
		PH	476..583	CDD:278594
		RasGEF_N	634..>682	CDD:279012
		REM	<938..1010	CDD:295342	13/71 (18%)
		RasGEF	1036..1272	CDD:214539	18/83 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910