DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment step and APPL1

DIOPT Version :9

Sequence 1:	NP_001036375.2	Gene:	step / 35425	FlyBaseID:	FBgn0086779	Length:	727	Species:	Drosophila melanogaster
Sequence 2:	NP_036228.1	Gene:	APPL1 / 26060	HGNCID:	24035	Length:	709	Species:	Homo sapiens

Alignment Length:	441	Identity:	76/441 - (17%)
Similarity:	160/441 - (36%)	Gaps:	143/441 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   307 SESERNSNIHKTNK----YTSKNNNNDSKRESQQSNL---------CESCRMLLDRNYINTEQSD 358
            :::|.::..|.|:|    |..:........|...|.|         ..||..:|     :|:.:|
Human    50 AQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVL-----STQLAD 109

  Fly   359 NLVILRRPSKQIKD-ELCEVVSEMEALDVPEDCKHSNKDKQMSIGRKKFNMDPKKGIEYLVENRL 422
            .::.   |..|.|: :|.|:::..|...:      ::.|...:|.|  ::...||.     ||..
Human   110 AMMF---PITQFKERDLKEILTLKEVFQI------ASNDHDAAINR--YSRLSKKR-----ENDK 158

  Fly   423 LRHD-PQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKAFVALHDF---TNLILVQALRQFLW 483
            :::: .:||                 |...|.. ::.::..|.||:..   ..:.|::.|..::.
Human   159 VKYEVTEDV-----------------YTSRKKQ-HQTMMHYFCALNTLQYKKKIALLEPLLGYMQ 205

  Fly   484 S----FRLPGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKDKPTV 544
            :    |::..|  .::..:|.|                        :..:.||:.|
Human   206 AQISFFKMGSE--NLNEQLEEF------------------------LANIGTSVQN--------- 235

  Fly   545 DQFISMNRGINNGGDLPRGLLESLYESIRTEPFKIPQDDGNDLMHTFFNPDKEGWL---WKQGGR 606
                 :.|.:::..:..:..:|.|  .:.::|..:|..|............|.|:|   .|.|..
Human   236 -----VRREMDSDIETMQQTIEDL--EVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLV 293

  Fly   607 YKSWKRRWFILNDNCLYYFEYTTDKEPRG------IIPLENISVREIHDRSKPHCFELFATGGAD 665
            ..:|.|:::......|.       .:.||      .:.::|.||..:....:.:||::
Human   294 SSTWDRQFYFTQGGNLM-------SQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQI------- 344

  Fly   666 IIKACKTDSEGKVVEGKHTVYRMSAATEEDQQEW---IKRLTQSI--SHNP 711
                  |..:||    |.::  :.|.:::|.:||   |..:::.|  |.||
Human   345 ------TSFDGK----KSSI--LQAESKKDHEEWICTINNISKQIYLSENP 383

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
step	NP_001036375.2	Sec7	397..577	CDD:279680	25/187 (13%)
		PH_GRP1-like	592..710	CDD:269954	26/131 (20%)
		PH	596..708	CDD:278594	25/125 (20%)
APPL1	NP_036228.1	Required for RAB5A binding	1..428		76/441 (17%)
		BAR_APPL1	20..234	CDD:153315	41/248 (17%)
		BAR-PH_APPL	252..376	CDD:270067	29/151 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	397..434
		F&H	403..414
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	467..491
		PTB_APPL	490..625	CDD:269980
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	645..709
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910