DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nolo and Thsd7a

DIOPT Version :9

Sequence 1:	NP_001036374.1	Gene:	nolo / 35424	FlyBaseID:	FBgn0051619	Length:	1394	Species:	Drosophila melanogaster
Sequence 2:	NP_001178899.2	Gene:	Thsd7a / 500032	RGDID:	1566201	Length:	1645	Species:	Rattus norvegicus

Alignment Length:	1637	Identity:	318/1637 - (19%)
Similarity:	480/1637 - (29%)	Gaps:	648/1637 - (39%)

- Green bases have known domain annotations that are detailed below.

  Fly    79 SSWSDWSTCSRTCDGGIMHQMRRCGSP----GSCRGESTRYRICNMQPCPEQQDFRS------SQ 133
            |.:|.||.|||||..|:.|:.|...:|    ||.....|.:::|...||..::...|      |.
  Rat   187 SEFSSWSECSRTCGSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSNPCEAEESMYSLQVGPWSA 251

  Fly   134 CSAYNDVPYDGTLYKWTPHYDYVEPCALTCRGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQL 198
            ||              .||.........  ||......::..:...|..|.|            |
  Rat   252 CS--------------VPHSRQARQARR--RGKNKEREKERGKAVKDPEARE------------L 288

  Fly   199 SARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSCSQPLFNWEMAPMSQ 263
            ..:.::..|          |.:.:....|::|
  Rat   289 IKKKRNRNR----------QNRQENRYWDIQI--------------------------------- 310

  Fly   264 CSVTCGSGYKMSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCP-PRWIADDWSTCSRLC 327
                   ||:....:|.|| |....|.:.|.....|........|..|. ..|  .:||.||:.|
  Rat   311 -------GYQTRDVMCLNR-TGKSADLSFCQQERLPMTFQSCVITKECQVSEW--SEWSPCSKTC 365

  Fly   328 GHGYRERMVVCAEESNGIKTRV-------ADIMCRTPKPPTQETCIIEE-----CP--HWEVEDW 378
             |.      |.:.....::||.       ::..|  |:...:|.|:.:.     |.  .|...:|
  Rat   366 -HD------VTSPTGTRVRTRTIRQFPIGSEKEC--PELEEKEPCLSQGDGAVLCATYGWRTTEW 421

  Fly   379 TGCSVS----------------CGQGIQMRGVECKSTDGSL-----SAKCDPLTKPGSMQQCSTG 422
            |.|.|.                ||.|:|.|.:.|..|:..|     :.|....:||...:.| ||
  Rat   422 TECHVDPLLSQQDKRRANQTALCGGGVQTREIYCIQTNDILLSHINTQKDKEASKPVDSKLC-TG 485

  Fly   423 --------------IHCGGSLNKVGGTIIVGSSRSLNERSERQLDSSDADEDNEDEND-EGDDVD 472
                          |.|..|.....|....                       |:.|| :|....
  Rat   486 PVPNTTQLCHVPCPIECEVSPWSAWGPCTY-----------------------ENCNDQQGKKGF 527

  Fly   473 DLESGQDTDDGEGLSYA-------------DQPLLYAHRTQSRLNQEAPDEPRTMHLMNGNSNNN 524
            .|...:.|::..|.|.|             ::|..|..:: .||....||        ||.:...
  Rat   528 KLRKRRITNEPTGGSGATGNCPHLLEAIPCEEPSCYDWKS-VRLGDCEPD--------NGKACGP 583

  Fly   525 FNRGED----ESEGPSLDPTYIKD----------------------NEWSPCSVTC------GEG 557
            ..:.::    .|:|..:|....:|                      :.||.||.||      |:.
  Rat   584 GTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAPCPKDCVLSAWSTWSSCSHTCSGKTTEGKQ 648

  Fly   558 IRRRTYNCKIFLEYSRTVATVNDSLCEGKKPHDEVERCVEDPCMLPSHGFDDQFPRDSIKVGVSE 622
            ||.|:     .|.|:.....:.   |.......||..|.|.||.: .|.....:.:......||.
  Rat   649 IRARS-----ILAYAGEEGGIR---CPNSSALQEVRSCNEHPCTV-YHWQTGPWGQCIEDTSVSS 704

  Fly   623 PGKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEARVRTCNDRPC--- 684
            ...|..|  .|.||||.    |::...:.|||.:.|: |.|..|....:||. ||.|. .||
  Rat   705 FNTTTTW--TGETSCSV----GMQTRKVICVRVNVGQ-VGPKKCPESLRPET-VRPCL-LPCRKD 760

  Fly   685 ---PPRWNYSDYTPCSKSC-----GIGIKTREVQCIHEVTRGGDNTMVVPNSMCPQP-------- 733
               .|   |||:|||..||     |...::|:...|.....||..        |..|
  Rat   761 CIMTP---YSDWTPCPSSCREGDSGARKQSRQRVIIQLPAYGGRE--------CSDPLYEEKACE 814

  Fly   734 -PPADRQYCNVLDCPVRWEVGEWSKC--------------SHTCGYGFKDRKVECKQIMAQEHKI 783
             ||....|        ||:..:|.:|              ...||.|.:.|.:.|::....:..|
  Rat   815 APPTCHSY--------RWKTHKWRRCQLVPWSIQQDVPGAQEGCGPGRQARAITCRKQDGGQASI 871

  Fly   784 ERPESMC---PSAKPADKKPCNVKPCPPEDPKPVIQINNSTHIQHDPKKT---KITLKVGGAGLV 842
            :.    |   ....||..:.|.: ||                 |.|.:.|   |.:...|..|.|
  Rat   872 QE----CLQYAGPVPALTQACQI-PC-----------------QDDCQFTSWSKFSSCNGDCGAV 914

  Fly   843 ------FFGTQVKIK---------------CPVKRYNRTKI-KWSK------------------D 867
                  ..|...|.:               ||..:||...: .||.                  |
  Rat   915 RTRKRAIVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKAEVLLGVKVQGD 979

  Fly   868 HKPLQRSRKYKV----SKKGALRILDIT-------FRDAGVYSCHAGLSSAEIS----------- 910
            :|...:..:|:.    .:.|  |:::.:       ..:|.:..|.:....:|.|
  Rat   980 NKECGQGYRYQAMACYDQNG--RLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGS 1042

  Fly   911 -IEVKAK-----------PGQQIEELEQQETERLVREKSG-------TE-----ALTSADMKSAD 951
             ::|::|           |..:::.:.|.:...:|...|.       ||     .:|..||:...
  Rat  1043 GVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYIWVTEPWSVCKVTFVDMRDNC 1107

  Fly   952 GTGT---------STAVGPNSHVN-----------GSAQ-------------------------Q 971
            |.|.         :||.||:.||.           ||.:                         .
  Rat  1108 GEGVQTRKVRCMQNTADGPSEHVEDYLCDPEDMPLGSRECKLPCPEDCVISEWGPWTQCSLPCNP 1172

  Fly   972 HGSRRRQQQSERLQNGRERSRRPKSDGVQHADSSIMEDDLSQLVQSEDRIPQPASASSGGSRTRT 1036
            .|||:|         ..:..|:|..:|....|:          |:.|     |.:.:....|
  Rat  1173 SGSRQR---------SADPIRQPADEGRACPDA----------VEKE-----PCNLNKNCYR--- 1210

  Fly  1037 LLAMPYFQALLSNLQLLWPLQRFKDSRGQHLLQGEALKFG----IDLSGKDFDQDESERVPAEQL 1097
               ..|.....|..||           .:..:.|..:|..    :...||..|....|.:..|:
  Rat  1211 ---YDYNVTDWSTCQL-----------SEKAVCGNGIKTRMLDCVRSDGKSVDLKYCEELGLEK- 1260

  Fly  1098 PPEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGAAASTTAVPSLPNDGFVYRWMLGEW 1162
                                               .|:..::.:.    ..|.:..:..|  ..|
  Rat  1261 -----------------------------------NWQMNSSCTV----ECPLNCQLSDW--SPW 1284

  Fly  1163 SQCSQECGAAGSGLQRRTVSCQRAAKATSSGSTSDTENNVVDNSECFAHGLELPEFFQSCGNEAC 1227
            |:|||.||..|..::||||                |:....|...|    ..|.|..:.|..:.|
  Rat  1285 SECSQTCGLTGKMIRRRTV----------------TQPFQGDGRPC----PSLMEQSKPCPVKPC 1329

  Fly  1228 PQWSKGDWTPC--QRSHCHGRNTAMQRREVTCRYPNGTEGSICDEYERPAMRQECYN-------- 1282
            .:|..|.|:||  |.:.| |..|  :.|.::|...:|:    .|::.:....:.|.|
  Rat  1330 YRWQYGQWSPCQVQEAQC-GEGT--RTRNISCVVSDGS----ADDFSKAVDEEFCANIELIIDGN 1387

  Fly  1283 ----------ERCEGVWRVEPWSE---CNAPC------GRKGIKYR------------------I 1310
                      :.|.|...:..||.   |...|      |..||:.|                  |
  Rat  1388 KQIVLEETCTQPCPGDCYLNDWSSWSLCQLTCVNGEDLGFGGIQVRSRAVIIQELENQHLCPEQI 1452

  Fly  1311 L--------QC--------VWYGSRRPAG-------NV---CKHQPRPAVMKVCKSPACQAQLSS 1349
            |        ||        .|.||.|...       ||   |....:|...:.|..|..|.....
  Rat  1453 LETKSCDDGQCYEYKWMASAWKGSSRTVWCQRSDGVNVTGGCLVVSQPDADRSCNPPCSQPHSYC 1517

  Fly  1350 SPAQLCRDSSRY 1361
            |..:.||....|
  Rat  1518 SEMKACRCEEGY 1529

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nolo	NP_001036374.1	TSP1	78..124	CDD:214559	18/48 (38%)
		TSP1	546..600	CDD:214559	17/59 (29%)
		TSP1	750..807	CDD:214559	14/73 (19%)
		IGc2	845..902	CDD:197706	15/101 (15%)
		PLAC	1355..1384	CDD:285849	3/7 (43%)
Thsd7a	NP_001178899.2	TSP1_spondin	184..235	CDD:408798	17/47 (36%)
		TSP1_spondin	350..401	CDD:408798	14/61 (23%)
		TSP1_spondin	624..683	CDD:408798	17/66 (26%)
		TSP1_ADAMTS	688..757	CDD:408800	22/77 (29%)
		TSP1_spondin	1025..>1065	CDD:408798	5/39 (13%)
		TSP1_ADAMTS	1088..1151	CDD:408800	15/62 (24%)
		TSP1_spondin	1155..1206	CDD:408798	11/74 (15%)
		TSP1_spondin	1276..1329	CDD:408798	19/74 (26%)
		TSP1_spondin	1404..1463	CDD:408798	10/58 (17%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3538
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900