DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nolo and Papln

DIOPT Version :9

Sequence 1:	NP_001036374.1	Gene:	nolo / 35424	FlyBaseID:	FBgn0051619	Length:	1394	Species:	Drosophila melanogaster
Sequence 2:	NP_001192272.1	Gene:	Papln / 170721	MGIID:	2386139	Length:	1302	Species:	Mus musculus

Alignment Length:	1585	Identity:	317/1585 - (20%)
Similarity:	461/1585 - (29%)	Gaps:	646/1585 - (40%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 KIWAALFVTTLCLLDTQAAWAKKLNASQAFDDTWNTASDLENELEQQKRSKVQSAGGGGGAGGGP 75
            :::..||  :|.|..|..:||:.:...   .|||.|
Mouse     2 QLFPLLF--SLLLTSTPGSWARNVRRQ---SDTWGT----------------------------- 32

  Fly    76 GQWSSWSDWSTCSRTCDGGIMHQMRRCGSP-----GSCRGESTRYRICNMQPCPE-QQDFRSSQC 134
                 |.:||.|||||.|||..:.|.|.|.     .||.|.:..:|.|:.:.||: .:|||:.||
Mouse    33 -----WGEWSPCSRTCGGGISFRERPCYSQRRDGGTSCVGPARSHRTCHTESCPDGVRDFRAEQC 92

  Fly   135 SAYNDVPYDGTLYKWTPHYDYVEPCALTC--RGHPAHLVEDISRETGDGNAEEAEHYDEQSVIVQ 197
            :.::...:.|..|:|.|:|.....|.|.|  :|                   :..:|..:..:|
Mouse    93 AEFDGTDFQGRRYRWLPYYAAPNKCELNCIPKG-------------------QNFYYKHKDAVV- 137

  Fly   198 LSARVQDGTRCRSGSLDMCIQGKCQRVGCDLKIGSTKKIDGCGVCGGDGNSC------------- 249
                  |||.|..|..|:|:.|.|:.||||.|:.|.|:.|.|..|||||:||
Mouse   138 ------DGTPCEPGQRDICVDGVCRVVGCDHKLDSIKQEDKCLQCGGDGSSCYPVTGTFDGNDLS 196

  Fly   250 ----------------------------------------------------------------- 249

Mouse   197 RGYNQIFIIPAGATSIRIEEAAASRNFLAVKSIRGEYYLNGHWTIEAAQALPVASTVLQYERGVE 261

  Fly   250 --------------SQPL---------------------------FNWEMAPMSQCSVTCGSGYK 273
                          |:||                           |:|.......||..||.|::
Mouse   262 GDLAPERLQARGPTSEPLVIELLSQESNPGVHYEYYLPANDPGRGFSWSHGSWGDCSAECGGGHQ 326

  Fly   274 MSRPICRNRLTNADVDDTLCSVTNRPEASVEQCNTHSCPP------------------------R 314
            .....|  .:.|....|.:|....|| .....|||..||.                        .
Mouse   327 SRLVFC--TIDNEAYPDHMCQHQPRP-THRRSCNTQPCPKTKRISFLHRPGAWRYAGVQHMCGNS 388

  Fly   315 WIADDWSTCSRLCGHGYRERMVVC-AEESNGIKTRVADIMCR--TPKPPTQETCIIEECPHWEVE 376
            |....|:.||..||.|.:.|.|.| :.:..|.:....:..|.  ..||||.:.|.::.|..|.||
Mouse   389 WKVGPWTPCSVSCGGGVQSRSVYCISSDGTGGQEAAEETQCAGLVGKPPTTQACNLQHCAVWSVE 453

  Fly   377 DWTGCSVSCGQGIQMRGVECKSTDGSLSAKCDPLTKPGSMQQCSTGIHCGGSLNKVGGTIIVGSS 441
            .|..|||:||.||:.|.|.|:..:||                   .:|....|.|
Mouse   454 PWGECSVTCGTGIRKRSVTCRGDEGS-------------------PVHAAACLLK---------- 489

  Fly   442 RSLNERSERQLDSSDADEDNEDENDEGDDVDDLESGQDTDDGEGLSYADQPLLYAHRTQSRLNQE 506
                                                            |||.|    |:..:.:.
Mouse   490 ------------------------------------------------DQPTL----TEPCVQEA 502

  Fly   507 APDEPRTMHLMNGNSNNNFNRGEDESEGPSLDPTYIKDNEWSPCSVTCGEGIRRRTYNCKIFLEY 571
            .|                ..||:....|           .||.||.:||.|||||
Mouse   503 CP----------------VFRGQAWHVG-----------SWSLCSKSCGSGIRRR---------- 530

  Fly   572 SRTVATVN-DSLC---EGKKPHDEVERCVEDPCMLPSHGFDDQFPR---------DSIKVGVSEP 623
             :.|.|:. ...|   :..|| .|:|.|...||.||......|.||         ...:|.....
Mouse   531 -QVVCTIGPPGRCVDLQSSKP-AEMEACNRQPCHLPQEVPSIQDPRTRSSDPRMLSGPRVSPVSD 593

  Fly   624 GKTYVWREQGYTSCSASCLGGVEELIINCVREDNGRVVSPFLCSPETKPEAR----VRTCNDRP- 683
            |:...|.........::...|.:..:.:..|       :|.|..|..:|..|    .|.|...|
Mouse   594 GREQQWAPLERPRAQSNPREGQDPNLSSAGR-------APTLQRPPHQPPLRPSSGPRDCRHSPH 651

  Fly   684 --CP--------PRWN----------YSDYTPCSKSCGIGIKTREVQCIHEVTRGGDNTMVVPNS 728
              ||        |:|.          .|.|..|..........::..|..  :.|.|||...|.|
Mouse   652 GCCPDGHTPSLGPQWQGCPLAGASCLQSRYGCCPDGVSAAEGPQQAGCTR--SHGSDNTGNRPGS 714

  Fly   729 MC----------PQPPPADRQYC----------NVLDC----------------PVR-------W 750
            ..          ||....:...|          ||...                |||       .
Mouse   715 RAVASKNPKIHQPQAHEGEPSECRSSRFGCCYDNVASAAGPLGEGCVGQPSYAYPVRCLLPSAQG 779

  Fly   751 EVGEWS----------KCS-------HTCGYGFKDRKVECKQIMAQEHKIERPES---------- 788
            ..|:|:          :|:       |.....|...: ||......:|...|||:
Mouse   780 SCGDWAARWYFVASVGRCNRFWYGGCHGNANNFASEQ-ECMNTCRGQHGPRRPEAGAAGHRAHVD 843

  Fly   789 ------------------------MCPSAKPADKKPCNVKPCPPEDP--------------KPVI 815
                                    ..||..|..::.   :|.|.|.|              :|.:
Mouse   844 GGQRGPGGQQEPDWHRAGATIPRLPSPSGSPWRREQ---EPAPGEPPHIPAYGNRPGGQEIRPRV 905

  Fly   816 -----QINNSTHIQHDPKKTKITLKVGGAGLVFF--GTQVKIKCPVKRYNRTKIKWSKDHKPLQR 873
                 :...:....|.| ..:|.|......||..  |..|::.||....:..:..|.|:.:|:.
Mouse   906 PGLDREARPAVPPTHSP-SYRIRLAGSEPSLVQAAPGQAVQLFCPGNIPSEFQAGWQKEGRPIS- 968

  Fly   874 SRKYKVSKKGALRILDITFRDAGVYSCHAGLSSAEISIEVKAKPGQQIEELEQQETERLVREKSG 938
            |.:|::...|:|.|..:...|||:|||.:            .:||.:.:|::.:
Mouse   969 SNRYQLQADGSLIISRLRPEDAGIYSCGS------------HRPGHEPQEIQLR----------- 1010

  Fly   939 TEALTSADM--------------KSAD------GTGTSTAVGPNSHVNGSAQQHGSRRRQQQSER 983
               :|..||              ::.|      ..||....|.:..::.|..:..:|.|   .:|
Mouse  1011 ---VTGGDMAVFPEGQPRHFPEPRNPDLGHGPPHRGTGAEAGGHRVLSPSHPRPATRLR---LDR 1069

  Fly   984 LQNG------RERSRRP-KSDGVQHADSSIMEDDLSQLVQSEDRIPQP--------ASASSGGSR 1033
            .|.|      .:|.|.. :::|..........|  .|||.|.....||        .....||
Mouse  1070 TQPGVVDASPGQRIRLTCRAEGFPVPTIEWQRD--GQLVSSPRHQVQPDGSLVISRVDVEDGG-- 1130

  Fly  1034 TRTLLAMPYFQALLSNLQLLWPLQRFKDSRGQHLLQGEALKFGIDLSGKDFDQDESERVPAEQLP 1098
                    |:..:..|.|          .|.|..:|...|: .:.::|               ||
Mouse  1131 --------YYSCVAFNGQ----------DRDQRWVQLRVLR-ELTITG---------------LP 1161

  Fly  1099 PEVLHSPSDPEYHEPSKSGPEADVPHVPPHMPHARWETGA---AASTTAVPSLPNDGFVYRWMLG 1160
            |.|..:..|........:|...::          ||....   .|....|...| ||.:....|.
Mouse  1162 PAVTVAEGDTARLLCVVAGESVNI----------RWSRNGLPIQADGHRVHQSP-DGTLLIHNLR 1215

  Fly  1161 EWSQCSQECGA-AGSGLQRRTVSCQRAAKATSSGSTSDTENNVVDNSECFAHGLELPEFFQSCGN 1224
            ...:.|..|.| .||....|:...:.|..|.::.| .|...:.:|..|.....|.|..  |.|||
Mouse  1216 PRDEGSYTCSAFRGSQAVSRSTEVKVALPAPAAQS-RDLGKDCIDQPELANCALILQA--QLCGN 1277

  Fly  1225 E----------------ACPQWSKG 1233
            |                |.|.|.:|
Mouse  1278 EYYSSFCCASCSRFQPNAQPVWQQG 1302

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nolo	NP_001036374.1	TSP1	78..124	CDD:214559	19/50 (38%)
		TSP1	546..600	CDD:214559	19/57 (33%)
		TSP1	750..807	CDD:214559	15/107 (14%)
		IGc2	845..902	CDD:197706	18/56 (32%)
		PLAC	1355..1384	CDD:285849
Papln	NP_001192272.1	TSP1	30..81	CDD:214559	21/84 (25%)
		ADAM_spacer1	184..299	CDD:368694	3/114 (3%)
		TSP1	309..362	CDD:214559	15/55 (27%)
		TSP1	389..447	CDD:214559	17/57 (30%)
		TSP1	447..504	CDD:214559	25/137 (18%)
		TSP1	511..562	CDD:214559	21/73 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	563..648		17/91 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	694..737		9/44 (20%)
		Kunitz_BPTI	771..822	CDD:333766	8/51 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	822..924		14/105 (13%)
		Papilin_u7	831..922	CDD:374683	11/93 (12%)
		Ig	932..>995	CDD:386229	18/63 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1024..1064		5/39 (13%)
		I-set	1070..1141	CDD:369462	17/92 (18%)
		Ig	1157..1241	CDD:386229	21/109 (19%)
		PLAC	1257..1289	CDD:370061	9/33 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3538
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810