DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eEF2 and EFT1

DIOPT Version :9

Sequence 1:	NP_525105.2	Gene:	eEF2 / 35422	FlyBaseID:	FBgn0000559	Length:	844	Species:	Drosophila melanogaster
Sequence 2:	NP_014776.1	Gene:	EFT1 / 854301	SGDID:	S000005659	Length:	842	Species:	Saccharomyces cerevisiae

Alignment Length:	848	Identity:	568/848 - (66%)
Similarity:	685/848 - (80%)	Gaps:	10/848 - (1%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MVNFTVDEIRGLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQE 65
            ||.||||::|.||||..|:|||||||||||||||||||||.:||||:.|||||.|||||||||||
Yeast     1 MVAFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFTDTRKDEQE 65

  Fly    66 RCITIKSTAISMYFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 130
            |.|||||||||:|.|:.::|:..|    :::.:...|||||||||||||||||||||||||||||
Yeast    66 RGITIKSTAISLYSEMSDEDVKEI----KQKTDGNSFLINLIDSPGHVDFSSEVTAALRVTDGAL 126

  Fly   131 VVVDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLDAEELYQTFQRIVENVNVIIAT 195
            ||||.:.|||||||||||||:.|||||::.:||:||||||||:..|:|||||.|.||:||||::|
Yeast   127 VVVDTIEGVCVQTETVLRQALGERIKPVVVINKVDRALLELQVSKEDLYQTFARTVESVNVIVST 191

  Fly   196 YNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKT 260
            |.|:  .:|:|:|.|::|:|.|||||||||||::||:..|::||.:|..|:|:||||::|||.||
Yeast   192 YADE--VLGDVQVYPARGTVAFGSGLHGWAFTIRQFATRYAKKFGVDKAKMMDRLWGDSFFNPKT 254

  Fly   261 KKW-QKQKEADNK---RSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVTLKHEDKDKDGKAL 321
            ||| .|..:|:.|   |:|.|:|||||:::|.||||:||:||..||||:.:.||.::||.:||||
Yeast   255 KKWTNKDTDAEGKPLERAFNMFILDPIFRLFTAIMNFKKDEIPVLLEKLEIVLKGDEKDLEGKAL 319

  Fly   322 LKTVMRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIAVKSCDPDGPLMMYISK 386
            ||.|||.:|||.:|||:||.:||||||.||.||.|.|||||.||...||:|:|||...||:|:||
Yeast   320 LKVVMRKFLPAADALLEMIVLHLPSPVTAQAYRAEQLYEGPADDANCIAIKNCDPKADLMLYVSK 384

  Fly   387 MVPTSDKGRFYAFGRVFAGKVATGQKCRIMGPNYTPGKKEDLYEKAIQRTILMMGRYVEAIEDVP 451
            |||||||||||||||||||.|.:|||.||.||||.||||:||:.|||||.:|||||:||.|:|.|
Yeast   385 MVPTSDKGRFYAFGRVFAGTVKSGQKVRIQGPNYVPGKKDDLFIKAIQRVVLMMGRFVEPIDDCP 449

  Fly   452 SGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKS 516
            :|||.||||:||||:||||:||.:.|||||||||||||||:||||.||..||||||||||||:||
Yeast   450 AGNIIGLVGIDQFLLKTGTLTTSETAHNMKVMKFSVSPVVQVAVEVKNANDLPKLVEGLKRLSKS 514

  Fly   517 DPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSEESDQMCLSKS 581
            ||.|...:.||||||:||.|||||||||:|||.|||.:|||.|.|||:|||||..||.|..||||
Yeast   515 DPCVLTYMSESGEHIVAGTGELHLEICLQDLEHDHAGVPLKISPPVVAYRETVESESSQTALSKS 579

  Fly   582 PNKHNRLLMKALPMPDGLPEDIDNGDVSAKDEFKARARYLSEKYDYDVTEARKIWCFGPDGTGPN 646
            ||||||:.:||.|:.:.:...|:||.::.:|:||||||.:::.|.:|||:|||||||||||.|||
Yeast   580 PNKHNRIYLKAEPIDEEVSLAIENGIINPRDDFKARARIMADDYGWDVTDARKIWCFGPDGNGPN 644

  Fly   647 FILDCTKSVQYLNEIKDSVVAGFQWASKEGILADENLRGVRFNIYDVTLHADAIHRGGGQIIPTT 711
            .::|.||:||||:||||||||.||||:|||.:..|.:|.||.||.|||||||||||||||||||.
Yeast   645 LVIDQTKAVQYLHEIKDSVVAAFQWATKEGPIFGEEMRSVRVNILDVTLHADAIHRGGGQIIPTM 709

  Fly   712 RRCLYAAAITAKPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEENQVVGTPMFVVKAYLPV 776
            ||..||..:.|.|::.|||:|.||||||.||||||.|||::||.|..|.|..|||:|.|||||||
Yeast   710 RRATYAGFLLADPKIQEPVFLVEIQCPEQAVGGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPV 774

  Fly   777 NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQYL 841
            |||||||.:||..||||||||.|||||..|..||.:|:||...||...|||.|:||.:|...:|.
Yeast   775 NESFGFTGELRQATGGQAFPQMVFDHWSTLGSDPLDPTSKAGEIVLAARKRHGMKEEVPGWQEYY 839

  Fly   842 DKL 844
            |||
Yeast   840 DKL 842

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eEF2	NP_525105.2	PTZ00416	1..844	CDD:240409	566/846 (67%)
		EF2	20..236	CDD:206672	151/215 (70%)
		EF2_snRNP_like_II	380..474	CDD:293901	71/93 (76%)
		EF2_snRNP_III	489..560	CDD:293918	52/70 (74%)
		aeEF2_snRNP_like_IV	560..732	CDD:238839	108/171 (63%)
		eEF2_snRNP_like_C	728..807	CDD:239763	57/78 (73%)
EFT1	NP_014776.1	PTZ00416	1..842	CDD:240409	566/846 (67%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157342080
Domainoid	1	1.000	430	1.000	Domainoid score	I68
eggNOG	1	0.900	-	-		E1_COG0480
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1116	1.000	Inparanoid score	I18
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG62214
OrthoFinder	1	1.000	-	-		FOG0002412
OrthoInspector	1	1.000	-	-		oto100201
orthoMCL	1	0.900	-	-		OOG6_100212
Panther	1	1.100	-	-	LDO	PTHR42908
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R367
SonicParanoid	1	1.000	-	-		X1605
TreeFam	1	0.960	-	-
	14	13.790