DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eEF2 and gfm1

DIOPT Version :9

Sequence 1:	NP_525105.2	Gene:	eEF2 / 35422	FlyBaseID:	FBgn0000559	Length:	844	Species:	Drosophila melanogaster
Sequence 2:	NP_001073463.1	Gene:	gfm1 / 561840	ZFINID:	ZDB-GENE-061013-79	Length:	745	Species:	Danio rerio

Alignment Length:	826	Identity:	208/826 - (25%)
Similarity:	343/826 - (41%)	Gaps:	198/826 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 GLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIA-----GAKAGETRFTDTRKDEQERCITI 70
            |:...:| |||:.:.||:|.||:|||:.::...|.||     ..|.|.....|:.:.|::|.|||
Zfish    34 GITPNER-IRNIGISAHIDSGKTTLTERVLYYTGRIAEMHEVRGKDGVGAIMDSMELERQRGITI 97

  Fly    71 KSTA-ISMYFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVD 134
            :|.| .:|:                     |...||:||:||||||:.||..:|||.|||::|:.
Zfish    98 QSAATYTMW---------------------KNHNINIIDTPGHVDFTIEVERSLRVLDGAVLVLC 141

  Fly   135 CVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLDAEELYQTFQRIVENVNVIIATYNDD 199
            .|.||..||.||.||.....:..:.|:||:||.........::|.....:....||:
Zfish   142 AVGGVQCQTVTVNRQMKRYSVPFLTFINKLDRLGANPNRALQQLRTKLNQNAAFVNI-------- 198

  Fly   200 GGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKTKKWQ 264
                          .:|....|.|....:::.|.::...|            ||:..........
Zfish   199 --------------PIGLEGNLRGIIDLIEERSIVFDGPF------------GESVRYEDIPPEM 237

  Fly   265 KQKEADNKRSFCMYILDPIYKVFDAIMNYKKEEIGTLL--EKIGVTLKHEDKDKDGKALLK--TV 325
            :.:.||.::           ::.:.:.| ..|.:|.:.  |::...|       |.||.::  ||
Zfish   238 RSEAADRRQ-----------ELVECVAN-ADETLGEMFLEERVPTVL-------DLKAAVRRATV 283

  Fly   326 MRTWLP--AGEA--------LLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIAVKSCDPDGPL 380
            .|::.|  .|.|        ||..:..:||:|...|.|.: :..||..:....:...:.|...|.
Zfish   284 KRSFSPVLVGSALKNKGVQPLLDAVLEYLPNPTEVQNYAI-LNEEGESEGSKILMDSTRDDTQPF 347

  Fly   381 MMYISKMVPTSDKGRF--YAFGRVFAG---------KVATGQKCRIMGPNYTPGKKEDLYEKAIQ 434
            :....|:    :.|||  ..:.||:.|         ...||::.|                  :|
Zfish   348 VGLAFKL----EAGRFGQLTYVRVYQGCLRKTDYIHNSRTGRRVR------------------VQ 390

  Fly   435 RTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSV-SPVVRVAVEPK 498
            |.:.:....:|.:|...:|:||.|.|:|   ..:|...|.::..|:.:....| .||:.:|:.|.
Zfish   391 RLVRLHADQMEDVEVAYAGDICALFGID---CASGDTFTARNNANLSMESIHVPEPVISMAIRPS 452

  Fly   499 NPADLPKLVEGLKRLAKSDPMVQCIIE-ESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPV 562
            |..|..||.:|:.|..:.||..:...: ||.|.||:|.|||||||..:.:|.:::| |.....|.
Zfish   453 NKNDTDKLSKGISRFTREDPTFRVHFDTESKETIISGMGELHLEIYSQRMEREYSC-PCVMGKPK 516

  Fly   563 VSYRETVSE----ESDQMCLSKSPNKHNRLLMKALPMPDGLPEDIDNGDVSAKDEFKARARYLSE 623
            |::|||::.    |......|....::.:::        |:.|.:|                 ||
Zfish   517 VAFRETLTSAVPFEYTHKKQSGGSGQYGKVI--------GVLEPLD-----------------SE 556

  Fly   624 KYDYDVTEARKIWCFGPDGTGPNFILDCTKSVQYLNEIKDSVVAGFQWASKEGILADENLRGVRF 688
            .|    |:..    |..:..|.|.          ..:...:|..||:.|.::|.|....:.||||
Zfish   557 NY----TKVE----FSDETVGTNI----------PKQFVPAVEKGFRDACEKGPLIGHKISGVRF 603

  Fly   689 NIYDVTLH-ADA-----IHRGGGQIIPTTRRCLYAAAITAKPRLMEPVYLCEIQCPEVAVGGIYG 747
            .:.|...| .|:     |..|.|.:    ::.|..|.:.    ::|||...||..|....|.:..
Zfish   604 VLEDGAHHMVDSNEISFIRAGEGAV----KQALEKATVV----ILEPVMSVEIVAPNEFQGAVIA 660

  Fly   748 VLNRRRGHVFEENQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQ 793
            .:|||.|.:..::...|  .|.:.|.:|:|:.||:..:|||.|.|:
Zfish   661 GVNRRHGVISGQDGADG--YFTLYADIPLNDMFGYATELRSCTEGK 704

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eEF2	NP_525105.2	PTZ00416	1..844	CDD:240409	208/826 (25%)
		EF2	20..236	CDD:206672	65/221 (29%)
		EF2_snRNP_like_II	380..474	CDD:293901	21/104 (20%)
		EF2_snRNP_III	489..560	CDD:293918	28/71 (39%)
		aeEF2_snRNP_like_IV	560..732	CDD:238839	38/181 (21%)
		eEF2_snRNP_like_C	728..807	CDD:239763	23/66 (35%)
gfm1	NP_001073463.1	EF-G	43..315	CDD:206673	87/345 (25%)
		PRK12740	47..726	CDD:237186	203/812 (25%)
		mtEFG1_II_like	347..427	CDD:293908	21/104 (20%)
		EFG_III	441..516	CDD:293919	28/75 (37%)
		EFG_mtEFG1_IV	519..635	CDD:238715	32/162 (20%)
		mtEFG1_C	641..718	CDD:239764	23/66 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0480
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810