DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment eEF2 and eftud2

DIOPT Version :9

Sequence 1:	NP_525105.2	Gene:	eEF2 / 35422	FlyBaseID:	FBgn0000559	Length:	844	Species:	Drosophila melanogaster
Sequence 2:	NP_956802.2	Gene:	eftud2 / 393480	ZFINID:	ZDB-GENE-040426-1569	Length:	973	Species:	Danio rerio

Alignment Length:	861	Identity:	326/861 - (37%)
Similarity:	513/861 - (59%)	Gaps:	42/861 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 FTVDEIRGLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKA-GIIAGAKAGETRFTDTRKDEQERC 67
            :.::.:..|||....|||:::..|:.|||:...|.|:.:. ..|......:.|:||....||||.
Zfish   115 YDMEFLADLMDSSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRDDEDLRYTDILFTEQERG 179

  Fly    68 ITIKSTAISMYFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGALVV 132
            :.||||.::|..            ||.|.   |.:|.|::|:||||:||.|||:|:|::||.::.
Zfish   180 VGIKSTPVTMVL------------PDSRG---KSYLFNIMDTPGHVNFSDEVTSAVRLSDGIVLF 229

  Fly   133 VDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLDAEELYQTFQRIVENVNVIIATYN 197
            :|...||.:.||.:::.|:.||:...:.:||:||.::||:|...:.|...:.||:.||.:::||:
Zfish   230 IDAAEGVMLNTERLIKHAVQERLAITICINKIDRLIVELKLPPTDAYYKLRHIVDEVNGLLSTYS 294

  Fly   198 DDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKF-KIDVVKLMNRLWGENFFNAKTK 261
            .|    ..:.|.|..|:|.|.|..:...|||..|:::||:.: .|..::...||||:.:||.||:
Zfish   295 TD----ESLIVSPLLGNVCFASSQYCICFTLGSFAKIYSDTYGDISYMEFAKRLWGDIYFNPKTR 355

  Fly   262 KW-QKQKEADNKRSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVTLKHEDKDKDGKALLKTV 325
            |: :|...::::|||..::|:|:||:...::......:..:|:::|:.|..|:...:.|.||:.|
Zfish   356 KFTKKAPNSNSQRSFVEFVLEPLYKILSQVVGDVDTSLPRVLDELGIHLTKEELKLNIKPLLRLV 420

  Fly   326 MRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIAVKSCDPDGPLMMYISKMVPT 390
            ...:......|:.|...|:|||....:.::|..|.|..|.:....:..||||||||.:.:||..|
Zfish   421 CNRFFGEFTGLVDMCVQHIPSPQGGARAKIEHTYTGGLDSDLGETMSECDPDGPLMCHTTKMYST 485

  Fly   391 SDKGRFYAFGRVFAGKVATGQKCRIMGPNYTPGKKEDLYEKAIQRTILMMGRYVEAIEDVPSGNI 455
            .|..:|:|||||.:|.:..||..:::|.||:...:||.....:.|..:.:.||...:..||:||.
Zfish   486 DDGVQFHAFGRVLSGTLQAGQPVKVLGENYSLEDEEDSQICTVGRLWISVARYQIEVNRVPAGNW 550

  Fly   456 CGLVGVDQFLVKTGTITT---FKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSD 517
            ..:.|.||.:|||.|||.   .::|...:.:||:.:.|:::||||.||::|||:::||:::.||.
Zfish   551 VLIEGCDQPIVKTATITEPRGNEEAQIFRPLKFNTASVIKIAVEPVNPSELPKMLDGLRKVNKSY 615

  Fly   518 PMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSEESDQMCLSKSP 582
            |.:...:||||||:|.|.|||:|:..:.||.:.::.|.:|.:||||::.|||.|.|...|.:::|
Zfish   616 PSLTTKVEESGEHVILGIGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETP 680

  Fly   583 NKHNRLLMKALPMPDGLPEDIDNGDVSAKDEFKARARYLSEKYDYDVTEARKIWCFGPDGTGPNF 647
            ||.|::.|.|.|:..||.|||:|..|......|....:...|||:|:..||.||.||||.||||.
Zfish   681 NKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDTTGPNI 745

  Fly   648 ILDCTKSVQ----YLNEIKDSVVAGFQWASKEGILADENLRGVRFNIYDVTLHADAIHRGGGQII 708
            ::|.|...:    .|..:|||:|.||||.::||.|.||.:|.|:|.|.|..:..:.:||||||:|
Zfish   746 LVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDEPIRNVKFKILDAVIAQEPLHRGGGQVI 810

  Fly   709 PTTRRCLYAAAITAKPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEENQVVGTPMFVVKAY 773
            ||.||.:|:|.:.|.||||||.|..|:|.|...|..:|.||.||||||.::..:.|:|::.:||:
Zfish   811 PTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAF 875

  Fly   774 LPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGD---------PSEPSSKPY---AIVQDTRK 826
            :|..:||||..|||::|.||||...||.|||::|||         |.||...|:   ..:..||:
Zfish   876 IPAIDSFGFETDLRTHTQGQAFALSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRR 940

  Fly   827 RKGLKEGLPDLSQYLD 842
            ||||.|.: .:|::.|
Zfish   941 RKGLSEDV-SISKFFD 955

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eEF2	NP_525105.2	PTZ00416	1..844	CDD:240409	326/861 (38%)
		EF2	20..236	CDD:206672	74/216 (34%)
		EF2_snRNP_like_II	380..474	CDD:293901	36/96 (38%)
		EF2_snRNP_III	489..560	CDD:293918	31/70 (44%)
		aeEF2_snRNP_like_IV	560..732	CDD:238839	82/175 (47%)
		eEF2_snRNP_like_C	728..807	CDD:239763	38/78 (49%)
eftud2	NP_956802.2	EFTUD2	4..111	CDD:292623
		PTZ00416	123..955	CDD:240409	325/851 (38%)
		Snu114p	131..329	CDD:206730	74/216 (34%)
		EF2_II_snRNP	475..568	CDD:293907	35/92 (38%)
		snRNP_III	587..658	CDD:293921	31/70 (44%)
		EF2_IV_snRNP	658..835	CDD:238840	83/176 (47%)
		eEF2_C_snRNP	830..909	CDD:239765	38/78 (49%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0480
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D140796at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100212
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.720