DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eEF2 and Gfm1

DIOPT Version :9

Sequence 1:	NP_525105.2	Gene:	eEF2 / 35422	FlyBaseID:	FBgn0000559	Length:	844	Species:	Drosophila melanogaster
Sequence 2:	NP_613057.2	Gene:	Gfm1 / 28030	MGIID:	107339	Length:	751	Species:	Mus musculus

Alignment Length:	841	Identity:	215/841 - (25%)
Similarity:	346/841 - (41%)	Gaps:	209/841 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 IRNMSVIAHVDHGKSTLTDSLVSKAGIIA-----GAKAGETRFTDTRKDEQERCITIKSTA-ISM 77
            |||:.:.||:|.||:|||:.::...|.||     ..|.|.....|:.:.|::|.|||:|.| .:|
Mouse    47 IRNIGISAHIDSGKTTLTERVLYYTGRIATMHEVKGKDGVGAVMDSMELERQRGITIQSAATYTM 111

  Fly    78 YFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQ 142
            :.::.                     ||:||:||||||:.||..||||.|||::|:..|.||..|
Mouse   112 WKDIN---------------------INIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQ 155

  Fly   143 TETVLRQAIAERIKPILFMNKMDR--------------------ALLELQLDAEELYQTFQRIVE 187
            |.||.||.....:..:.|:||:||                    |.:::.:..|   ..|:.|::
Mouse   156 TMTVSRQMKRYNVPFLTFINKLDRMGSNPSRALQQMRSKLNHNAAFVQIPIGLE---GDFKGIID 217

  Fly   188 NVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWG 252
            .:......::.|.|.:  ||.|.      ..:||...|...:|  |:      |:.|...:...|
Mouse   218 LIEERAIYFDGDFGQI--VRYDE------IPAGLRAAAADHRQ--EL------IECVANSDEQLG 266

  Fly   253 ENFFNAKTKKWQKQKEADNK----RSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVTLKHED 313
            |.|...|.......|.|..:    |||     .|::             :|:.|:..||
Mouse   267 ELFLEEKIPSVSDLKRAIRRATLSRSF-----TPVF-------------LGSALKNKGV------ 307

  Fly   314 KDKDGKALLKTVMRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIAVK-SCDPD 377
                                :.||..:..:||:|...|.|  .:|.:....::..|.:. ..|..
Mouse   308 --------------------QPLLDAVLEYLPNPSEVQNY--AILNQNDSKEKTKILMNPQRDDS 350

  Fly   378 GPLMMYISKMVPTSDKGRF--YAFGRVFAGKVATGQKCRIMGPNYTPGKKEDLYEKAIQRTILMM 440
            .|.:....|:    :.|||  ..:.|.:.|::..|....    |...|||     ..:||.:.|.
Mouse   351 HPFVGLAFKL----EAGRFGQLTYVRNYQGELKKGSTIY----NTRTGKK-----VRVQRLVRMH 402

  Fly   441 GRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSV-SPVVRVAVEPKNPADLP 504
            ...:|.:|:|.:|:||.|.|:|   ..:|...|.||..::.:....| .||:.:|:.|.|..||.
Mouse   403 ADMMEDVEEVYAGDICALFGID---CASGDTFTNKDNSDLSMESIHVPEPVISIAMRPSNKNDLE 464

  Fly   505 KLVEGLKRLAKSDPMVQCIIE-ESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRET 568
            |..:|:.|..:.||..:...: ||.|.|::|.|||||||..:.:|.::.| |.....|.|::|||
Mouse   465 KFSKGIGRFTREDPTFKVHFDPESKETIVSGMGELHLEIYAQRMEREYGC-PCITGKPKVAFRET 528

  Fly   569 ----VSEESDQMCLSKSPNKHNRLLMKALPMPDGLPEDIDNGDVSAKDEFKARARYLSEKYDYDV 629
                |..:......|....:..:::....|:|   |||.      .|.||.      .|.:..:|
Mouse   529 IVAPVPFDFTHKKQSGGAGQFGKVIGVLEPLP---PEDY------TKLEFS------DETFGSNV 578

  Fly   630 TEARKIWCFGPDGTGPNFILDCTKSVQYLNEIKDSVVAGFQWASKEGILADENLRGVRFNIYDVT 694
            .:.     |.|                       :|..||..|.::|.|:...|.|:||.:.|..
Mouse   579 PKQ-----FVP-----------------------AVEKGFLDACEKGPLSGHKLSGLRFVLQDGA 615

  Fly   695 LH-ADA-----IHRGGG---QIIPTTRRCLYAAAITAKPRLMEPVYLCEIQCPEVAVGGIYGVLN 750
            .| .|:     |..|.|   |.:.....|           ::||:...|:..|....|.::..:|
Mouse   616 HHMVDSNEISFIRAGEGALKQALANGTLC-----------IIEPIMSVEVIAPNEFQGTVFAGIN 669

  Fly   751 RRRGHVFEENQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPS 811
            ||.|.:..::.:  ...|.:.|.:|:|..||::.:|||.|.|:.  :...::.:..|..||
Mouse   670 RRHGVITGQDGI--EDYFTLYADVPLNNMFGYSTELRSCTEGKG--EYTMEYCRYQPCSPS 726

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eEF2	NP_525105.2	PTZ00416	1..844	CDD:240409	215/841 (26%)
		EF2	20..236	CDD:206672	72/241 (30%)
		EF2_snRNP_like_II	380..474	CDD:293901	24/95 (25%)
		EF2_snRNP_III	489..560	CDD:293918	27/71 (38%)
		aeEF2_snRNP_like_IV	560..732	CDD:238839	39/184 (21%)
		eEF2_snRNP_like_C	728..807	CDD:239763	20/78 (26%)
Gfm1	NP_613057.2	EF-G	49..321	CDD:206673	91/355 (26%)
		PRK12740	53..732	CDD:237186	212/835 (25%)
		mtEFG1_II_like	353..432	CDD:293908	24/94 (26%)
		EFG_III	447..522	CDD:293919	27/75 (36%)
		EFG_mtEFG1_IV	525..641	CDD:238715	34/158 (22%)
		mtEFG1_C	647..724	CDD:239764	21/80 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0480
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810