DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eEF2 and Efl1

DIOPT Version :9

Sequence 1:	NP_525105.2	Gene:	eEF2 / 35422	FlyBaseID:	FBgn0000559	Length:	844	Species:	Drosophila melanogaster
Sequence 2:	NP_001153144.1	Gene:	Efl1 / 101592	MGIID:	2141969	Length:	1127	Species:	Mus musculus

Alignment Length:	1130	Identity:	330/1130 - (29%)
Similarity:	514/1130 - (45%)	Gaps:	323/1130 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MVNFTVDEIRGLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQE 65
            ||...||::..|.....||||:.|:|||||||:||.|.|:|..|||:...||:.|:.|:|:|||.
Mouse     1 MVLSGVDKMIRLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQV 65

  Fly    66 RCITIKSTAISMYFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 130
            |.||:||:|||:::....::                :|||||||||||||||||:.|:|:.||.:
Mouse    66 RGITMKSSAISLHYAEGHEE----------------YLINLIDSPGHVDFSSEVSTAVRICDGCI 114

  Fly   131 VVVDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLDAEELYQTFQRIVENVNVIIAT 195
            :|||.|.|||.||:.|||||..|.|:|:|.:||:||.::||:...:|.|...:.|:|.:|.:..|
Mouse   115 IVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGT 179

  Fly   196 ---------------------YNDDGGPM------------GEVRVDPSKGSVGFGSGLHGWAFT 227
                                 :::.|..:            .::...|.:|:|.|.|.:.||.|.
Mouse   180 LFTSKVLEERAERETESQAKPHSEQGEQVYDWSAGLEDVDDSQLYFSPEQGNVVFTSAIDGWGFG 244

  Fly   228 LKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKTKKWQKQKEADNKRS-FCMYILDPIYKVFDAIM 291
            ::.|:.:||:|..|....|:..|||:.:.|.|.||..|..:|..|:. |...||:.|:.::||::
Mouse   245 IEHFARIYSQKIGIKKEVLLKTLWGDYYINMKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVL 309

  Fly   292 NYKKEEIGTLLEKIGVTL-KHEDKDKDGKALLKTVMRTWLPAGEALLQMIAIHLPSPVVAQKYRM 355
            ...||:|..::..:|:.: ..|.:..|.|..:..:...|||...|:|.|:...||||:.....|:
Mouse   310 KKDKEKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCHKLPSPLDMTSERV 374

  Fly   356 EML-------YEG--PHDDEAAIAVKSC--DPDGPLMMYISKMV--------------------- 388
            |.|       :|.  |.......|...|  :...|:::::|||.
Mouse   375 EKLLCTGSQTFESLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDVKALPQNKPRPLTQEEMA 439

  Fly   389 ------------------------PTSDKG----------------------------------R 395
                                    ||.|.|                                  .
Mouse   440 QRRERARQRHAEKLAAAQGQTSQGPTQDGGALETSPHEDEPRGDEPDVASVSRQPVSQEESSQEA 504

  Fly   396 FYAFGRVFAGKVATGQKCRIMGPNYTP------------GKKEDLYEK------AIQRTILMMGR 442
            |.||.|||:|....|:|..::||.|:|            ...|||...      .::...|:|||
Mouse   505 FIAFARVFSGIARRGKKIFVLGPKYSPVDFLQRVPLGFSAPLEDLPPVPHMACCTLENLYLLMGR 569

  Fly   443 YVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLV 507
            .:|.:|:||.||:.|:.|:..|::|:.|:.:.........:.|..:|:||||||||:|:::|:||
Mouse   570 ELEDLEEVPPGNVLGIGGLQDFVLKSATLCSLPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLV 634

  Fly   508 EGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSE- 571
            :|:|.|.::||.||.:|:|:|||::..|||:||:.||.||.|..|.|.:..|:|::.:|||:::
Mouse   635 KGMKLLNQADPCVQVLIQETGEHVLVTAGEVHLQRCLDDLRERFAKIHISVSEPIIPFRETITKP 699

  Fly   572 -----------------------------------ESDQMCLSKSPNKHNRLLMKALPMPDGLPE 601
                                               :||.:....:|||...|.::|:|:|:.:..
Mouse   700 PKVDMVNEEIGRQQKVAVIHQTKEEQSKIPEGIHVDSDGLITIPTPNKLATLSVRAIPLPEEVTR 764

  Fly   602 DI-DNGDV--------SAKDEFKARARYLSEKYDYDVTEAR-----------------KIWCFGP 640
            .: :|.|:        |:.:|.: ..:.:.:|....:.|.:                 :||.|||
Mouse   765 ILEENSDLIRSMELLTSSLNEGR-NTQAIHQKTQEKIWEFKGKLEKHLTGRKWRNTVDQIWSFGP 828

  Fly   641 DGTGPNFILDCTKSVQYLNEI------------------KDSVVAGFQWASKEGILADENLRGVR 687
            ...|||.::  ::|..:.|.:                  .:|:|:|||.|:..|.:.:|.|.||.
Mouse   829 RKCGPNILV--SRSEDFQNSVWSGPAGRESKEASRFRDFGNSIVSGFQLATLSGPMCEEPLMGVC 891

  Fly   688 FNIYDVTLH-------ADAIHRG------------------------------------------ 703
            |.:....|:       :|..|:|
Mouse   892 FVLEKWELNKCAEQGASDKQHQGQCDLAGEGQGGGKTCHVGDENQEQQDVCSEPFEETSQKGDSP 956

  Fly   704 --------GGQIIPTTRR-CLYAAAITAKP-RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFE 758
                    .||:|.|.:. |.|  |:..|| |||..:|.|:|......:|.:|.||::|.|.|.:
Mouse   957 VIDCYGPFSGQLIATMKEACRY--ALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQ 1019

  Fly   759 ENQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP------------- 810
            |....||.||::||.|||.|||||..::|..|.|.|.||.||.||:|:|.||
Mouse  1020 EEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEVIPSDPFWVPTTEEEYLHF 1084

  Fly   811 -----SEPSSKPYAIVQDTRKRKGL 830
                 ||..::.|  :...||||||
Mouse  1085 GEKADSENQARKY--MNAVRKRKGL 1107

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eEF2	NP_525105.2	PTZ00416	1..844	CDD:240409	330/1130 (29%)
		EF2	20..236	CDD:206672	95/248 (38%)
		EF2_snRNP_like_II	380..474	CDD:293901	38/190 (20%)
		EF2_snRNP_III	489..560	CDD:293918	37/70 (53%)
		aeEF2_snRNP_like_IV	560..732	CDD:238839	57/310 (18%)
		eEF2_snRNP_like_C	728..807	CDD:239763	36/78 (46%)
Efl1	NP_001153144.1	PTZ00416	1..1110	CDD:240409	330/1130 (29%)
		EF2	20..253	CDD:206672	95/248 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	429..496		4/66 (6%)
		EF2_II	505..600	CDD:293913	31/94 (33%)
		EF2_snRNP_III	616..687	CDD:293918	37/70 (53%)
		aeEF2_snRNP_like_IV	687..993	CDD:238839	57/310 (18%)
		eEF2_snRNP_like_C	990..1068	CDD:239763	36/77 (47%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0480
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810