Protein Alignment Cul2 and cul3a

DIOPT Version :10

• Sequence 1: NP_610117.1
• Gene: Cul2 / 35420
• FlyBaseID: FBgn0032956
• Length: 753
• Species: Drosophila melanogaster
• Sequence 2: NP_955985.2
• Gene: cul3a / 324655
• ZFINID: ZDB-GENE-030131-3376
• Length: 774
• Species: Danio rerio
• Alignment Length: 804
• Identity: 207/804 - (25%)
• Similarity: 383/804 - (47%)
• Gaps: 109/804 - (13%)

  Fly     1 MSLKPKIVEFVDVWPRLRCIAESVITLTKVERSVWNTS-------FSDVYTLCVAQPEPMADRLY 58
            |::..|.|.  ::|..|:      ..:.:::|.  |.|       :.:.||:.:   ....::||
Zfish    29 MTMDEKYVN--NIWDLLK------NAIQEIQRK--NNSGLSFEELYRNAYTMVL---HKHGEKLY 80

  Fly    59 GETKHFLEQHVQEMLAKKVLIEGECSHSNGGPDLLQRYYITWMEYSQGIKYLHQLYIYLNQQHIK 123
            ...:..:.:|:...:.:.||      ||... :.||.....|.::...:..:..:.:|:::.:::
Zfish    81 TGLREVVTEHLINKVREDVL------HSLNN-NFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQ 138

  Fly   124 KQKITDTESFYGNLSSDAAEQMEIGELGLDIWRLYMIEY--LSSELVRHILEGIAADRASNGTLD 186
            :..:   |:.|              .|||.|:|..::.|  :...|.:.:|:.||.:|
Zfish   139 QNNV---ENVY--------------NLGLIIFRDQVVRYGCIRDHLRQTLLDMIARER------- 179

  Fly   187 HHRVQIIN-GVIHSFVEVQDYKKTGSLKLYQELFEGPMLEASGAYYTDEANKLLHRCSVSEYMQE 250
              |.:::: |.|.:..::..........:|:|.||.|.|:.|..::..|:.|.|...|.|.|:::
Zfish   180 --RGEVVDRGAIRNACQMLMVLGLEGRSVYEEDFEIPFLDMSAEFFQMESQKFLAENSASVYIKK 242

  Fly   251 VIRILEYESRRAQKFLHVSSLPKLRKECEEKFINDRLGFIY----SECREMVSEERRQDLRNMYV 311
            |...:..|..|....|..|:...:.|..|.:.|:..:..|.    |....|:...:.:||..||.
Zfish   243 VEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLACMYK 307

  Fly   312 VLKPIPDNLKS--ELITTFLDHIKSEGLQTVSALKGENIH-IAFVENMLKVHHKYQELIADVFEN 373
            :...:|:.||:  |.::.:|   :.:|...||. :||..: :.:::.:|.:..::...:.:.|.|
Zfish   308 LFGRVPNGLKTMCECMSWYL---REQGKALVSE-EGEGKNPVDYIQGLLDLKSRFDRFLQESFNN 368

  Fly   374 DSLFLSALDKACASVINRRPTERQPCRSAEYVAKYCDTLLKK--SKTCEAEIDQKLTNNITIFKY 436
            |.||...:.......:|..      .||.||::.:.|..|||  ....|.|::..|...:.:|::
Zfish   369 DRLFKQTIAGDFEYFLNLN------SRSPEYLSLFIDDKLKKGVKGLTEQEVESILDKAMVLFRF 427

  Fly   437 IEDKDVYQKFYSRLLAKRLIHEQSQSMDAEEGMINRLKQACGYEFTNKLHRMFTDISVSVDLNNK 501
            :::|||::::|.:.||:||:..:|.|.|:|:.||::||..||.:||:||..||.|:|:|....::
Zfish   428 MQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDE 492

  Fly   502 FNTHLKDSNVDL-GINLAIKVLQAGAWPLGSTQVIPFA-VPQEFEKSIKMFEDYYHKLFSGRKLT 564
            |..||:.|.|.| |::|.::||..|.||..|  ..|.. :|.....:.::|..:|....|||:||
Zfish   493 FRHHLQTSQVSLCGVDLTVRVLTTGYWPTQS--ATPKCNIPPSPRHAFEVFRRFYLAKHSGRQLT 555

  Fly   565 WLHHMCHGELKLSH---LKK-------------------SYIVTMQTYQMAIILLFETCDSLSCR 607
            ..|||...:|..:.   :||                   .:|:.:.|:||.|::||...:..:..
Zfish   556 LQHHMGSADLNATFYGPIKKEDGSDVGVGGALLTGSNTRKHILQVSTFQMTILMLFNNREKFTFE 620

  Fly   608 EIQNTLQLNDETFQKHMQPIIESK------LLNASSENLAGETRIELNLDYTNK--RTKFKISSA 664
            |||....:.:....:.:|.:...|      .....|:.:.......:|..:|:|  |.|.:..:|
Zfish   621 EIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIESGHVFTVNDQFTSKLHRVKIQTVAA 685

  Fly   665 LQKETPQEVEHTINSVDEDRKLFLQAAIVRIMKARKVLKHNALIQEVLSLSKVSFTPNIAMIKKC 729
            .|.|:..|.:.|...||:|||..::||||||||:||.::||.|:.||....:..|.|:..:|||.
Zfish   686 KQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLRARFLPSPVVIKKR 750

  Fly   730 VESLIDKQYIERTANSGDEYSYMA 753
            :|.||:::|:.||......|:|:|
Zfish   751 IEGLIEREYLARTPEDRKVYTYVA 774

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cul2	NP_610117.1	Cullin	14..652	CDD:459983	161/686 (23%)
		Cullin_Nedd8	683..745	CDD:463146	29/61 (48%)
cul3a	NP_955985.2	Cullin	40..671	CDD:459983	161/686 (23%)
		Cullin_Nedd8	704..766	CDD:463146	29/61 (48%)