DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dap160 and Itsn2

DIOPT Version :9

Sequence 1:	NP_001246118.1	Gene:	Dap160 / 35378	FlyBaseID:	FBgn0023388	Length:	1190	Species:	Drosophila melanogaster
Sequence 2:	NP_001185897.1	Gene:	Itsn2 / 20403	MGIID:	1338049	Length:	1685	Species:	Mus musculus

Alignment Length:	1454	Identity:	444/1454 - (30%)
Similarity:	666/1454 - (45%)	Gaps:	442/1454 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MNSAVDAWAVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGK 65
            ||...:.||:|..||.|:.:||..|:|..|::||.||:.|||||.||..:|.:||||:|.:.|||
Mouse     9 MNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGK 73

  Fly    66 MNINEFSIACKLINLKLRGMDVPKVLPP---------SLLSSL--TGDVPSM------------- 106
            |:..|||||.|||.|||:|..:|.||||         .|:|:.  .|.:|::
Mouse    74 MDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIA 138

  Fly   107 TPRGSTSSLSPLDPLKGIVPAVAPVVPVVAP---PVAVATVISPPGVSVP-SGPTPP-------- 159
            ||..|.:|.:.:.||  ::|  ||:||.|:.   |...|::|.|  :|:| |..|.|
Mouse   139 TPLSSATSGTSIPPL--MMP--APLVPSVSTSSLPNGTASLIQP--LSIPYSSSTLPHASSYSLM 197

  Fly   160 -------------------TSNPPSRHTSISERAPSIESVNQGEWAVQAAQKRKYTQVFNANDRT 205
                               :|:..|...|:|..:|   .....||||....:.||.|.||:.|:.
Mouse   198 MGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSP---KTGTSEWAVPQPSRLKYRQKFNSLDKG 259

  Fly   206 RSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLP 270
            .||||:|.|||..|:||.|.|..||.||||:||||||:|..:||||||.|.:.|.||:.:|:|||
Mouse   260 MSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLP 324

  Fly   271 QEWVPPNLRKIKSRPGSVSGVVSRPGSQPASRHASVSSQSGVGVVDADPTAGLP--GQNKRKENY 333
            .|.|||:.|..| :..||:|.:  |..|...              :.:|...||  .::|||.||
Mouse   325 PELVPPSFRGGK-QVDSVNGTL--PSYQKTQ--------------EEEPQKKLPVTFEDKRKANY 372

  Fly   334 VKGQAELDRRRKIMEDQQRKEREERERKEREEADKREKARLEAERKQQEELERQLQRQREIEMEK 398
            .:|..||::||:::.:||::|.|.:.:||:||.:::::...|.|.|:|.|||::|::|||:|.::
Mouse   373 ERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQR 437

  Fly   399 EEQRKRELEAKEAARKELEKQRQQEWEQARIAEMNAQKEREQERVLKQKAHNTQLNVELSTLNEK 463
            ||:|::|:|.:|||::|||:||:.|||:.|..|:.:||.||||.:::..:....|::||..:|.|
Mouse   438 EEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGK 502

  Fly   464 IKELSQRICDTRAGVTNVKTVIDGMRTQRDTSMSEMSQLKARIKEQNAKLL------QLTQERAK 522
            .:::|.|:.|.:......||.::.:..|.|..:.|:.||:..:||...||:      ||..||.|
Mouse   503 HQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIK 567

  Fly   523 WEAKSKA--SGAALGGENAQQEQLNAAFAHKQLIINQIKDKVENISKEIESKKEDINTNDVQMS- 584
            ....|..  ||.:|         |:...:.|:.:..::|::::.:.||..||..::::.:.|:.
Mouse   568 NMQLSNTPDSGISL---------LHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKC 623

  Fly   585 ------------ELKAELSALITKCEDLYKEYDVQRTSV------------------LELKYNRK 619
                        .|.:.|:.|....::|.:.|:.|:.::                  ||....:|
Mouse   624 GSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKK 688

  Fly   620 NETSVSSAWDTGSSSAWEET-------------------GTTVTDPYAVASNDISALAAPAVDLG 665
            .|...:.....|..:.|.|:                   .|...:..|.|....:|.|
Mouse   689 LEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASA------- 746

  Fly   666 GPAPEGFVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEPGWLAGEINGHTGWFPESYVEKLE 730
                  .|.|:|:|.|.|||.:|::|..||||.|..:...|||||.|...|..||||.:||||:.
Mouse   747 ------LVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVL 805

  Fly   731 VGE--VAPVAAVEAPVDAQVAT---------VADTYN---------------------------- 756
            ..|  ::|..|:..|..:..||         |.|.:|
Mouse   806 SSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVS 870

  Fly   757 ---------DNINTSSI----------------------------------------PAASADLT 772
                     :|:...::                                        |.:...|.
Mouse   871 PIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLI 935

  Fly   773 AAGDV-------------------------------EYYIAAYPYESAEEGDLSFSAGEMVMVIK 806
            ...:|                               |.|||.|.|.|.|.|||:|:.||.::|.:
Mouse   936 PGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQ 1000

  Fly   807 KEGEWWTGTIGSRTGMFPSNYVQKADVGTASTAAAEPVESLDQETTLNGNAAYTAAPVEAQEQVY 871
            |:||||||:||.|||:||||||:..                |||..  |||
Mouse  1001 KDGEWWTGSIGERTGIFPSNYVRPK----------------DQENF--GNA-------------- 1033

  Fly   872 QPLPVQEPSEQPISSPGVGAEEAHEDLDTEVSQINTQSKTQSSEPAESYSRPMSRTSSMTPGMRA 936
                                                 ||:.:|.
Mouse  1034 -------------------------------------SKSGASN--------------------- 1040

  Fly   937 KRSEIAQVIAPYEATSTEQLSLTRGQLIMIRKKTDSGWWEGELQAKGRRRQIGWFPATYVKVLQG 1001
            |:.|||||.:.|.|:.||||||..||||:|.||..||||:|||||:|::||.|||||::||:|
Mouse  1041 KKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLL-- 1103

  Fly  1002 GRNSGRNTPVSGSRIEMTEQILDKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGELNGL 1066
            |.:|.|..|        |...:.:|||:|.|.|.|:|||:|.|..:|:|:.:|:|:||:||.|||
Mouse  1104 GPSSERTMP--------TFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGL 1160

  Fly  1067 SGLFPSNYVGPFVTSEIKPDPGACS------------RRSKG-IQRLWQ-QWRGCDIDQ------ 1111
            :||||||||.  :|::..|....|:            |:.:| |..|.| :.|..|..|
Mouse  1161 TGLFPSNYVK--MTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVF 1223

  Fly  1112 ---AAQHG---DA--------------SNNKLRGIIQAVHITKRRDGQLMGC------------- 1143
               .|:.|   :|              ||.||   ::|:.:.|:..|:.|..
Mouse  1224 QKRMAESGFLTEADMALIFVNWKELIMSNTKL---LRALRVRKKTGGEKMPVQMIGDILAAELSH 1285

  Fly  1144 --AYVRFECNELLAKSMLQENGNQ 1165
              ||:||...:|...::||:..::
Mouse  1286 MQAYIRFCSCQLNGATLLQQKTDE 1309

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dap160	NP_001246118.1	EH	7..101	CDD:197477	51/104 (49%)
		EH	18..83	CDD:238009	36/64 (56%)
		EH	184..279	CDD:197477	54/94 (57%)
		EH	195..261	CDD:238009	39/65 (60%)
		GBP_C	<366..467	CDD:303769	41/100 (41%)
		TACC	369..570	CDD:282818	69/208 (33%)
		coiled coil	432..443	CDD:293879	6/10 (60%)
		coiled coil	457..467	CDD:293879	3/9 (33%)
		TBPIP	<495..589	CDD:284512	25/114 (22%)
		SH3_Intersectin_1	674..728	CDD:212770	27/53 (51%)
		SH3_Intersectin_3	779..830	CDD:212772	32/50 (64%)
		SH3_Intersectin_4	941..998	CDD:212773	37/56 (66%)
		SH3_Intersectin_5	1025..1077	CDD:212774	31/51 (61%)
Itsn2	NP_001185897.1	EH	15..107	CDD:197477	49/91 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..242		7/24 (29%)
		EH	238..317	CDD:197477	45/78 (58%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	335..382		17/63 (27%)
		Smc	350..>747	CDD:224117	111/432 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	716..742		3/25 (12%)
		SH3_Intersectin2_1	747..803	CDD:212921	28/55 (51%)
		INTAP	806..883	CDD:374674	10/76 (13%)
		SH3_Intersectin2_2	883..934	CDD:212923	1/50 (2%)
		SH3_Intersectin2_3	973..1024	CDD:212925	32/50 (64%)
		SH3_Intersectin2_4	1045..1103	CDD:212927	38/57 (67%)
		SH3_Intersectin2_5	1118..1171	CDD:212929	31/54 (57%)
		RhoGEF	1202..1381	CDD:366202	24/111 (22%)
		PH_13	1400..1554	CDD:374702
		C2_Intersectin	1543..1677	CDD:176021
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167844362
Domainoid	1	1.000	176	1.000	Domainoid score	I3602
eggNOG	1	0.900	-	-		E1_KOG1029
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	578	1.000	Inparanoid score	I1028
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45992
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002286
OrthoInspector	1	1.000	-	-		otm43468
orthoMCL	1	0.900	-	-		OOG6_103215
Panther	1	1.100	-	-	O	PTHR11216
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2056
SonicParanoid	1	1.000	-	-		X1514
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.790