DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dap160 and itsn2

DIOPT Version :9

Sequence 1:	NP_001246118.1	Gene:	Dap160 / 35378	FlyBaseID:	FBgn0023388	Length:	1190	Species:	Drosophila melanogaster
Sequence 2:	XP_004918243.2	Gene:	itsn2 / 100127728	XenbaseID:	XB-GENE-1012238	Length:	1771	Species:	Xenopus tropicalis

Alignment Length:	1548	Identity:	443/1548 - (28%)
Similarity:	662/1548 - (42%)	Gaps:	541/1548 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MNSAVDAWAVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGK 65
            ||.....||:|..||.|:.:||.:|:|..|.::|.||:.||:||.||..:|.:||||:|.:.|||
Frog    13 MNGGPSMWAITTEERAKHDKQFASLKPINGLISGDQARSFFIQSGLPSSVLAEIWALSDLNKDGK 77

  Fly    66 MNINEFSIACKLINLKLRGMDVPKVLP-----PSLLSSLT------GDVPSMT------------ 107
            |:..|||||.|||.|||:|..:|.|||     |.:.|.||      |.:|:::
Frog    78 MDQLEFSIAMKLIKLKLQGQSLPLVLPPVMKQPPVFSPLTSSRYGMGSMPNLSMPPPMPSLIPFA 142

  Fly   108 PRGSTSSLSPLDPLKGIVPAVAPVVPVVAPPVAVATVISPPGVS---VPSGPTPPTSNPPSRHTS 169
            |..|.:|:|.|.||    |...|:||.:.     ::...|.|.|   :.|.|.|.:|:....::|
Frog   143 PTASLTSMSSLPPL----PMSTPLVPSLG-----SSSTLPNGTSTALLSSFPVPLSSSTLPHNSS 198

  Fly   170 I------------------------SERAPSIESVNQG-------EWAVQAAQKRKYTQVFNAND 203
            |                        |..:.|..|:...       :|||..|.:.||.|.||:.|
Frog   199 INLLGGGFSSTTMQKTQSLIDLASSSSNSSSTTSLTGNSPKTTYLDWAVPQASRLKYRQKFNSLD 263

  Fly   204 RTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVT 268
            :..:|||||||.:..||||.|....||.||.|:|||.||:|..|||||||:|.:.|.||:.:|:|
Frog   264 KCMTGYLTGSQVKNALVQSSLSHTQLATIWNLADIDKDGKLKADEFILAMYLTDMAKAGQPLPLT 328

  Fly   269 LPQEWVPPNLRKIKSRPGSVSGVVSRP--GSQPA--SRHASVSSQSGVGVVDADPTAGLPGQNKR 329
            ||.:.|||..|      .:||...|.|  |:.|.  ..|.....|.        .||| ..:.||
Frog   329 LPPDLVPPAFR------SNVSITASDPINGTLPLYHQMHEEEPPQK--------KTAG-TFEEKR 378

  Fly   330 KENYVKGQAELDRRRKIMEDQQRKEREERERKEREEADKREKARLEAERKQQEELERQLQRQREI 394
            |||..||..||::||:::.:||::|.|.:.:|:|||.::||:.|.|.|||:..|.||:::||||:
Frog   379 KENIEKGNMELEKRRQVILEQQQREAERKAQKQREEQERRERERQEQERKKLLEQERRMERQREL 443

  Fly   395 EMEKEEQRKRELEAKEAARKELEKQRQQEWEQARIAEMNAQKEREQERVLKQKAHNTQLNVELST 459
            |.::||::::|:|.:|||::|||:|||||.|:.|..|:..|:.||||.::|.|:....|.:||..
Frog   444 ERQREEEKRKEIERREAAKQELERQRQQELERIRRQELFNQRNREQEEIMKLKSKKKSLQLELEA 508

  Fly   460 LNEKIKELSQRICDTRAGVTNVKTVIDGMRTQRDTSMSEMSQLKARIKEQNAKLLQLTQE----- 519
            |.:|.::::.|:.|.|......:|.::.:..:.|..:.|:.||:..::|...||..|..|
Frog   509 LEDKHQQITSRVQDVRGKKQLKRTELETLDRKCDAGIMEIKQLQQELQEYQNKLSILLPEKQFLN 573

  Fly   520 ------------------------------------RAKWEAKSKASGAAL---GGENAQQEQLN 545
                                                |.:.||..|.:.:.|   ...|.|.::|.
Frog   574 EKINQMQAVISNDKSGTGFQGFHKKVSEKDGLCQRLREQLEALEKETSSKLQEMDSFNNQLKELR 638

  Fly   546 AAFAHKQLIINQI---------------------------------KDK----VENISKEIESKK 573
            .:::.:|..::|:                                 ::|    :||:.||.|.|:
Frog   639 GSYSKQQSALHQLQKIKQEKLREAEQKRAEQAQKKQEEEEAARRAKQEKENLWLENLKKEEEEKQ 703

  Fly   574 ---EDINTNDVQMSE-LKAELSALITKCEDLYK------------------------------EY 604
               ||....:.::.| .|||..|...:.|...:                              :.
Frog   704 QRLEDERRREQKLLEKQKAEEEATARQREAQQRLAEEKKRQAEREEEQRKKQQEEEKRRQAQLKK 768

  Fly   605 DVQRTSVLELKYNRKNETS--------VSS--------------AWDTGSSSAWEETGTTVTDPY 647
            |::..|..|.:..|:.|::        :|.              .....||....|..||
Frog   769 DLEEKSKAEDEERRRKESAGRKDSEKRISKLSLDEKFADKLKNIGGRNASSERGREANTT----- 828

  Fly   648 AVASNDISALAAPAVDLGGPAPEGFVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEPGWLAG 712
            :|:.|..|:.||            ||.|.|:|.|.||||:|::|..|||:.|..:...|||||.|
Frog   829 SVSDNRRSSAAA------------FVNYTALYPFEARNADELSFNAGDILQVDEKNIGEPGWLYG 881

  Fly   713 EINGHTGWFPESYVEKLEVGEVAPVAAVEAPVDAQVATVADTYNDNINTSS-------------- 763
            .:.|:.||||.:|.||        ...:|||:..:.|.:..|.  :::|||
Frog   882 CLRGNVGWFPSNYAEK--------ATEIEAPLSPKKALLPPTL--SVSTSSPAKPLLTSKSSSEL 936

  Fly   764 ------IPAASADL--------------------------------------------------- 771
                  :|.:|.::
Frog   937 DTEYQNLPFSSLNVNNTTWQKTSAFTRTVSPGTISPMHGQGQPGESVKAQALCSWTAKKDNHLNF 1001

  Fly   772 -----------------------------------------------------------TAAGDV 777
                                                                       :|||
Frog  1002 SKNDTIVVLEQQENWWFGEVRGQKGWFPKSYVKILPGTDAKTPEPEPVYAAVKKKPVAPSAAG-- 1064

  Fly   778 EYYIAAYPYESAEEGDLSFSAGEMVMVIKKEGEWWTGTIGSRTGMFPSNYVQKADVGTASTAAAE 842
            |.|:|.|.|.|:|.|||.|:.|::::|.:|:||||||.:..|||:||||||:..|
Frog  1065 EEYVALYSYSSSEPGDLIFNEGDLILVTQKDGEWWTGRVEDRTGIFPSNYVRTRD---------- 1119

  Fly   843 PVESLDQETTLNGNAAYTAAPVEAQEQVYQPLPVQEPSEQPISSPGVGAEEAHEDLDTEVSQINT 907
                                                   |.:|| |.|
Frog  1120 ---------------------------------------QEVSS-GAG----------------- 1127

  Fly   908 QSKTQSSEPAESYSRPMSRTSSMTPGMRAKRSEIAQVIAPYEATSTEQLSLTRGQLIMIRKKTDS 972
                              :|.::|     |:.|||||...|.||.||||||..||||:|:||..|
Frog  1128 ------------------KTGTLT-----KKPEIAQVTTAYTATGTEQLSLAPGQLILIQKKNPS 1169

  Fly   973 GWWEGELQAKGRRRQIGWFPATYVKVLQGGRNSGRNTPVSGSRIEMTEQILDKVIALYPYKAQND 1037
            |||:|||||:|::||.|||||::||:|  |.|:.:..|   :.|.:.:     |||:|.|.|.|:
Frog  1170 GWWQGELQARGKKRQKGWFPASHVKLL--GPNNEKAAP---AFIPVCQ-----VIAMYDYIANNE 1224

  Fly  1038 DELSFDKDDIISVLGRDEPEWWRGELNGLSGLFPSNYVGPFVTSEIKPDPGACS----------- 1091
            |||.|.|..:|:||.:|:.:||:||:||::||||||||.  :|:|..|....|:
Frog  1225 DELHFGKGQLINVLNKDDVDWWQGEINGVTGLFPSNYVK--MTTESDPSQQWCADLHTLDTMLPM 1287

  Fly  1092 -RRSKG-IQRLWQ-QWRGCDIDQ---------AAQHG-----------------DASNNKLRGII 1127
             |:.:| |..|.| :.|..|..|         .|:.|                 ..||.||   :
Frog  1288 ERKRQGYIHELIQTEERYMDDLQLVIEVFQKPMAESGLLTEGEMGMIFVNWKELIMSNTKL---L 1349

  Fly  1128 QAVHITKRRDGQLMGC---------------AYVRFECNELLAKSMLQENGNQ 1165
            :|:.:.|:..|:.|..               ||:||...:|...::||...::
Frog  1350 KALRVRKKTGGEKMPVQMIGDILATELSHMPAYIRFCSCQLNGAALLQHKTDE 1402

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dap160	NP_001246118.1	EH	7..101	CDD:197477	50/104 (48%)
		EH	18..83	CDD:238009	34/64 (53%)
		EH	184..279	CDD:197477	51/94 (54%)
		EH	195..261	CDD:238009	37/65 (57%)
		GBP_C	<366..467	CDD:303769	45/100 (45%)
		TACC	369..570	CDD:282818	73/281 (26%)
		coiled coil	432..443	CDD:293879	5/10 (50%)
		coiled coil	457..467	CDD:293879	3/9 (33%)
		TBPIP	<495..589	CDD:284512	29/178 (16%)
		SH3_Intersectin_1	674..728	CDD:212770	26/53 (49%)
		SH3_Intersectin_3	779..830	CDD:212772	28/50 (56%)
		SH3_Intersectin_4	941..998	CDD:212773	38/56 (68%)
		SH3_Intersectin_5	1025..1077	CDD:212774	30/51 (59%)
itsn2	XP_004918243.2	EH	19..111	CDD:197477	46/91 (51%)
		EH	244..323	CDD:197477	43/78 (55%)
		PTZ00121	<351..795	CDD:173412	114/452 (25%)
		tolA_full	617..>823	CDD:274303	30/205 (15%)
		SH3_Intersectin2_1	841..897	CDD:212921	28/55 (51%)
		SH3_Intersectin2_2	984..1035	CDD:212923	0/50 (0%)
		SH3_Intersectin2_3	1066..1117	CDD:212925	28/50 (56%)
		SH3_Intersectin2_4	1138..1196	CDD:212927	39/57 (68%)
		SH3_Intersectin2_5	1211..1264	CDD:212929	30/59 (51%)
		RhoGEF	1291..1474	CDD:238091	24/115 (21%)
		PH_13	1493..1635	CDD:406942
		C2_Intersectin	1635..1769	CDD:176021
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	174	1.000	Domainoid score	I3620
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	584	1.000	Inparanoid score	I990
OMA	1	1.010	-	-		QHG45992
OrthoDB	1	1.010	-	-		D349276at33208
OrthoFinder	1	1.000	-	-		FOG0002286
OrthoInspector	1	1.000	-	-		otm48623
Panther	1	1.100	-	-	O	PTHR11216
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1514
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	10.080