DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG9270 and CFTR

DIOPT Version :9

Sequence 1:	NP_995741.2	Gene:	CG9270 / 35366	FlyBaseID:	FBgn0032908	Length:	1292	Species:	Drosophila melanogaster
Sequence 2:	NP_000483.3	Gene:	CFTR / 1080	HGNCID:	1884	Length:	1480	Species:	Homo sapiens

Alignment Length:	1458	Identity:	417/1458 - (28%)
Similarity:	710/1458 - (48%)	Gaps:	232/1458 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 LQTNPRESAGIFSTLMFCFALPILFKGRKKTLEPTDLYNALKEHKAETLGDKFFATWQSEVRSCG 70
            :|.:|.|.|.:.|.|.|.:..|||.||.::.||.:|:|.......|:.|.:|....|..|:.|
Human     1 MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELAS-- 63

  Fly    71 DSPKKEPSIIRVILKVFGWQLFLSGIVVGVLELGTRATLPLILGALIAEFTRNGNGDGLWAQIYG 135
               ||.|.:|..:.:.|.|:....||.:.:.|: |:|..||:||.:||.:..:...:...|...|
Human    64 ---KKNPKLINALRRCFFWRFMFYGIFLYLGEV-TKAVQPLLLGRIIASYDPDNKEERSIAIYLG 124

  Fly   136 LTLILSILFSVLMFHPLMMGLMHLAMKMRVAVSTAIYRKALRLSRTALGDTTTGQVVNLISNDLG 200
            :.|.|..:...|:.||.:.||.|:.|:||:|:.:.||:|.|:||...|...:.||:|:|:||:|.
Human   125 IGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLN 189

  Fly   201 RFDRALIHFHFLWLGPLELLISSYFLYQQIGVASLYGIVILLLFLPIQTFLSRLTSRLRHQTALR 265
            :||..|...||:|:.||::.:....:::.:..::..|:..|::....|..|.|:..:.|.|.|.:
Human   190 KFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAGLGRMMMKYRDQRAGK 254

  Fly   266 TDQRVRMMNEIISGIQVIKMYTWEKPFGRLIERLRRSEMSSIRKVNYIRGTLLSFEITLSRIAIF 330
            ..:|:.:.:|:|..||.:|.|.||:...::||.||::|:...||..|:|....|.........:|
Human   255 ISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAYVRYFNSSAFFFSGFFVVF 319

  Fly   331 VSLLGFVLMGGELTAERAFSVTAFYNILRRTVCKFFPSGMSQFAEMMVTLRRIKGFMMRSE---- 391
            :|:|.:.|:.| :...:.|:..:|..:||..|.:.||..:..:.:.:..:.:|:.|:.:.|
Human   320 LSVLPYALIKG-IILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTL 383

  Fly   392 ------TEA---------------LYLKGGQTN---KLFEGEPLVKLQSFQARWNHDHVEPVLEN 432
                  ||.               |:.|..|.|   |...|:..:...:|.....     |||::
Human   384 EYNLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGT-----PVLKD 443

  Fly   433 INISLSPPQLVAVIGPVGSGKSSLIQAILGELPGESGKLKVQGDISYASQEPWLFNASVRDNILF 497
            ||..:...||:||.|..|:||:||:..|:|||....||:|..|.||:.||..|:...::::||:|
Human   444 INFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIF 508

  Fly   498 GLPMDKHRYRNVIRNCALERDF-ELLHGDRTFVGERGASLSGGQRARISLARAVYRQADTYLLDD 561
            |:..|::|||:||:.|.||.|. :....|...:||.|.:||||||||||||||||:.||.||||.
Human   509 GVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDS 573

  Fly   562 PLSAVDTHVGRHLFEECMRGFLRDKLVILVTHQLQFLEHADLIVIMDKGKISAVGTYEEMLKSGQ 626
            |...:|....:.:||.|:...:.:|..||||.:::.|:.||.|:|:.:|.....||:.|:.....
Human   574 PFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQP 638

  Fly   627 DFGKLLATEAQEMGDSNQEQVNA----------------EGDSRNDKSTYSRQSSR--------- 666
            ||...|      ||..:.:|.:|                |||:....:...:||.:
Human   639 DFSSKL------MGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTETKKQSFKQTGEFGEKR 697

  Fly   667 ---------------------------------------------------VSRVSVTS------ 674
                                                               :.|:||.|
Human   698 KNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGPTLQ 762

  Fly   675 -------VDSSTESILDNERQPAQESRSQGKIGLG---------IYGKYFSAGSG---------- 713
                   ::..|.|:...:....:.:.|..|:.|.         ||.:..|..:|
Human   763 ARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVSLAPQANLTELDIYSRRLSQETGLEISEEINEE 827

  Fly   714 -------------------------------------WLMVI--------LVAFFCLGTQILASG 733
                                                 |.:||        ||..:.||...|...
Human   828 DLKECFFDDMESIPAVTTWNTYLRYITVHKSLIFVLIWCLVIFLAEVAASLVVLWLLGNTPLQDK 892

  Fly   734 GDYFLSYWVKNN-------DSSSSTDIYIFSGINAALVIFALLRTLLFFSMAMHSSTQLHNTMFQ 791
            |:   |...:||       .:||....||:.|:...|:.....|.|......:..|..||:.|..
Human   893 GN---STHSRNNSYAVIITSTSSYYVFYIYVGVADTLLAMGFFRGLPLVHTLITVSKILHHKMLH 954

  Fly   792 GVSRTALYFFHANPSGRILNRFAMDLGQVDEILPAVMLDCIQIFLTISGIIGVLCITNPWYLINT 856
            .|.:..:...:...:|.|||||:.|:..:|::||..:.|.||:.|.:.|.|.|:.:..|:..:.|
Human   955 SVLQAPMSTLNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVAT 1019

  Fly   857 ITMFLAFHFLRTFYLSTSRDLKRLEAIARSPMYSHFSATLNGLSTIRAMEAQDLLTKEYDNYQDI 921
            :.:.:||..||.::|.||:.||:||:..|||:::|...:|.||.|:||...|......:....::
Human  1020 VPVIVAFIMLRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNL 1084

  Fly   922 HSSGYYTFLSTNRAFGYYLDLFCVAYVISVTLMSYFNPPVDNPGQIGLVITQAMSMTGTVQWGMR 986
            |::.::.:|||.|.|...:::..|.:.|:||.:|..... :..|::|:::|.||::..|:||.:.
Human  1085 HTANWFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTG-EGEGRVGIILTLAMNIMSTLQWAVN 1148

  Fly   987 QSAELENSMTSVERVLEYRHLEAEGE------------------FESPDDKKPPMNWPQEGLISA 1033
            .|.::::.|.||.||.::..:..||:                  .|:...||..: ||..|.::.
Human  1149 SSIDVDSLMRSVSRVFKFIDMPTEGKPTKSTKPYKNGQLSKVMIIENSHVKKDDI-WPSGGQMTV 1212

  Fly  1034 EQLSLRYNPDPKADRVLKSLNFIIMPREKIGIVGRTGAGKSSLINALFRLSYNEGSLVIDNTDIL 1098
            :.|:.:|.....|  :|::::|.|.|.:::|::||||:|||:|::|..||...||.:.||.....
Human  1213 KDLTAKYTEGGNA--ILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEIQIDGVSWD 1275

  Fly  1099 GIGLHDLRSKISIIPQEPVLFSGTLRCNLDPFEQYADEKLWEALEEVHLKDEVSELPNGLESVVA 1163
            .|.|...|....:|||:..:||||.|.||||:||::|:::|:..:||.|:..:.:.|..|:.|:.
Human  1276 SITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLV 1340

  Fly  1164 EGGSNYSVGQRQLVCLARAILRENRILVMDEATANVDPQTDALIQSTIRRKFRDCTVLTIAHRLN 1228
            :||...|.|.:||:||||::|.:.:||::||.:|::||.|..:|:.|:::.|.||||:...||:.
Human  1341 DGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEHRIE 1405

  Fly  1229 TIIDSDRVMVLDAGTLVEFGSPFELLTQ 1256
            .:::..:.:|::...:.::.|..:||.:
Human  1406 AMLECQQFLVIEENKVRQYDSIQKLLNE 1433

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG9270	NP_995741.2	CFTR_protein	6..1257	CDD:273530	417/1458 (29%)
		ABC_membrane	92..362	CDD:294371	83/269 (31%)
		ABCC_MRP_domain1	412..611	CDD:213217	84/199 (42%)
		ABC_membrane	718..981	CDD:294371	80/277 (29%)
		ABCC_MRP_domain2	1029..1250	CDD:213211	78/220 (35%)
CFTR	NP_000483.3	CFTR_protein	1..1480	CDD:273530	417/1458 (29%)
		ABC_membrane	85..303	CDD:279056	72/218 (33%)
		ABCC_CFTR1	389..670	CDD:213258	103/291 (35%)
		CFTR_R	639..849	CDD:291094	25/215 (12%)
		Disordered R region. /evidence=ECO:0000269\|PubMed:10792060	654..831		19/176 (11%)
		ABC_membrane	866..1147	CDD:279056	84/284 (30%)
		ABCC_CFTR2	1208..1480	CDD:213256	81/228 (36%)
		Interaction with GORASP2. /evidence=ECO:0000269\|PubMed:21884936	1386..1480		12/48 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1452..1480
		PDZ-binding. /evidence=ECO:0000269\|PubMed:11707463, ECO:0000269\|PubMed:16331976	1478..1480
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165151594
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.850