Protein Alignment vari and Pals1

DIOPT Version :10

• Sequence 1: NP_724288.3
• Gene: vari / 35343
• FlyBaseID: FBgn0250785
• Length: 636
• Species: Drosophila melanogaster
• Sequence 2: NP_062525.1
• Gene: Pals1 / 56217
• MGIID: 1927339
• Length: 675
• Species: Mus musculus
• Alignment Length: 688
• Identity: 193/688 - (28%)
• Similarity: 328/688 - (47%)
• Gaps: 139/688 - (20%)

  Fly     7 RSRRQARQDKVEL----------LARNNKVNGED----DTSDNAAFRN---STDLSDHDEIF--L 52
            |.||:....|.||          ||:.....|.|    ||.:.....:   :.::.|.:::|  |
Mouse    67 RRRREEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPIFDTEEGIVLESPHYAVNILDVEDLFSSL 131

  Fly    53 KGLLRS-----------------------NSNTPHKELMLNPTEPQPVPLFLPAHLNNKPICDDI 94
            |.:..:                       |:...|..:.::.::..|.   .|...|.:.:..::
Mouse   132 KHIQHTLVDSQSQEDISLLLQLVQNRDFQNAFKIHNAVTVHMSKASPP---FPLIANVQDLVQEV 193

  Fly    95 IRKFSPSRRLESRELAKLLAQPHFRALLRAHDEIGALYEQRLKAAGGSTSQLE-IASQR------ 152
            .....|..:.|.:||..||..||.:|||.|||::.   ||.:        ||| |..:|
Mouse   194 QTVLKPVHQKEGQELTALLNAPHIQALLLAHDKVA---EQEM--------QLEPITDERVYESIG 247

  Fly   153 QTGGYLFTEDVLNTKMPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHV 217
            ..||              ||:|:|.:.:....|||.||. :|...::::||:.||..:|..:||.
Mouse   248 HYGG--------------ETVKIVRIEKARDIPLGATVR-NEMDSVIISRIVKGGAAEKSGLLHE 297

  Fly   218 GDVILEVNGTPVRTPDELQV--EVSRAKENLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLET 280
            ||.:||:||..:|..|..:|  .:|.....||..:.|:  ::||.                  ..
Mouse   298 GDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPS--QQIKP------------------PP 342

  Fly   281 GKKLTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDK--IGLI 343
            .|:...:::|.|.|:||:|..:|||::||.|:.||||.:|:.:|||||||.....|.::  .||:
Mouse   343 AKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLV 407

  Fly   344 PSQELEERRKAF--VAPEADYVHKIG--ICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVT-- 402
            |.:..:::|:|.  ...|.....|.|  .|..:..|:::|.:|.:..|.::|..|:|.|||::
Mouse   408 PGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLY 472

  Fly   403 RMPPFRRKTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEME 467
            ..|..|::.::|||....|:..|:.||:|.:.|:|.:.:|||:|.:|..|..|..|.|:.|:..|
Mouse   473 HQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASAVPHTTRNRRDHEVAGRDYHFVSRQAFE 537

  Fly   468 EAVRNNEFLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPG 532
            ..:...:|:|:||...|||||.:.|::.|||||::|:|.....:||.|.|| :|.|::||:|.|.
Mouse   538 ADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNS-DLKPYIIFIAPPS 601

  Fly   533 MEQLKTIYADRRATGSNRNLSFDRQSSIRFSSRRARTLESLASLYEDDDLVATVEESSFVQRKYE 597
            .|:|:.:.|..   |.|.                           :.::|...:|::..:::...
Mouse   602 QERLRALLAKE---GKNP---------------------------KPEELREIIEKTREMEQNNG 636

  Fly   598 KYFDMVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWI 635
            .|||..|||.|.|:.:::::..::::..|.||||..|:
Mouse   637 HYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWL 674

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
vari	NP_724288.3	L27	<105..128	CDD:460717	13/22 (59%)
		PDZ_MPP6-MPP2-like	174..252	CDD:467268	27/79 (34%)
		SH3_MPP	287..347	CDD:212796	28/61 (46%)
		Guanylate_kin	408..622	CDD:395500	65/213 (31%)
Pals1	NP_062525.1	Required for the correct localization of PALS1 and PATJ at cell-cell contacts and the normal formation of tight junctions and adherens junctions. /evidence=ECO:0000269|PubMed:17182851	1..345		76/326 (23%)
		Interaction with PARD6B. /evidence=ECO:0000269|PubMed:15140881	21..140		16/72 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	51..79		4/11 (36%)
		L27_N	123..170	CDD:462666	6/46 (13%)
		Interaction with LIN7C. /evidence=ECO:0000269|PubMed:10753959	181..243		23/72 (32%)
		L27	189..237	CDD:197794	19/58 (33%)
		PDZ_MPP5-like	255..334	CDD:467259	27/79 (34%)
		SH3_MPP5	349..411	CDD:212969	28/61 (46%)
		GuKc	491..661	CDD:214504	61/200 (31%)