Protein Alignment vari and pals2a

DIOPT Version :9

• Sequence 1: NP_724288.3
• Gene: vari / 35343
• FlyBaseID: FBgn0250785
• Length: 636
• Species: Drosophila melanogaster
• Sequence 2: NP_001243118.1
• Gene: pals2a / 402878
• ZFINID: ZDB-GENE-050506-35
• Length: 550
• Species: Danio rerio
• Alignment Length: 610
• Identity: 238/610 - (39%)
• Similarity: 346/610 - (56%)
• Gaps: 98/610 - (16%)

  Fly    41 STDLSDHDEIFLKGLLRS----NSNTPHKELMLNPTEPQPVPLFLPAHLNNKPICDDIIRKFSP- 100
            ||...|.|.|||:|::.|    :....|:.|       :.|.| .....||..:..:|:...|.
Zfish    25 STGAKDIDLIFLRGIMESPIVRSLAKAHERL-------EDVKL-EAVQSNNVELVSEILSDMSSL 81

  Fly   101 -SRRLESRELAKLLAQPHFRALLRAHDEIGALYEQRLKAAGGSTSQLEIASQRQTGGYLFTEDVL 164
             .|...:.||:.:|.:|||::||.|||::.:   :..:|...||:...::|
Zfish    82 IIRDESAAELSNILKEPHFQSLLEAHDKVAS---KSYEAPPTSTNSTSMSS-------------- 129

  Fly   165 NTKMPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPV 229
            ::.||.:|::|:.:::...:|||:|..::| ..||:||:|.|.:||:|.|||.||||.||||..|
Zfish   130 SSLMPADTVRMISIQKKAGEPLGVTFRVEE-GDLVIARVLHGSMIDRQGMLHAGDVIREVNGREV 193

  Fly   230 -RTPDELQVEVSRAKENLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFT 293
             :.|..||..:.....::||||.|:..:       |....||                |::..|.
Zfish   194 GKDPMALQHMLRDCNGSITLKILPSYKD-------TPPPAQV----------------YLKPHFN 235

  Fly   294 YNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAP 358
            |||:.|:|:|||:.||.|..||||.|:|.:|||||||.||  .....||||||.|||:|||||..
Zfish   236 YNPNTDNLIPCREAGLAFSKGDILHIVNKEDPNWWQACNI--NGGHTGLIPSQFLEEKRKAFVRR 298

  Fly   359 EADYVHKIG---ICGTRI-SKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRRKTLVLIGVSG 419
            :.|     |   :|||.. .|:|:|.||.:..|.|||:.||.:||||.:||||:||||||||..|
Zfish   299 DLD-----GSGILCGTLTGKKKKKKMMYLTAKNAEFDRHELQIYEEVAKMPPFQRKTLVLIGAQG 358

  Fly   420 VGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGN 484
            ||||:||||||..:..::|..:|.|||..|..|::|.||.|:.|||||..::.:.:||:||::||
Zfish   359 VGRRSLKNRLIVLNPLRYGTTVPFTSRRPRDDEKDGQSYCFVSREEMEMDIKASRYLEHGEYDGN 423

  Fly   485 LYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIY---ADRRAT 546
            ||||.:.||.:|:.:||.||||..|.|||:|..| |.||||:|:|||.::.|:.::   .|...|
Zfish   424 LYGTKMDSIHEVVRAGRTCILDVNPQALKVLKTS-EFMPFVVFIAAPELDTLRAMHKAVVDAGIT 487

  Fly   547 GSNRNLSFDRQSSIRFSSRRARTLESLASLYEDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDE 611
                                       ..|..:.||..||:||:.::|.|..|||:.|||::.|:
Zfish   488 ---------------------------TKLLTETDLKKTVDESARIKRAYNHYFDLTIVNDNLDK 525

  Fly   612 TFRQVVETLDQMSHEEQWVPVNWIY 636
            .|.::...::|::.:.|||||||:|
Zfish   526 AFEKLQAAVEQLTTQPQWVPVNWVY 550

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
vari	NP_724288.3	L27	<105..128	CDD:280918	11/22 (50%)
		PDZ_signaling	173..250	CDD:238492	32/77 (42%)
		SH3_MPP	287..347	CDD:212796	33/59 (56%)
		Guanylate_kin	408..622	CDD:279019	89/216 (41%)
		GuKc	418..622	CDD:214504	81/206 (39%)
pals2a	NP_001243118.1	L27	13..66	CDD:197794	13/48 (27%)
		L27	68..119	CDD:308467	15/53 (28%)
		PDZ_signaling	138..216	CDD:238492	33/78 (42%)
		SH3_MPP	229..286	CDD:212796	32/58 (55%)
		Guanylate_kin	347..537	CDD:331880	89/217 (41%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170594318
Domainoid	1	1.000	60	1.000	Domainoid score	I10546
eggNOG	1	0.900	-	-		E1_COG0194
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H22976
Inparanoid	1	1.050	396	1.000	Inparanoid score	I1911
OMA	1	1.010	-	-		QHG48629
OrthoDB	1	1.010	-	-		D364535at33208
OrthoFinder	1	1.000	-	-		FOG0003013
OrthoInspector	1	1.000	-	-		otm25584
orthoMCL	1	0.900	-	-		OOG6_104327
Panther	1	1.100	-	-	O	PTHR23122
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2005
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.770