DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment vari and Pals2

DIOPT Version :9

Sequence 1:	NP_724288.3	Gene:	vari / 35343	FlyBaseID:	FBgn0250785	Length:	636	Species:	Drosophila melanogaster
Sequence 2:	XP_006236558.1	Gene:	Pals2 / 362359	RGDID:	1311833	Length:	554	Species:	Rattus norvegicus

Alignment Length:	620	Identity:	237/620 - (38%)
Similarity:	346/620 - (55%)	Gaps:	101/620 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    40 NSTDLSDHDEIFLKGLLRSNSNTPHKELMLNPTEPQPVPLFLPAHL------------NNKPICD 92
            :||...:.|.|||||            :|.||.    |.....||.            ||..:.:
  Rat    13 SSTGAEEIDLIFLKG------------IMENPI----VKSLAKAHERLEDSKLEAVSDNNLELVN 61

  Fly    93 DIIRKFSPSRRLESR--ELAKLLAQPHFRALLRAHDEIGA-LYEQRLKAAGGSTSQLEIASQRQT 154
            :|:...:|...::..  ||..:|.:|||::||.|||.:.: .||     :..|:.::.|.|
  Rat    62 EILEDITPLISVDENVAELVGILKEPHFQSLLEAHDIVASKCYE-----SPPSSPEMNIPS---- 117

  Fly   155 GGYLFTEDVLNTKMPVETIKMVGLRRDPSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGD 219
                    :.|..:||:.|:::|:.:...:|||:|..: |...||:||||.||:||:|.:|||||
  Rat   118 --------LNNQLLPVDAIRILGIHKRAGEPLGVTFRV-ENNDLVIARILHGGMIDRQGLLHVGD 173

  Fly   220 VILEVNGTPV-RTPDELQVEVSRAKENLTLKIGPNVDEEIKSGRYTVS----GGQVKQNGIASLE 279
            :|.||||..| ..|.|||..:.....::||||.|:..:.|...:..|:    ...|:|
  Rat   174 IIKEVNGHEVGNNPKELQELLKNISGSVTLKILPSYRDTIAPQQSYVNMERHPAHVRQ------- 231

  Fly   280 TGKKLTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIP 344
                  .:::..|.|||..|:|:||::.||.|..|:||||:|.:|||||||.:: .|....||||
  Rat   232 ------VFVKCHFDYNPFNDNLIPCKEAGLKFSKGEILQIVNREDPNWWQASHV-KEGGSAGLIP 289

  Fly   345 SQELEERRKAFVAPEADYVHKIGICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRR 409
            ||.|||:|||||  ..|:.:....|||..:|:|:|.||.:..|.|||:.|:.:||||.:||||:|
  Rat   290 SQFLEEKRKAFV--RRDWDNSGPFCGTISNKKKKKMMYLTTRNAEFDRHEIQIYEEVAKMPPFQR 352

  Fly   410 KTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNE 474
            |||||||..|||||:||||.|..:..:||..:|.|||..|..|::|.:|.|:.|.|||..::..:
  Rat   353 KTLVLIGAQGVGRRSLKNRFIVLNPARFGTTVPFTSRKPREDEKDGQAYKFVSRSEMEADIKAGK 417

  Fly   475 FLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTI 539
            :||:||:.||||||.:.||.||:.:||.||||..|.|||:|..| |.||:|:|:|||.:|.|:.:
  Rat   418 YLEHGEYEGNLYGTKIGSILDVVQTGRTCILDVNPQALKVLRTS-EFMPYVVFIAAPELETLRAM 481

  Fly   540 Y---ADRRATGSNRNLSFDRQSSIRFSSRRARTLESLASLYEDDDLVATVEESSFVQRKYEKYFD 601
            :   .|...|                           ..|..|.||..||:||:.:||.|..|||
  Rat   482 HKAVVDAGIT---------------------------TKLLTDSDLKKTVDESARIQRAYNHYFD 519

  Fly   602 MVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWIY 636
            ::|||::.|:.|..:...::::..|.||||::|:|
  Rat   520 LIIVNDNLDKAFENLQTAIEKLRTEPQWVPISWVY 554

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vari	NP_724288.3	L27	<105..128	CDD:280918	11/24 (46%)
		PDZ_signaling	173..250	CDD:238492	35/77 (45%)
		SH3_MPP	287..347	CDD:212796	30/59 (51%)
		Guanylate_kin	408..622	CDD:279019	90/216 (42%)
		GuKc	418..622	CDD:214504	82/206 (40%)
Pals2	XP_006236558.1	L27	2..55	CDD:197794	14/57 (25%)
		L27	57..108	CDD:397114	15/55 (27%)
		PDZ_signaling	133..206	CDD:238492	34/73 (47%)
		SH3_MPP6	233..293	CDD:212971	31/60 (52%)
		Guanylate_kin	351..541	CDD:395500	90/217 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166352373
Domainoid	1	1.000	61	1.000	Domainoid score	I10199
eggNOG	1	0.900	-	-		E1_COG0194
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H22976
Inparanoid	1	1.050	393	1.000	Inparanoid score	I1909
OMA	1	1.010	-	-		QHG48629
OrthoDB	1	1.010	-	-		D364535at33208
OrthoFinder	1	1.000	-	-		FOG0003013
OrthoInspector	1	1.000	-	-		otm45915
orthoMCL	1	0.900	-	-		OOG6_104327
Panther	1	1.100	-	-	LDO	PTHR23122
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X2005
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	14	13.810