DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment vari and Pals1

DIOPT Version :9

Sequence 1:	NP_724288.3	Gene:	vari / 35343	FlyBaseID:	FBgn0250785	Length:	636	Species:	Drosophila melanogaster
Sequence 2:	NP_001101504.1	Gene:	Pals1 / 314259	RGDID:	1308071	Length:	675	Species:	Rattus norvegicus

Alignment Length:	594	Identity:	181/594 - (30%)
Similarity:	300/594 - (50%)	Gaps:	103/594 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    59 NSNTPHKELMLNPTEPQPVPLFLPAHLNNKPICDDIIRKFSPSRRLESRELAKLLAQPHFRALLR 123
            |:.|.|    :|...| |.||.    .|.:.:..::.....|..:.|.:||..||..||.:|||.
  Rat   167 NAVTVH----MNKASP-PFPLI----ANVQDLVQEVQTVLKPVHQKEGQELTALLNAPHIQALLL 222

  Fly   124 AHDEIGALYEQRLKAAGGSTSQLE-IASQR------QTGGYLFTEDVLNTKMPVETIKMVGLRRD 181
            |||::.   ||.:        ||| |..:|      ..||              ||:|:|.:.:.
  Rat   223 AHDKVA---EQEM--------QLEPITDERVYESIGHYGG--------------ETVKIVRIEKA 262

  Fly   182 PSKPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQV--EVSRAKE 244
            ...|||.||. :|...::::||:.||..:|..:||.||.:||:||..:|..|..:|  .:|....
  Rat   263 RDIPLGATVR-NEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHG 326

  Fly   245 NLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLPCRDIGL 309
            .||..:.|:  ::||.                  ...|:...:::|.|.|:||:|..:|||::||
  Rat   327 TLTFVLIPS--QQIKP------------------PPAKETVIHVKAHFDYDPSDDPYVPCRELGL 371

  Fly   310 PFKSGDILQIINVKDPNWWQAKNITAESDK--IGLIPSQELEERRKAF--VAPEADYVHKIG--I 368
            .|:.||||.:|:.:|||||||.....|.::  .||:|.:..:::|:|.  ...|.....|.|  .
  Rat   372 SFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEPEKSGKLW 436

  Fly   369 CGTRISKRKRKTMYRSVANCEFDKAELLLYEEVT--RMPPFRRKTLVLIGVSGVGRRTLKNRLIN 431
            |..:..|:::|.:|.:..|.::|..|:|.|||::  ..|..|::.::|||....|:..|:.||:|
  Rat   437 CAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMN 501

  Fly   432 SDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGNLYGTHLQSIKDV 496
            .:.|:|.:.:|||:|.:|..|..|..|.|:.|:..|..:...:|:|:||...|||||.:.|::.|
  Rat   502 KEKDRFASAVPHTTRNRRDHEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQV 566

  Fly   497 INSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIYADRRATGSNRNLSFDRQSSIR 561
            ||||::|:|.....:||.|.|| :|.|::||:|.|..|:|:.:.|..   |.|.
  Rat   567 INSGKICLLSLRAQSLKTLRNS-DLKPYIIFIAPPSQERLRALLAKE---GKNP----------- 616

  Fly   562 FSSRRARTLESLASLYEDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDETFRQVVETLDQMSHE 626
                            :.::|...:|::..:::....|||..|||.|.|:.:::::..::::..|
  Rat   617 ----------------KPEELREIIEKTREMEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTE 665

  Fly   627 EQWVPVNWI 635
            .||||..|:
  Rat   666 PQWVPSTWL 674

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vari	NP_724288.3	L27	<105..128	CDD:280918	13/22 (59%)
		PDZ_signaling	173..250	CDD:238492	27/78 (35%)
		SH3_MPP	287..347	CDD:212796	28/61 (46%)
		Guanylate_kin	408..622	CDD:279019	65/213 (31%)
		GuKc	418..622	CDD:214504	61/203 (30%)
Pals1	NP_001101504.1	Required for the correct localization of PALS1 and PATJ at cell-cell contacts and the normal formation of tight junctions and adherens junctions. /evidence=ECO:0000250\|UniProtKB:Q9JLB2	1..345		64/232 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..32
		Interaction with PARD6B. /evidence=ECO:0000250\|UniProtKB:Q9JLB2	21..140
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	52..79
		L27_N	123..170	CDD:401122	1/2 (50%)
		Interaction with LIN7C. /evidence=ECO:0000250\|UniProtKB:Q9JLB2	181..243		24/76 (32%)
		L27	189..237	CDD:197794	19/58 (33%)
		PDZ_signaling	254..333	CDD:238492	27/79 (34%)
		SH3_MPP5	349..411	CDD:212969	28/61 (46%)
		GuKc	491..661	CDD:214504	61/200 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0194
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810