DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment vari and MAGI3

DIOPT Version :9

Sequence 1:	NP_724288.3	Gene:	vari / 35343	FlyBaseID:	FBgn0250785	Length:	636	Species:	Drosophila melanogaster
Sequence 2:	NP_001136254.1	Gene:	MAGI3 / 260425	HGNCID:	29647	Length:	1481	Species:	Homo sapiens

Alignment Length:	645	Identity:	132/645 - (20%)
Similarity:	208/645 - (32%)	Gaps:	238/645 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly    69 LNPTEPQPVPLFLPAHLNNKPICDDIIRKFSPSRRLESRELAKLLAQPHFRALLRAHDEIGALYE 133
            :|...|||:| |.|:          |||..||  :|:..|:           .|::.    .|||
Human   680 INEPIPQPMP-FPPS----------IIRSGSP--KLDPSEV-----------YLKSK----TLYE 716

  Fly   134 QRLKAAGGSTSQLEIASQRQTGGYLFTEDVLNTKMPVETIKMVGLRRDPSKPLGLTVELDEFKQL 198
            .:    ..:|..|::..::|..|:.|.  ||....|.::|
Human   717 DK----PPNTKDLDVFLRKQESGFGFR--VLGGDGPDQSI------------------------- 750

  Fly   199 VVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQV--EVSRAKEN----LTLK----IGPN 253
            .:..|:..|..:|...|...|.::.::|.||:.....||  .::.|..|    ||::    .|..
Human   751 YIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEK 815

  Fly   254 VDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLPCR-------DIGLPF 311
            ..|:..|..:..:     |||...|                |.:|   :|.|       |:.|..
Human   816 QPEDDSSQAFIST-----QNGSPRL----------------NRAE---VPARPAPQEPYDVVLQR 856

  Fly   312 KSGD-----ILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAPEADYVHKIG---- 367
            |..:     ||...|...|               |:||                   ||||
Human   857 KENEGFGFVILTSKNKPPP---------------GVIP-------------------HKIGRVIE 887

  Fly   368 ------------------ICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPP----FRRK 410
                              :.|..|.:.....:.:.:.:........::.||....||    ..|:
Human   888 GSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQ 952

  Fly   411 TLVL----IGVSGV----GRRTLKNRLINSDV---------DKFGAVIPHTSRPKRAL------- 451
            :..|    :|.|..    |.|:.....|..||         |.     .|.::|..|:
Human   953 SPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDH-----KHLAQPDTAVISVVGSR 1012

  Fly   452 -EENGSSYWFMDREEMEEAVRNNEFLEYG--EHNGNLYGTHLQSIKDVINSGRMCILDCAPNALK 513
             .:|...|    ..|:|...|...|...|  |:|..|:...|......|..||:.:.|      :
Human  1013 HNQNLGCY----PVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGD------Q 1067

  Fly   514 ILHNSQELMPFVIFVAAPGMEQL---KTIYADRRATG-------------SNRNLSFDRQSSIRF 562
            |:..:.|....:....|..:.|.   |.:...|..||             |:.|:.:|.||.:..
Human  1068 IVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDINNPSSSNVIYDEQSPLPP 1132

  Fly   563 SSRRARTLESLASLYEDDDLVATVEESSFVQ--RKYEKYFDMV-----IVNEDFDETFRQ 615
            ||.       .||::|:.. |..:|||..||  .|.|:..|:|     .:||:..|...|
Human  1133 SSH-------FASIFEESH-VPVIEESLRVQICEKAEELKDIVPEKKSTLNENQPEIKHQ 1184

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vari	NP_724288.3	L27	<105..128	CDD:280918	2/22 (9%)
		PDZ_signaling	173..250	CDD:238492	15/86 (17%)
		SH3_MPP	287..347	CDD:212796	14/71 (20%)
		Guanylate_kin	408..622	CDD:279019	60/258 (23%)
		GuKc	418..622	CDD:214504	57/244 (23%)
MAGI3	NP_001136254.1	Interaction with ADRB1 and TGFA. /evidence=ECO:0000250	18..106
		PDZ_signaling	24..101	CDD:238492
		NK	123..>197	CDD:327404
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	182..273
		WW	294..326	CDD:197736
		WW	342..372	CDD:238122
		Interaction with PTEN. /evidence=ECO:0000269\|PubMed:10748157	410..492
		PDZ	413..493	CDD:214570
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	542..570
		PDZ	575..656	CDD:214570
		Interaction with ADGRB1. /evidence=ECO:0000269\|PubMed:10748157	726..808		21/108 (19%)
		PDZ_signaling	726..807	CDD:238492	21/107 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	821..843		8/45 (18%)
		PDZ_signaling	849..935	CDD:238492	16/119 (13%)
		Interaction with LPAR2 and GRIN2B. /evidence=ECO:0000269\|PubMed:10748157, ECO:0000269\|PubMed:16904289	851..938		16/120 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..976		8/36 (22%)
		PDZ_signaling	1022..1100	CDD:238492	18/83 (22%)
		Red1	<1132..1461	CDD:311769	19/61 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1170..1481		4/15 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0194
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810