DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment vari and pals1b

DIOPT Version :9

Sequence 1:	NP_724288.3	Gene:	vari / 35343	FlyBaseID:	FBgn0250785	Length:	636	Species:	Drosophila melanogaster
Sequence 2:	NP_001171218.2	Gene:	pals1b / 100422734	ZFINID:	ZDB-GENE-060531-2	Length:	639	Species:	Danio rerio

Alignment Length:	651	Identity:	185/651 - (28%)
Similarity:	309/651 - (47%)	Gaps:	126/651 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 NGEDDTSDNAAFRNSTDL------------------SDHD-EIFLKGLLRSNSNTPHKELMLNPT 72
            :|.:.|..::..:::|||                  |..| |:.|..||::|.   .:..::..:
Zfish    70 DGMNITEASSEEKSTTDLDSVLQCLHHLHQCVLDEESQQDVELLLDLLLQTNF---QQAFIMQRS 131

  Fly    73 EPQ------PVPLFLPAHLNNKPICDDIIRKFSPSRRLESRELAKLLAQPHFRALLRAHDEIGAL 131
            ..|      |.|..|.||.  :.:...:......|:..|:.||..||..||.:||:.|||   .:
Zfish   132 VAQGMSRLCPPPYPLTAHA--QQLRGQVESVLQSSKHTEATELTGLLTSPHLQALMEAHD---CI 191

  Fly   132 YEQRLKAAGGSTSQLEIASQRQTGGYLFTEDVLNTKMPVETIKMVGLRRDPSKPLGLTVELDEFK 196
            .||.|     ....:|...|.:                 :|.|:|.|.:....|||:||. :|..
Zfish   192 AEQEL-----DVDTIEAVDQDE-----------------KTTKLVSLEKIRDIPLGVTVR-NEQD 233

  Fly   197 QLVVARILAGGVIDKQSMLHVGDVILEVNGTPVR--TPDELQVEVSRAKENLTLKIGPNVDEEIK 259
            .:|::|:::||..:|.::|..||.|||:|..|||  |.:::...:|....:||..:.||...::.
Zfish   234 CVVISRVVSGGTAEKSNLLSEGDEILEINNIPVRGKTVNDVHDLLSSMYGSLTFLLIPNSHNKLP 298

  Fly   260 SGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLPCRDIGLPFKSGDILQIINVKD 324
            ..|.||                    .:::|.|:|:||||..:||:::||.|:.||||.:.:..|
Zfish   299 LDRPTV--------------------MHVKANFSYDPSEDPYVPCKELGLSFQRGDILHVTSQDD 343

  Fly   325 PNWWQAKNITAESDK--IGLIPSQELEERRKAFVAPEAD----YVHKIGICGTRISKRKRKTMYR 383
            ||||||.....:..|  .||||.:..::.|:|......|    |..|:. |..|..|.::|.:|.
Zfish   344 PNWWQAYREGHDDQKPLAGLIPGKNFQQHREAMKKTITDKNEEYKGKLW-CAIRSKKHRKKLLYN 407

  Fly   384 SVANCEFDKAELLLYEEVT--RMPPFRRKTLVLIGVSGVGRRTLKNRLINSDVDKFGAVIPHTSR 446
            ...|.|....::|.||||:  |.||.|::.:.|||.:..|:..|:.||::|:.::|.|.:|||:|
Zfish   408 PNKNIEHYSEDILTYEEVSLYRQPPDRKRPVALIGPANSGQDELRQRLLSSEPERFAAAVPHTTR 472

  Fly   447 PKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNGNLYGTHLQSIKDVINSGRMCILDCAPNA 511
            ..|..|.||..|.|:.|:..|..:...:|:|.||:..|||||:..|::.|:|||::|:|...|.:
Zfish   473 SPRVHEVNGREYNFVSRQSFESELSAGKFIESGEYGNNLYGTNADSVRQVVNSGKICLLCLQPRS 537

  Fly   512 LKILHNSQELMPFVIFV--------AAPGMEQLKTIYADRRATGSNRNLSFDRQSSIRFSSRRAR 568
            |::|.:| :|.|::||:        ..|..|:..|:.    |.|..:.:..|    :|....:||
Zfish   538 LQVLCSS-DLKPYIIFIRPSPPPLSPPPPQERFSTML----ARGVKKRMPED----VREMMEKAR 593

  Fly   569 TLESLASLYEDDDLVATVEESSFVQRKYEKYFDMVIVNEDFDETFRQVVETLDQMSHEEQWVPVN 633
            .:|.                      .|...||..|.|.:.:::..:::..:.::.:|.||:|..
Zfish   594 EMED----------------------SYGHLFDTFITNNEPEKSVCELLSIIQKLDNEPQWIPSA 636

  Fly   634 W 634
            |
Zfish   637 W 637

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vari	NP_724288.3	L27	<105..128	CDD:280918	12/22 (55%)
		PDZ_signaling	173..250	CDD:238492	28/78 (36%)
		SH3_MPP	287..347	CDD:212796	28/61 (46%)
		Guanylate_kin	408..622	CDD:279019	62/221 (28%)
		GuKc	418..622	CDD:214504	58/211 (27%)
pals1b	NP_001171218.2	L27_N	86..133	CDD:312557	9/49 (18%)
		L27	156..197	CDD:197794	16/48 (33%)
		PDZ_signaling	211..290	CDD:238492	28/79 (35%)
		SH3_MPP5	306..367	CDD:212969	28/60 (47%)
		Guanylate_kin	434..626	CDD:331880	62/222 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0194
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810