Protein Alignment dia and for3

DIOPT Version :9

• Sequence 1: NP_001246113.1
• Gene: dia / 35340
• FlyBaseID: FBgn0011202
• Length: 1098
• Species: Drosophila melanogaster
• Sequence 2: NP_588046.1
• Gene: for3 / 2539487
• PomBaseID: SPCC895.05
• Length: 1461
• Species: Schizosaccharomyces pombe
• Alignment Length: 1401
• Identity: 285/1401 - (20%)
• Similarity: 489/1401 - (34%)
• Gaps: 417/1401 - (29%)

  Fly    23 DSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFE---------------QYIQQLSV- 71
            :|.:...|..:.||.|:.:|:.     ::|...:....|||               :|:...:.
pombe    20 NSSYSTSSNERIGTPSTISLSE-----NSDLSKLQSTNDFESREDLSLTSDDNNDPEYVMCYNTV 79

  Fly    72 ----AELDAKFLEIIEDMN--IPKDKR---EPLLAKS-KEERQKMIMWH-----LKGKNS-LERS 120
                .:::.|.|...|.:.  .|.:.|   :|.:.|. ..||.|...::     |..::: |:.:
pombe    80 YHQKTKINDKLLSETEQLRKIYPLESRVFPKPTIVKEITNERTKRYTFYDDDAPLTNQHTVLDEA 144

  Fly   121 ANSRFEKPID-YVEYLQNGEHSTHKVYQCVESLRVALTSNPISWIKEFGVAGIGTIEKLLARSKN 184
            ..:|..|.|| ::|.:.    .....|..:..|.|.|...||.|:.||  |....||.||:..:|
pombe   145 TYNRILKRIDFFIEKVV----EVPPTYHFLSLLNVQLRIQPIWWMDEF--AERNGIESLLSALRN 203

  Fly   185 ------NASYEKIEFEAIRCLKAIMN--------------NTWGLNVVLNPDQHSVVLLLAQSLD 229
                  .||...:|.:.|:.|...:|              :..|.| .|.....:|   |::||:
pombe   204 LGHYPERASKTPLESQIIQSLFHCLNSENCRRRYQSSAKCSVPGFN-ALGTIAETV---LSKSLN 264

  Fly   230 PRKPQTMCEALKLLASFCIVYERNGYEKVLRAI-----TTIAATSFKA-SERFRPIVDALFASDQ 288
            ..:..|.     ||...|.....:.::.|:||.     ..::.|.|.| ...|..::..:.....
pombe   265 SARMATF-----LLKFLCNKKGLSYFKAVIRAFEWLVEQKLSKTRFSAWMHSFNDVITGVRVCAD 324

  Fly   289 QDPK--------RDLAC------HSLIFINTLTNTPTDLNFRLHLRCEIMRMGLYDRLDEF---- 335
            ..|:        .|..|      .:|..|..|...|.||..|..||.|::..||...:|..
pombe   325 SSPQAIVHMDEFEDTDCLIDYLVATLALIRDLCAAPPDLQLRCALRHELLSAGLQKAIDSLLKWR 389

  Fly   336 --------------------------------TKIVEASNNENLQQHFKIFNEIRE--------- 359
                                            .|.|:...|...:.|....|..|:
pombe   390 NRHVRDALQLLIKEHNADARRFRSGSDVNNVDRKCVKKQMNYREESHTPHGNTTRKTSTPVSNNR 454

  Fly   360 ----------DDFEEFVQRFDNVTFNMDDATDCFDVLKNLVT--DTTSEPYFLSILQHLLYIRDD 412
                      |.|:....||   |.:.........:|:..||  |.......:.:|.|.|...:.
pombe   455 PTTPEQQAVWDVFQRIYTRF---TGSEGSKESFIKLLEYFVTEPDNGKIQKSMQLLTHTLEALEG 516

  Fly   413 FYFRPAYYQLIEECISQIVFHK------------GYCDPNFENRNFNIDTSLLLDDI-------- 457
            |....|...:....:||.:..|            .|.....||::.......:|..:
pombe   517 FKTAKADTNVGLTILSQRLLDKLGTAEEIAEYKTKYNGAMLENKHLKEQVESMLSQLNVGPRDPM 581

  Fly   458 ------VEKAKAKESKRS------------------EEYEKKIEQLESAKQEAEAKAAHLE---- 494
                  :::.||:.:.|.                  :.|.|...|:|..:...|   .||:
pombe   582 QFLKKQLDELKAELNLRDNLLASMQREFETRYRAQIQAYNKLQSQMEHVQNSNE---QHLQPGLL 643

  Fly   495 EKV------------------------------KLMEANGV-----------------------A 506
            .||                              .::..:|:                       :
pombe   644 NKVSKSFDSVHRRNLSQDSLDAMTEQFSYHVEPNILSGSGIPVRVHTPSKTEDLDESFSGSEISS 708

  Fly   507 APSPNKLPKVN----------IPMPPPPPGGGGAPPPPPPPMPGRAGGGPPPPPPPPMPGRAGGP 561
            :||| .||.|:          :..|||||.....|.|.|.|:       |.|||.|.|    |||
pombe   709 SPSP-LLPDVSDTVEEQQKLLLKSPPPPPPAVIVPTPAPAPI-------PVPPPAPIM----GGP 761

  Fly   562 PPPPPPPGMGG---PPPPPMPGMMRPGGG---PPPPPMMMGPMVP----------VLPHGLKPKK 610
            ||||||||:.|   |||||.|..:..||.   .|.|.....|.:.          .|....|.:|
pombe   762 PPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEPKIDETSLTEEQKIQLEEARKQRK 826

  Fly   611 KWD---------------------VKNPMKRANWKAIVPAKMSDKAFWVKCQEDKLAQD------ 648
            ..|                     ...|:||.:|:.:.|.. ....|...|....:...
pombe   827 AADDAARAAIEKYTSIPSLRDLHKPTRPLKRVHWQRVDPLP-GPNVFTKFCLNFDITAKVFIDNG 890

  Fly   649 --DFLAELAVKFSSKPVKKEQKDAV---DKPTTLTKKNVDLRVLDSKTAQNLAIMLGGSLKHLSY 708
              |||.|   ||.:.|  :|...||   |:.::|....|:         ||:||:| .|:.::..
pombe   891 LLDFLDE---KFDNTP--REDFVAVEISDQRSSLLPDTVE---------QNMAIIL-RSVSNMPV 940

  Fly   709 EQIKICLLRCDTDILSSNIL--------------QQLIQYLPPPEHLKRLQEIKAKGEPLPPIEQ 759
            |.: :.....:.|.|.::||              ...|.|  ..::.|     |...||...:..
pombe   941 EDL-VQKFLVEPDFLPASILYFDRASLASTNAYTDPFIPY--STDYTK-----KNPKEPTADVNS 997

  Fly   760 -------FAATIGEIKR-LSPRLHNLNFKLT-YADM-VQDIKPDIVAGTAACEEIRNSKKFSKIL 814
                   |...:..::. ...|:..|.|:.| :.|: :.:::...|..|:  :.|...|.|::..
pombe   998 LSYFEKFFVLFVVNLRHYFQERMKALKFRSTLFGDLEILEVRMKEVIDTS--DSIMEDKNFAEFF 1060

  Fly   815 ELILLLGNYMNSGSKNEAAFGFEISYLTKLSNTKDADNKQTLLHYLADLVEKKFPDALNFYDDLS 879
            :::|::|||.|......:||...:.|  :|...:|:.:..||:||..:::..:||:.|.......
pombe  1061 QVLLIIGNYFNEPYDRASAFSLYMIY--RLETLRDSSSALTLMHYFDEIIRTRFPELLQAESTFK 1123

  Fly   880 HVNKASRVNMDAIQKAMRQMNSAVKNLETDLQNNKVPQCD----DDKFSEVMGKFAEECRQQVDV 940
            .:...|..|:||:...:........:.:|.|::..:.:||    |||..:::.::..|.::::..
pombe  1124 KIQSVSGYNIDAMVAGVDGAYDEFCDFQTSLKDGALSKCDQHHPDDKAYDILSEWLPEAKERIRN 1188

  Fly   941 LGKMQLQM----EKLYKDLSEYYAFDPSKYTMEEFFADIKTFKDAFQAAHNDNVRVREELEKKRR 1001
            :.|::..|    |...|.|.||.:.|..:   ..||.::.:|.:.:..|..:| ..|.|.||:|.
pombe  1189 IKKLKTDMLTKLENTVKYLCEYDSIDKVR---NSFFKNLNSFYEMYSIAKAEN-EERFEKEKRRI 1249

  Fly  1002 LQEAREQSAREQQE----RQQRKKAVVDMDAPQTQE------GVMDSLLEALQTGSAF------G 1050
            :.|.|::..|.:|:    .:.|.|..:..|:...|:      ..::::.|.::..|..      |
pombe  1250 MSEDRDKLIRGRQKTSIVAKYRNKRELPEDSDDKQDTASKDKNSLETIDEKMEDASKIEGDAKTG 1314

  Fly  1051 QRN------RQARRQRPAGAERR----AQLSRSRSR 1076
            ..|      :....::|..||.:    ..:|..|||
pombe  1315 DDNEMEDLDKMEDLEKPDYAEEKDPYITVMSELRSR 1350

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
dia	NP_001246113.1	Drf_GBD	66..248	CDD:283920	48/219 (22%)
		Drf_FH3	253..453	CDD:283917	49/288 (17%)
		FH2	608..983	CDD:280362	90/438 (21%)
for3	NP_588046.1	Drf_FH3	307..511	CDD:283917	35/206 (17%)
		FH2	846..1295	CDD:214697	103/480 (21%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	79	1.000	Domainoid score	I2351
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	158	1.000	Inparanoid score	I1262
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000085
OrthoInspector	1	1.000	-	-		otm47407
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3069
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	5.080